LOCUS       sequence-444080-        8673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP 
            tags in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Zhao Y, Caron C, Chan YY, Lee CK, Xu X, Zhang J, Masubuchi T, Wu C, 
            Bui JD, Hui E
  TITLE     cis-B7:CD28 interactions at invaginated synaptic membranes provide 
            CD28 co-stimulation and promote CD8(+) T cell function and 
            anti-tumor immunity.
  JOURNAL   Immunity. 2023 Jun 13;56(6):1187-1203.e12. doi: 
            10.1016/j.immuni.2023.04.005. Epub 2023 May 8.
  PUBMED    37160118
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2023.04.005"; journalName: "Immunity"; date: 
            "2023-06-13- 13"; volume: "56"; issue: "6"; pages: "1187-1203.e12"
COMMENT     Sequence Label: FKBP:FRB-FBAR
FEATURES             Location/Qualifiers
     source          1..8673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      597..606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(603..605,606..608,609..1319)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(648..669)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(909..928)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1256..1277
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1359..1637
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             1668..1709
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    2736..2857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2864..3319)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3001..3022)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3213..3232
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     enhancer        3342..3645
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3646..3849
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3878..3887
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3884..3886,3887..4207)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             4250..4570
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     gap             4613..4624
                     /estimated_length=12
     CDS             4625..4945
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     misc_feature    5734..5799
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    5923..6044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6071..6175
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6177..6534
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6177..6368
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6200..6220)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6385..6520
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6569..7363
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6569..7363
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6623..6642)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7233..7252
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7551..7570)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7595..7642
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7971..8559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8460..8479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      601 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      721 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      781 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      841 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      901 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      961 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1021 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1081 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1141 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1201 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1261 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     1321 gcagtagcgg aagcagtggt tcttcaggat catccatgat cctctggcat gagatgtggc
     1381 atgaaggcct ggaagaggca tctcgtttgt actttgggga aaggaacgtg aaaggcatgt
     1441 ttgaggtgct ggagcccttg catgctatga tggaacgggg cccccagact ctgaaggaaa
     1501 catcctttaa tcaggcctat ggtcgagatt taatggaggc ccaagagtgg tgcaggaagt
     1561 acatgaaatc agggaatgtc aaggacctcc tccaagcctg ggacctctat tatcatgtgt
     1621 tccgacgaat ctcaaagggg tgtggcagcg gaggtagtgg gagcggtggc aagcccatcc
     1681 ccaaccccct gctgggcctg gacagcacca agggctcggg ctcgacctcg ggctcgggca
     1741 ccggtatgag ctggggcacc gagctctggg atcagtttga caacttagaa aaacacacac
     1801 agtggggaat tgatattctt gagaaatata tcaagtttgt gaaagaaagg acagagattg
     1861 aactcagcta tgcaaagcaa ctcaggaatc tttcaaagaa gtaccaacct aaaaagaact
     1921 cgaaggagga agaagaatac aagtatacgt catgtaaagc tttcatttcc aacctgaacg
     1981 aaatgaatga ttacgcaggg cagcatgaag ttatctccga gaacatggca tcacagatca
     2041 ttgtggactt ggcacgctat gttcaggaac tgaaacagga gaggaaatca aactttcacg
     2101 atggccgtaa agcacagcag cacatcgaga cttgctggaa gcagcttgaa tctagtaaaa
     2161 ggcgatttga acgcgattgc aaagaggcgg acagggcgca gcagtacttt gagaaaatgg
     2221 acgctgacat caatgtcaca aaagcggatg ttgaaaaggc ccgacaacaa gctcaaatac
     2281 gtcaccaaat ggcagaggac agcaaagcag attactcatc cattctccag aaattcaacc
     2341 atgagcagca tgaatattac catactcaca tccccaacat cttccagaaa atacaagaga
     2401 tggaggaaag gaggattgtg agaatgggag agtccatgaa gacatatgca gaggttgatc
     2461 ggcaggtgat cccaatcatt gggaagtgcc tggatggaat agtaaaagca gccgaatcaa
     2521 ttgatcagaa aaatgattca cagctggtaa tagaagctta taaatcaggg tttgagcctc
     2581 ctggagacat tgaatttgag gattacactg ggtagcggcc gcgactctag atcataatca
     2641 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2701 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     2761 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2821 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
     2881 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     2941 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     3001 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     3061 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     3121 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     3181 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     3241 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     3301 gcgccgctac agggcgcgtc aggtggcact tttcggggcc gcgttacata acttacggta
     3361 aatggcccgc ctgactgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     3421 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     3481 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     3541 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     3601 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     3661 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     3721 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     3781 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     3841 agcagagctg gtttagtgaa ccgtcagatc cgctagcgcc accatgggag tgcaggtgga
     3901 aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca
     3961 ctacaccggg atgcttgaag atggaaagaa atttgattcc tcccgggaca gaaacaagcc
     4021 ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca
     4081 gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac
     4141 tgggcaccca ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa
     4201 actggaactg caggatatca agcttgcggt accgcgggcc cgggatccag gagtgcaggt
     4261 ggaaaccatc tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt
     4321 gcactacacc gggatgcttg aagatggaaa gaaatttgat tcctcccggg acagaaacaa
     4381 gccctttaag tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc
     4441 ccagatgagt gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc
     4501 cactgggcac ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct
     4561 aaaactggaa ctgcaggata tcaagcttgc ggtaccgcgg gcccgggatc cannnnnnnn
     4621 nnnnggagtg caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg
     4681 ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc
     4741 ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg
     4801 ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga
     4861 ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt
     4921 cgatgtggag cttctaaaac tggaactgca ggatatcaag cttgcggtac cgcgggcccg
     4981 ggatccaatg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     5041 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     5101 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     5161 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     5221 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
     5281 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     5341 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
     5401 cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa
     5461 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata
     5521 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
     5581 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
     5641 ggccagatac tgcgacctcc ctagcaaact ggggcacggt aaaaagaaga aaaagaagtc
     5701 aaagacaaag tgtgtaatta tgtagtgtac aagtccggac tcagatctcg agctcaagct
     5761 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc
     5821 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     5881 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     5941 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6001 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
     6061 cggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6121 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     6181 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     6241 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     6301 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6361 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6421 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     6481 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     6541 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     6601 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     6661 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6721 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     6781 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6841 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6901 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6961 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     7021 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     7081 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     7141 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     7201 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     7261 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     7321 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     7381 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     7441 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7501 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     7561 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     7621 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     7681 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     7741 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     7801 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     7861 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7921 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7981 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8041 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8101 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     8161 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8221 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8281 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8341 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8401 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8461 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8521 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8581 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8641 gattctgtgg ataaccgtat taccgccatg cat
//
