LOCUS       sequence-444102-        5908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  replicative E. coli-S. pyogenes shuttle plasmid for 
            erythromycin-inducible expression of the Firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Lautenschlager N, Schmidt K, Schiffer C, Wulff TF, Hahnke K, 
            Finstermeier K, Mansour M, Elsholz AKW, Charpentier E
  TITLE     Expanding the genetic toolbox for the obligate human pathogen 
            Streptococcus pyogenes.
  JOURNAL   Front Bioeng Biotechnol. 2024 Jun 7;12:1395659. doi: 
            10.3389/fbioe.2024.1395659. eCollection 2024.
  PUBMED    38911550
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2024-06-7"; volume: "12:1395659. doi"; 
            pages: " 10.3389/fbioe.2024.1395659. eCollection 2024"
COMMENT     Sequence Label: pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
FEATURES             Location/Qualifiers
     source          1..5908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4..20)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4..20)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2944..2955
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(3710..3744)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     3780..3796
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4245..5894
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4308..4326)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tgtgtcatag ctgtttcctg caaagaatgg tgatgacaat tgtacaggtt atcaataaag
       61 ccattgatac tctcattctt atcccgtatt tattaacttc tcctagtatt agtaattatc
      121 atggctgtca tggcgcatta acggaataaa gggtgtgctt aaatcgggcc attttgcgta
      181 ataagaaaaa ggattaatta tgagcgaatt gaattaataa taaggtaata gatttacatt
      241 agaaaatgaa aggggatttt atgcgtgaga atgttacagt ctatcccggc aatagttacc
      301 cttattatca agataagaaa gaaaaggatt tttcgctacg ctcaaatcct ttaaaaaaac
      361 acaaaagacc acatttttta atgtggtctt ttattcttca actaaagcac ccattagttc
      421 aacaaacgaa aattggataa agtgggatat ttttaaaata tatatttatg ttacagtaat
      481 attgactttt aaaaaaggat tgattataat gaagaaagca gacaagtaag cctcctaaat
      541 tcactttaga taaaaattta ggaggcatat caaatgaact ttaataaaat tcactttaga
      601 taaaaattta ggaggcatat caaatgaact ttaataaaat tgatttagac aattggaaga
      661 gaaaagagat atttaatcat tatttgaacc aacaaacgac ttttagtata accacagaaa
      721 ttgatattag tgttttatac cgaaacataa aacaagaagg atataaattt taccctgcat
      781 ttattttctt agtgacaagg gtgataaact caaatacagc ttttagaact ggttacaata
      841 gcgacggaga gttaggttat tgggataagt tagagccact ttatacaatt tttgatggtg
      901 tatctaaaac attctctggt atttggactc ctgtaaagaa tgacttcaaa gagttttatg
      961 atttatacct ttctgatgta gagaaatata atggttcggg gaaattgttt cccaaaacac
     1021 ctatacctga aaatgctttt tctctttcta ttattccatg gacttcattt actgggttta
     1081 acttaaatat caataataat agtaattacc ttctacccat tattacagca ggaaaattca
     1141 ttaataaagg taattcaata tatttaccgc tatctttaca ggtacatcat tctgtttgtg
     1201 atggttatca tgcaggattg tttatgaact ctattcagga attgtcagat aggcctaatg
     1261 actggctttt ataatatgag ataatgccga ctgtactttt tacagtcggt tttctaatgt
     1321 cactaacctg ccccgttagt tgaagaaggt ttttatatta cagctccaga tccatatcct
     1381 tctttttctg aaccgacttc tcctttttcg cttctttatt ccaattgctt tattgacgtt
     1441 gagcctcgga acccttaaca atcccaaaac ttgtcgactt tcgtcagggg ggcttttatt
     1501 tattcaataa tccctcctct caataaatct attgttgtac ttaattcaac ttccatttct
     1561 ctgtatcttt caatacgctc ttttaagtcc ttaatttctt tttttaattc ctcattttca
     1621 gcaaataact ctttttcttt gtttgtcatt ttatttcccc cgtttcagca tcaagaacct
     1681 ttgcataact tgctctatat ccacactgat aattgccctc aaaccataat ctaaaggcgc
     1741 tagagtttgt tgaaacaata tcttttacat cattcgtatt taaaattcca aactccgctc
     1801 ccctaaggcg aataaaagcc attaactctt ttgtatttac caaattatag tcatccacta
     1861 tatctaaaag taaattcttc aattttcttt tttggctttc atcaagtgtt atatagcggt
     1921 caatatcaaa atcattaatg ttcaaaatat cttttttgtc gtatatatgt ttattcttag
     1981 caatagcgtc ctttgattca tgagtcaaat attcatatga acctttgata taatcaagta
     2041 tctcaacatg agcaactgaa ctattcccca attttcgctt aatcttgttc ctaacgcttt
     2101 ctattgttac aggatttcgt gcaatatata taacgtgata gtgtggtttt ttatagtgct
     2161 ttccatttcg tataacatca ctactattcc atgtatcttt atcttttttt tcgtccatat
     2221 cgtgtaaagg actgacagcc atagatacgc ccaaactctc taatttttct ttccaatcat
     2281 taggaattga gtcaggatat aataaaaatc caaaatttct agctttagta tttttaatag
