LOCUS       sequence-444103-        8318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of MARK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Mendoza CS, Plowinske CR, Montgomery AC, Quinones GB, Banker G, 
            Bentley M
  TITLE     Kinesin Regulation in the Proximal Axon is Essential for 
            Dendrite-selective Transport.
  JOURNAL   Mol Biol Cell. 2024 Jun 1;35(6):ar81. doi: 10.1091/mbc.E23-11-0457. 
            Epub 2024 Apr 10.
  PUBMED    38598291
REFERENCE   2  (bases 1 to 8318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-06-1"; volume: "35(6):ar81. doi"; pages: " 
            10.1091/mbc.E23-11-0457. Epub 2024 Apr 10"
COMMENT     Sequence Label: pBa.Mark2-3myc
FEATURES             Location/Qualifiers
     source          1..8318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(107..695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(187..206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(866..1657,1658..1726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1489..1508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1727..1831)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1899..1917
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1955..1977)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2077..2096
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2290..2312
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2304..2321
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2305..2321
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5338..5360
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    5425..5449
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7858..7992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7895..7914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7949..7968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8023..8039)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8023..8039)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8036..8058)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8047..8063
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8071..8077,8078..8095,8096..8101))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8116..8137
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8254..8271)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
       61 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      121 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      181 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      241 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      301 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      361 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      421 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      481 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      541 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      601 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      661 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      721 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      781 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
      841 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
      901 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
      961 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1021 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1081 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1141 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     1201 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1261 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1321 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1381 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     1441 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     1501 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     1561 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     1621 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     1681 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     1741 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     1801 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     1861 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     1921 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     1981 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     2041 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     2101 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     2161 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     2221 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     2281 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagttg ggatctttgc
     2341 attggcccac ggctctcagg atggggatgc tccccttcag cacccggttc cccttggaaa
     2401 ctgatggtcc tggctctgtg gcatggcagt ggcactgtga ggagccccct accagcagca
     2461 cacagtgggg ttggcactgc cacgctccgg atgccgcgct ctgatccaac cccataatca
     2521 agggaacccg aattgcccca tcattgcccc caccaccccc atcctgccgg gccctcacac
     2581 cccacgctgc cttctggtga cattccccag cccaaaccca cggcttcatg gctaccgcgg
     2641 ggcatttccc attgccgccc cattatcagc tctgcacacc tcccgctgta cccatgcctc
     2701 gtggctgccc ttctttgacg tataatcttc taattaatac ccggccttgt caaagtggag
     2761 cacaaacgtt aattaattcc ccagcaggca ggtaattaac agtgtgactc cctttttgct
     2821 gcgagtgggg ctgatacaga gagatgtggc actatggagc ccacggggtc ctggcactgg
     2881 gtgcccacgg aggtccccat gtgctgcagt gtcaccgcct ccgaggagac agtattgtcc
     2941 ctgcggtgtc cctgcagctc agctctgtcc acagggccac ctccagtttg gaggggacac
     3001 aatgcagccc cgatgcaacc catcctcgca gcatcccagg gacaaagacc ccactgcaag
     3061 accgcacaca gggctgggtc ccgctcccct aatatctaca gtgcttcttg catggcccct
     3121 taatcaatgc agttaatcag catgcgctca tgcaccgctc tggagctgca aagcccctcg
     3181 cagcgctgct caccaacacc gcgcaccgcc ccggcccagc ctgcagcacg cgctgcaaac
     3241 aggaaagaaa caaaatattg cccaaatgta ggcaaaggca ttcggctgcc ttgacctccg
     3301 ccgggccggg ccctgcctga ctcagctcct tactcagcgc tcgcttcctc cctccggctg
