LOCUS       sequence-443939-        3896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that 
            lacks all methionines fused with the full-length SA11 NSP5 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3896)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-35aaNSP1noMSA11-NSP5(wt)
FEATURES             Location/Qualifiers
     source          1..3896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      355..943
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     844..863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1097..1114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1199..1218
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1199..1217
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1378..1389)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2184..2202)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2198..2245
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2313..2331)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2314..2331)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2621..2640)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2850..2869)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2969..2991
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3029..3047)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3115..3219
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3220..3288,3289..3896,1..184)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3438..3457)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
       61 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
      121 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
      181 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
      241 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
      301 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      361 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      421 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      481 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
      541 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      601 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      661 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      721 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      781 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      841 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      901 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      961 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1021 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     1081 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     1141 accgcctctc cccgcgcgtt ggccgattca ttaatgcagg gggatatcga tcccgggtta
     1201 atacgactca ctatagggct tttttttgaa aagtcttgtg ttagccaagg ctacttttaa
     1261 agaagcaagc tttcattatc gtagattaac tgctttaaat cggagattaa gcaacattgg
     1321 tgcaaattct atttggaagc cagttcctgc catgtctctc agtattgacg tgacgagtct
     1381 tccttctatt ccttcaacta tatataagaa tgaatcgtct tcaacaacgt caactctttc
     1441 tggaaaatct attggtagga gtgaacagta catttcacca gatgcagaag cattcaataa
     1501 atacatgctg tcgaagtctc cagaggatat tggaccatct gattctgctt caaacgatcc
     1561 actcaccagt ttttcgatta gatcgaatgc agttaagaca aacgcagacg ctggcgtgtc
     1621 tatggattca tcagcacaat cacgaccttc aagtaatgtc ggatgcgatc aagtggattt
     1681 ctccttaaat aaaggcttaa aagtaaaagc taatttggac tcatcaatat caatatctac
     1741 ggatactaaa aaggagaaat caaaacaaaa ccataaaagt aggaagcact acccaagaat
     1801 tgaagcagag tctgattcag atgattatgt actggatgat tcagatagtg atgatggtaa
     1861 atgtaagaac tgtaaatata agaagaaata cttcgcatta agaatgagaa tgaaacaagt
     1921 cgcaatgcaa ttgattgaag atttgtaaaa ttatgtcact atctaattat acagtattta
     1981 gccatcacaa gaccgtccag actagagtag cgcctagctg gcaaaatact gtgaaccggg
     2041 tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga ggacgtcgtc
     2101 cactcggatg gctaagggag agctcaaaaa aaaggatccg gctgctaaca aagcccgaaa
     2161 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     2221 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat cgagatcctc
     2281 tagagtcgac ctgcaggcat gcaagcttgt attctatagt gtcacctaaa tcgtatgtgt
     2341 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     2401 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     2461 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     2521 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     2581 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     2641 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     2701 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcacca ttccttgcgg
     2761 cggcggtgct caacggcctc aacctactac tgggctgctt cttaatgcag gagtcgcata
     2821 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2881 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     2941 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3001 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3061 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3121 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3181 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3241 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3301 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3361 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3421 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3481 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3541 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3601 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3661 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3721 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3781 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3841 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctgg
//