     2341 ccatgatata attaccttat caaaaacaag tagcgaaaac tcgtatcctt ctaaaaacgc
     2401 gagctttcgc ttattttttt tgttctgatt cctttcttgc atattcttct atagctaacg
     2461 ccgcaaccgc agattttgaa aaaccttttt gtttcgccat atctgttaat tttttatctt
     2521 gctcttttgt cagagaaatc ataactcttt ttttcgattc tgaaatcacc atttaaaaaa
     2581 ctccaatcaa ataattttat aaaattagtg tatcactttg taatcataaa aacaacaata
     2641 aagctactta aatatagatt tataaaaaac gttggcgatt cgttggcgat tgaaaaaccc
     2701 ctcaaaccct tgagccagtt gggatagagc gtttttggca caaaaattgg cactcggcac
     2761 ttaatggggg gtcgtagtac ggaagcaaaa ttcgcttcct ttccccccat ttttttccaa
     2821 attccaaatt tttttcaaaa attttccagc gctaccgctc ggcaaaattg caagcaattt
     2881 ttaaaatcaa acccatgagg gaatttcatt ccctcaaact cccttgagcc tcctccaacc
     2941 gaaatagaag gacgctgcgc ttattatttc attcagtcat cggctttcat aatctaacag
     3001 acaacatctt cgctgcaaag ccacgctacg ctcaagggct tttacgctac gataacgcct
     3061 gttttaacga ttatgccgat aactaaacga aataaacgct aaaacgtcac agaaacgatt
     3121 ttcagacgtt ttaataaaaa atcgaccgaa aaataaatat aaatcggata tacaatcggc
     3181 aattgacgaa actgcaaaat atcctgtaaa ggatacggat tttatgaccg atgatgaaga
     3241 aaagaatttg aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa ggttaatctc
     3301 ctatggtggt ttgttaaaag aaatacataa aaaattaaac cttgatgaca cagaagaagg
     3361 cgatttgatt catacagatg atgacgaaaa agccgatgaa gatggatttt ctattattgc
     3421 aatgtggaat tgggaacgga aaaattattt tattaaagag tagttcaaca aacgggccag
     3481 tttgttgaag attagatgct ataattgtta ttaaaaggat tgaaggatgc ttaggaagac
     3541 gagttattaa tagctgaata agaacggtgc tctccaaata ttcttattta gaaaagcaaa
     3601 tctaaaatta tctgaaaagg gaatgagaat agtgaatgga ccaataataa tgactagaga
     3661 agaaagaatg aagattgttc atgaaattaa ggaacgaata ttggataaca taaaaaacgc
     3721 ccggcggcaa ccgagcgttc tgaattatgg ggatgatgtt aaggctattg gtgtttatgg
     3781 taaaacgacg gccagtcgcc tatcattttc aatgaaagaa gtcactaata aaatgtgaaa
     3841 aaatttgcaa acaaattgaa atatcataga aaactattga aaacgctgag tgtcttgata
     3901 tttttgatac cgatttatca tcgcaatttt acctaaatta tggtacaatg taagaggaag
     3961 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
     4021 aatgcgtaat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
     4081 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
     4141 gataactaaa attacaaaca aatcgtttaa cttctgtatt tatttataga tgtaatcact
     4201 tcaggagtga ttacatgaac aaaaatataa aatattctca aaacgaagac gccaaaaaca
     4261 taaagaaagg cccggcgcca ttctatcctc tagaggatgg aaccgctgga gagcaactgc
     4321 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
     4381 tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg gcagaagcta
     4441 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
     4501 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     4561 acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt
     4621 ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc
     4681 agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     4741 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca gagtcctttg
     4801 atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg ttacctaagg
     4861 gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga gatcctattt
     4921 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     4981 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     5041 gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa agtgcgttgc
     5101 tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa tacgatttat
     5161 ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc ggggaagcgg
     5221 ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact gagactacat
     5281 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     5341 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaga
     5401 gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac aatccggaag
     5461 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     5521 acgaagacga acacttcttc atagttgacc gcttgaagtc tttaattaaa tacaaaggat
     5581 atcaggtggc ccccgctgaa ttggaatcga tattgttaca acaccccaac atcttcgacg
     5641 cgggcgtggc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     5701 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     5761 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     5821 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     5881 agtccaaatt gtaatactag tactgcag
//
