LOCUS       sequence-444009-        3736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recoded gene segment 11 mutant B strain SA11 used for genome 
            packaging studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3736)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP5BSA11
FEATURES             Location/Qualifiers
     source          1..3736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(212..230)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      226..273
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(341..359)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(342..359)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(649..668)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(878..897)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     997..1019
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1057..1075)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1143..1247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1248..1316,1317..2108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1466..1485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2279..2867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2768..2787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3021..3038
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3122..3140
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3186..3197)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gattgaagat ttgtaagtct gacctgggaa cacactaggg agctccccac tcccgttttg
       61 tgaccgggtc ggcatggcat ctccacctcc tcgcggtccg acctgggcat ccgaaggagg
      121 acgtcgtcca ctcggatggc taagggagag ctcaaaaaaa aggatccggc tgctaacaaa
      181 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
      241 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatcg
      301 agatcctcta gagtcgacct gcaggcatgc aagcttgtat tctatagtgt cacctaaatc
      361 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
      421 acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa
      481 ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc
      541 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg
      601 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      661 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      721 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcaccatt
      781 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttct taatgcagga
      841 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
      901 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      961 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     1021 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     1081 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     1141 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1201 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1261 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1321 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1381 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1441 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     1501 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1561 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1621 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1681 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1741 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1801 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1861 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1921 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1981 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2041 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2101 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2161 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2401 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2641 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2701 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2761 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2821 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2881 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2941 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3001 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3061 gcaaaccgcc tctccccgcg cattggccga ttcattaatg cagggggata tcgatcccgg
     3121 ttaatacgac tcactatagg cttttaaagc gctacagtga tgtctctcag tattgacgtg
     3181 acgagtcttc cttctattcc ttcaactata tataagaatg aatcgtcttt acaaacgtca
     3241 accttatctg gaaaattaat tgatacgagt aaacagaaca tttcaccaga tgcagaaaaa
     3301 ttcaataaat acatgctacg gaagactaca gaggaaataa aaccctctca tcccctttca
     3361 aacgatccac tcaccaaatt ttctattcga tcgaatgcta aaaagacaaa tacagacgct
     3421 gaaccgtcaa aaaagtcatc agaaaaataa cgaacataaa ttaatgtaaa aagcgtcaaa
     3481 gtagatttct ccttaaataa aggcttaaaa gtaaaagcta atttggactc atcaatatca
     3541 atatctacgg atactaaaaa ggagaaatca aaacaaaacc ataaaagtag gaagcactac
     3601 ccaagaattg aagcagagtc tgattcagat gattatgtac tggatgattc agatagtgat
     3661 gatggtaaat gtaagaactg taaatataag aagaaatact tcgcattaag aatgagaatg
     3721 aaacaagtcg caatgc
//
