LOCUS       sequence-443933-        4686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the Rotavirus RRV NSP1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4686)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP1RRV
FEATURES             Location/Qualifiers
     source          1..4686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1974..1992
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2030..2052)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2152..2171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2381..2400
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2690..2708
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2690..2707
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(2776..2823)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     2819..2837
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(4594..4612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     2041 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     2101 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2161 agcagattgt actgagagtg caccattcga cgctctccct tatgcgactc ctgcattaag
     2221 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcaagg
     2281 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     2341 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     2401 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
     2461 tctggctagc gatgaccctg ctgattggtt cgctgaccat ttccgggtgc gggacggcgt
     2521 taccagaaac tcagaaggtt cgtccaacca aaccgactct gacggcagtt tacgagagag
     2581 atgatagggt ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt
     2641 tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac
     2701 actatagaat acaagcttgc atgcctgcag gtcgactcta gaggatctcg atccggatat
     2761 agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     2821 tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc
     2881 cggatccttt tttttgagct ctcccttagc catccgagtg gacgacgtcc tccttcggat
     2941 gcccaggtcg gaccgcgagg aggtggagat gccatgccga cccggttcac agtattttgc
     3001 cagctaggcg ctactctagt ctggacggtc ttgtgatggc taaatactgt ataattagat
     3061 agtgacataa ttgcggccgc ttagtcgtca tcttcagagt ccgagatcag aagctcatat
     3121 tcttcagata gttttccttc ttcttccaat ttaaccaatg catcagtctg ttcagtgcat
     3181 aacggcaagt gatctaattc agcaacatca caccattggt tcacaatccc tttaattata
     3241 gaattgaact gattcaatgt gaaagtaaat ggaagagcct cggtttccag tatcatttta
     3301 atgtgcatat ataatgcgat atctatttgc caacagaact ttattacctg ctgttcattt
     3361 acttgcactg gattcattgt attaaacaat ttctcaacag tttgatcaat atattcagta
     3421 attgaatgcc aaagtatatc tttaactaat ggataagctt tttcaattga actacaatgt
     3481 cctacattaa cattactttt atagcaagat tggataaagc ttaaaagttc attgtacatt
     3541 gattccatct tccaatccat gtctctgaat gcatatttta cattacacca tttacattta
     3601 gaaatttttg atgctggttg ccaatgattt gatgagatgt aatttggttt gcagtgatgt
     3661 ccaagttcaa aaatgtcagc ttttattctc cccaaattta aatcatgtgt ttcaaattgt
     3721 tttaatgatg atgttgataa tgcggcaagt attttatctt tatcattaat cagatcatga
     3781 tgtaaatcag tttgagaatc ccaggtgaat tcagtaagat tcctaagaac tatgcgactt
     3841 ttagtcttgt acttattaat caaattctgt ttggagaaat ctgagtaata gattcgtttt
     3901 agcttcattg aaggaagtct acttaatttg aagtatttgt ttgcatagat ggattgtaaa
     3961 ttatctggaa gagaactcat cctttcaaag tttacttgat ctaacaatag tttatcatat
     4021 aaatcaatgc aatttacaag tctatatggc aaatctatgc atggtgatga tccttcataa
     4081 aatccaaaaa cataaacatc ccttgagtga aacttgaaaa ctgcagcatt taaagtaatt
     4141 ggaagcagta gctgattata ccatgatttg tcaaactcat ttctacattt cctctgtttt
     4201 attgtttttt taaatttatt gataacccca tgattaattg gaaatagtaa ttcatataaa
     4261 ttaataatgc attgtaattt ttccttcgtt attggagatt caaaagttct taattttaaa
     4321 agatgtggtt cattgtccaa gaagcaacgt ttgttttgga tgcaccactg acaaacatga
     4381 gctagcgaac atccgtgaca aaaagtcaat ccagttaatt gacaacactc aatgcaccac
     4441 cctttaattt taattttatt cgaagagact ggagtccata ctgaatttgc tccaattctc
     4501 agcaattctc tgtttagttt tgtaaccctt ctataatgaa agcaagcatc cttaaaggtt
     4561 gccatggcta acacaagact tttcaaaaaa aagcctatag tgagtcgtat taacccggga
     4621 tcgatatccc cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4681 ggcgct
//
