LOCUS       sequence-444016-        3301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the short Rotavirus SA11 NSP1 protein (35 amino acids) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3301)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-35aaNSP1SA11
FEATURES             Location/Qualifiers
     source          1..3301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     414..433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     667..684
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        769..787
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1159..1177)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1173..1220
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1288..1306)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1289..1306)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1596..1615)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1825..1844)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1944..1966
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2004..2022)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2090..2194
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2195..2263,2264..3055)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2413..2432)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3226..3301,1..513)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
       61 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
      121 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
      181 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
      241 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
      301 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
      361 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      421 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
      481 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      541 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      601 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      661 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
      721 cccgcgcgtt ggccgattca ttaatgcagg gggatatcga tcccgggtta atacgactca
      781 ctataggctt ttttttgaaa agtcttgtgt tagccatggc tacttttaaa gatgcatgct
      841 ttcattatcg tagattaact gctttaaatc ggagattatg caacattggt gcaaattcta
      901 tttggatgcc agttcctgat tgaaattatg tcactatcta attatacagt atttagccat
      961 cacaagaccg tccagactag agtagcgcct agctggcaaa atactgtgaa ccgggtcggc
     1021 atggcatctc cacctcctcg cggtccgacc tgggcatccg aaggaggacg tcgtccactc
     1081 ggatggctaa gggagagctc aaaaaaaagg atccggctgc taacaaagcc cgaaaggaag
     1141 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     1201 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatcgaga tcctctagag
     1261 tcgacctgca ggcatgcaag cttgtattct atagtgtcac ctaaatcgta tgtgtatgat
     1321 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
     1381 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
     1441 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
     1501 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
     1561 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     1621 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     1681 aggccccgtg gccgggggac tgttgggcgc catctccttg caccattcct tgcggcggcg
     1741 gtgctcaacg gcctcaacct actactgggc tgcttcttaa tgcaggagtc gcataaggga
     1801 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     1861 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     1921 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     1981 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     2041 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2101 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2161 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2221 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2281 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2341 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2401 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2461 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2521 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2581 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2641 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2701 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2761 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2821 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2881 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2941 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3001 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3061 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3121 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3181 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3241 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3301 g
//