     3361 ccaccgccgc agcgcacacc ctgacaaaga gtggccctta acgggctctg aggtgcaccc
     3421 agcagtgcac tcagcagtcc aagggccggc ctggaggttt gcaccgctac gtgctgacat
     3481 tagcattgaa cttggccctg ggtagtgctg caggccgggc ggggtgggtg tagagagtgc
     3541 agcgcgcgtt gcacccggtg ccccttcccc tcccttgcat cccagcaggc tgcaccccag
     3601 caccaggccc gtgcatgcat gctcctggtg ttattgcagc ctggtgcatg catgcgtctt
     3661 gatggtgcag cgctgtgcat gcatcctcct tggtgtgtag cagtctagtg catgcatacc
     3721 cctcggtgtt attgctgctc tgtgcacgca cgctcattgt atcacttcat cccagtgcat
     3781 gcactcacac tggagcgatt gctgctcggt gcacgcacac tcattgtatc actgcagctc
     3841 agtggctgca cgcacaccgg tgttattgct gctcggtgcg tgcatgcaca tcagtgtcgc
     3901 tgcagctcag tgcatgcacg ctcattgccc atcgctatcc ctgcctctcc tgctggcgct
     3961 ccccgggagg tgacttcaag gggaccgcag gaccacctcg ggggtggggg gagggctgca
     4021 cacgcggacc ccgctccccc tccccaacaa agcactgtgg aatcaaaaag gggggagggg
     4081 ggatggaggg gcgcgtcaca cccccgcccc acaccctcac ctcgaggtga gccccacgtt
     4141 ctgcttcact ctccccatcc cccccccctc cccacccccc atttttgatt tatttatttt
     4201 ttaattattt tgtggcgcgg tggggggggg gggggggggg ggggcggggg gggggggggg
     4261 gggctggcgc caccaggagc ggcgcgcccg caaagtgtcc ttgtgtggcg gggcggcggc
     4321 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc
     4381 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     4441 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     4501 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
     4561 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     4621 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     4681 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     4741 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     4801 ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc gaggtgctgg
     4861 ggacgggccg gcgtcggggg gggggggccg gggggggcgg gggggggggg gcgggggggg
     4921 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg ggggcggggg
     4981 gcgggcgggg gggcgcggcc cgcccgcgcg cggggggggg cgggggggag gggcgcggcg
     5041 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     5101 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     5161 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     5221 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc
     5281 agcctcgggg ctgtccgcag ggggacggct gccttcgggg gggacggggc agggcggggt
     5341 tcggcttctg gcgtgtgacc ggcggggttt atatcttccc ttctctgttc ctccgcagcc
     5401 cccaagcttg gcgcgcctat aatgccaact ttgtacaaaa aagttggcac catgtccagc
     5461 gctcggaccc ccctacccac gctgaacgag agggacacgg agcagcccac cttgggacac
     5521 cttgactcca agcccagcag taagtccaac atgattcggg gccgcaactc agccacctct
     5581 gctgatgagc agccccacat tggaaactac cggctcctca agaccattgg caagggtaat
     5641 tttgccaagg tgaagttggc ccgacacatc ctgactggga aagaggtagc tgtgaagatc
     5701 attgacaaga ctcaactgaa ctcctccagc ctccagaaac tattccgcga agtaagaata
     5761 atgaaggttt tgaatcatcc caacatagtt aaattatttg aagtgattga gactgagaaa
     5821 acgctctacc ttgtcatgga gtacgctagt ggcggagagg tatttgatta cctagtggct
     5881 catggcagga tgaaagaaaa agaggctcga gccaaattcc gccagatagt gtctgctgtg
     5941 cagtactgtc accagaagtt tattgtccat agagacttaa aggcagaaaa cctgctcttg
     6001 gatgctgata tgaacatcaa gattgcagac tttggcttca gcaatgaatt cacctttggg
     6061 aacaagctgg acaccttctg tggcagtccc ccttatgctg ccccagaact cttccagggc
     6121 aaaaaatatg atggacccga ggtggatgtg tggagcctag gagttatcct ctatacactg
     6181 gtcagcggat ccctgccttt tgatggacag aacctcaagg agctgcggga acgggtactg
     6241 aggggaaaat accgtattcc attctacatg tccacggact gtgaaaacct gcttaagaaa
     6301 tttctcattc ttaatcccag caagagaggc actttagagc aaatcatgaa agatcgatgg
     6361 atgaatgtgg gtcacgaaga tgatgaacta aagccttacg tggagccact ccctgactac
     6421 aaggaccccc ggcggacaga gctgatggtg tccatgggtt atacacggga agagatccag
     6481 gactcgctgg tgggccagag atacaacgag gtgatggcca cctatctgct cctgggctac
     6541 aagagctccg agctggaagg cgacaccatc accctgaaac cccggccttc agctgatctg
     6601 accaatagca gcgccccatc cccatcccac aaggtacagc gcagcgtgtc ggccaatccc
     6661 aagcagcggc gcttcagcga ccaggctggt cctgccattc ccacctctaa ttcttactct
     6721 aagaagactc agagtaacaa cgcagaaaat aagcggcctg aggaggaccg ggagtcaggg
     6781 cggaaagcca gcagcacagc caaggtgcct gccagccccc tgcccggtct ggagaggaag
     6841 aagaccaccc caaccccctc cacgaacagc gtcctctcca ccagcacaaa tcgaagcagg
     6901 aattccccac ttttggagcg ggccagcctc ggccaggcct ccatccagaa tggcaaagac
     6961 agcacagccc cccagcgtgt ccctgttgcc tccccatccg cccacaacat cagcagcagt
     7021 ggtggagccc cagaccgaac taacttcccc cggggtgtgt ccagccgaag caccttccat
     7081 gctgggcagc tccgacaggt gcgggaccag cagaatttgc cctacggtgt gaccccagcc
     7141 tctccctctg gccacagcca gggccggcgg ggggcctctg ggagcatctt cagcaagttc
     7201 acctccaagt ttgtacgcag gaacctgaat gaacctgaaa gcaaagaccg agtggagacg
     7261 ctcagacctc acgtggtggg cagtggcggc aacgacaaag aaaaggaaga atttcgggag
     7321 gccaagcccc gctccctccg cttcacgtgg agtatgaaga ccacgagctc catggagccc
     7381 aacgagatga tgcgggagat ccgcaaggtg ctggacgcga acagctgcca gagcgagctg
     7441 catgagaagt acatgctgct gtgcatgcac ggcacgccgg gccacgagga cttcgtgcag
     7501 tgggagatgg aggtgtgcaa actgccgcgg ctctctctca acggggttcg atttaagcgg
     7561 atatcgggca cctccatggc cttcaaaaac attgcctcca aaatagccaa cgagctgaag
     7621 cttttgccaa ctttcttgta catgcagaag ctgatctcag aggaggacct gcaaaaactt
     7681 atttctgaag aggatctgca aaagcttatt tctgaagaag atctgcggac cgtctaatct
     7741 agataagtaa tgatcataat cagccatatc acatctgtag aggttttact tgctttaaaa
     7801 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     7861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7981 catgtctgga tccccgggta ccgagctcga attcgtaatc atgtcatagc tgtttcctgt
     8041 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8101 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8161 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8221 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8281 cgttcggctg cggcgagcgg tatcagctca ctcaaagg
//
