LOCUS       sequence-444022-        3740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recoded gene segment 11 mutant X strain SA11 used for genome 
            packaging studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3740)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP5XSA11
FEATURES             Location/Qualifiers
     source          1..3740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..28
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(66..88)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     188..207
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     417..436
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        726..744
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     726..743
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(812..859)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     855..873
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(1685..1703)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1787..1804)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1958..2546)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2038..2057)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2717..3508,3509..3577))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3340..3359
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3578..3682)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
       61 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
      121 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
      181 aactatgcgg catcagagca gattgtactg agagtgcacc attcgacgct ctcccttatg
      241 cgactcctgc attaagaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
      301 aaggaatggt gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
      361 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg
      421 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
      481 cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct gaccatttcc
      541 gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc gactctgacg
      601 gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac acaattaggc
      661 ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac
      721 atacgattta ggtgacacta tagaatacaa gcttgcatgc ctgcaggtcg actctagagg
      781 atctcgatcc ggatatagtt cctcctttca gcaaaaaacc cctcaagacc cgtttagagg
      841 ccccaagggg ttatgctagt tattgctcag cggtggcagc agccaactca gcttcctttc
      901 gggctttgtt agcagccgga tccttttttt tgagctctcc cttagccatc cgagtggacg
      961 acgtcctcct tcggatgccc aggtcggacc gcgaggaggt ggagatgcca tgccgacccg
     1021 gtcacaaaac gggagtgggg agctccctag tgtgttccca ggtcagactt acaaatcttc
     1081 aatcaattgc attgcgactt gtttcattct cattcttaat gcgaagtatt tcttcttata
     1141 tttacagttc ttacatttac catcatcact atctgaatca tccagtacat aatcatctga
     1201 atcagactct gcttcaattc ttgggtagtg cttcctactt ttatggtttt gttttgattt
     1261 ctccttttta gtatccgtag atattgatat tgatgagtcc aaattagctt ttacttttaa
     1321 gcctttattt aaggcgattt tttattacat ttaatcgaaa tttttttaag gttaatattt
     1381 tgcggttttt ttaatcatgg tttatttttc tgcatttttt ttaacttaat tcaataattt
     1441 taaattacgt tattaatgta aacttttagc attgtttaat tattgggttt attaggggtt
     1501 ttttttgtgc gtttaagttt tgaggttttg gttatttttt gtattataaa gttctatttt
     1561 aacaatataa ttttaagaaa acaaatttgt tgtagtttat atttcattct tatatatatt
     1621 ttaaacattt acattaagac tcgtcacgtc aatactgaga gacatcactg tagcgcttta
     1681 aaagcctata gtgagtcgta ttaaccggga tcgatatccc cctgcattaa tgaatcggcc
     1741 aatgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1801 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1861 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1921 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1981 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2041 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2101 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2161 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2221 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2281 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2341 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2401 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2461 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2521 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2581 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2641 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2701 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2761 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2821 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2881 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2941 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3001 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3061 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3121 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3181 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3241 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3301 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3361 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3421 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3481 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3541 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3601 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3661 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3721 ccattattat catgacatta
//
