LOCUS       sequence-443938-        3746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the Rotavirus NSP5 protein with a potentially lower 
            propensity to form viroplasms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3746)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP5SClow
FEATURES             Location/Qualifiers
     source          1..3746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..88
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(134..145)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(906..924)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      920..967
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1035..1053)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1036..1053)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1343..1362)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1572..1591)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1691..1713
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1751..1769)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1837..1941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1942..2010,2011..2802)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2160..2179)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2973..3561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3462..3481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3715..3732
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccaatacgc aaaccgcctc tccccgcgca ttggccgatt cattaatgca gggggatatc
       61 gatcccggtt aatacgactc actataggct tttaaagcgc tacagtgatg tctctcagta
      121 ttgacgtgac gagtcttcct tctatttctt caagtgtata taagaatgaa tcgttttcaa
      181 caacgtcaac tatttctgga aaatctattg gtaggagtga acagtacatt tcaccagatg
      241 cagaagcatt caataaatac atgctgtcga agtctccaga ggatattgga ccatttgatt
      301 ctgcttcaaa cgatccactc accagttttt cgattagatc gaatgcagtt aagacaaacg
      361 cagacgctgg cgtgtctatg gattcatcag cacaatcacg actttcaagt gatattggat
      421 gcgatcaaat ggatttctcc ttaaataaag gcataaaaat agatgctact ttggactcat
      481 caatatcaat atctacgact agtaaaaagg agaaatcaaa acaagagtat aaaaataaat
      541 ataagaagtg ctacccaaaa attgaagcag agtctgattc agatgaatat gtactggatg
      601 attcagatag tgatgatggt aaatgtaaga actgtaaata taagaagaaa tacttcgcat
      661 taagattgag aatgaaacaa gtcgcaatgc aattgattaa agatttgtaa gtctgacctg
      721 ggaacacact agggagctcc ccactcccgt tttgtgaccg ggtcggcatg gcatctccac
      781 ctcctcgcgg tccgacctgg gcatccgaag gaggacgtcg tccactcgga tggctaaggg
      841 agagctcaaa aaaaaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      901 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      961 ttttttgctg aaaggaggaa ctatatccgg atcgagatcc tctagagtcg acctgcaggc
     1021 atgcaagctt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     1081 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     1141 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     1201 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     1261 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     1321 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     1381 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     1441 gggggactgt tgggcgccat ctccttgcac cattccttgc ggcggcggtg ctcaacggcc
     1501 tcaacctact actgggctgc ttcttaatgc aggagtcgca taagggagag cgtcgaatgg
     1561 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     1621 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     1681 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     1741 agacggaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1801 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1861 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1921 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1981 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2041 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2101 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2161 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2221 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2281 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2341 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2401 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2461 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2521 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2581 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2641 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2701 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2761 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2821 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2881 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2941 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3001 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3061 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3121 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3181 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3241 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3301 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3361 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3421 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3481 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3541 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3601 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3661 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3721 tcagtgagcg aggaagcgga agagcg
//
