LOCUS       sequence-444025-        3895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the protein consisting of 35 amino acid of Rotavirus SA11 
            NSP1 protein and the full-length recoded NSP5 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3895)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-35aaNSP1SA11-NSP5(io)
FEATURES             Location/Qualifiers
     source          1..3895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(920..938)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      934..981
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1049..1067)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1050..1067)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1357..1376)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1586..1605)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1705..1727
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1765..1783)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1851..1955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1956..2024,2025..2816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2174..2193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2987..3575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3476..3495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3729..3746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3831..3849
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcatgctttc attatcgtag attaactgct ttaaatcgga gattatgcaa cattggtgca
       61 aattctattt ggatgccagt tcctgatatg agtttaagta tagatgttac atcacttccc
      121 tcgatcccct caacaatata taaaaatgaa agttcaagta caacaagtac actatccgga
      181 aaatctatag gaagaagtga acaatatata agcccagatg cagaagcttt taataaatat
      241 atgctaagta aatcacctga agatataggg ccaagtgata gtgcatcaaa cgatcccctc
      301 acaagttttt caatacgaag taacgcagta aaaactaatg ccgatgcagg tgtaagcatg
      361 gactcctcag cacaatcaag accttcatct aacgtaggat gtgatcaagt cgatttctca
      421 ttaaacaaag gattaaaagt taaagcaaat ttagattcat ctataagtat aagcacagat
      481 acaaaaaaag aaaaaagtaa acaaaatcat aaaagtcgaa aacactatcc aagaatagaa
      541 gccgaaagtg atagcgatga ttatgtacta gacgacagcg attcagatga tggaaaatgt
      601 aaaaattgca aatacaaaaa aaaatatttt gcactaagaa tgagaatgaa acaagttgcc
      661 atgcaattaa ttgaagattt ataaaattat gtcactatct aattatacag tatttagcca
      721 tcacaagacc gtccagacta gagtagcgcc tagctggcaa aatactgtga accgggtcgg
      781 catggcatct ccacctcctc gcggtccgac ctgggcatcc gaaggaggac gtcgtccact
      841 cggatggcta agggagagct caaaaaaaag gatccggctg ctaacaaagc ccgaaaggaa
      901 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      961 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatcgag atcctctaga
     1021 gtcgacctgc aggcatgcaa gcttgtattc tatagtgtca cctaaatcgt atgtgtatga
     1081 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
     1141 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
     1201 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
     1261 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
     1321 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     1381 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     1441 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcaccattcc ttgcggcggc
     1501 ggtgctcaac ggcctcaacc tactactggg ctgcttctta atgcaggagt cgcataaggg
     1561 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     1621 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     1681 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     1741 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1801 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1861 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1921 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1981 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2041 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2101 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2161 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2221 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2281 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2341 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2401 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2461 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2521 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2581 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2641 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2701 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2761 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2821 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2881 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3661 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3721 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3781 tccccgcgcg ttggccgatt cattaatgca gggggatatc gatcccgggt taatacgact
     3841 cactataggc ttttttttga aaagtcttgt gttagccatg gctactttta aagat
//
