LOCUS       sequence-444105-        5826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  replicative E. coli-S. pyogenes shuttle plasmid, constitutive 
            expression of firefly luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Lautenschlager N, Schmidt K, Schiffer C, Wulff TF, Hahnke K, 
            Finstermeier K, Mansour M, Elsholz AKW, Charpentier E
  TITLE     Expanding the genetic toolbox for the obligate human pathogen 
            Streptococcus pyogenes.
  JOURNAL   Front Bioeng Biotechnol. 2024 Jun 7;12:1395659. doi: 
            10.3389/fbioe.2024.1395659. eCollection 2024.
  PUBMED    38911550
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2024-06-7"; volume: "12:1395659. doi"; 
            pages: " 10.3389/fbioe.2024.1395659. eCollection 2024"
COMMENT     Sequence Label: pEC2861 (pSpy1C-PgyrA_ffluc)
FEATURES             Location/Qualifiers
     source          1..5826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4..20)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4..20)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2956..2967
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(3722..3756)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     3792..3808
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4160..5812
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4226..4244)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tgtgtcatag ctgtttcctg caaagaatgg tgatgacaat tgtacaggtt atcaataaag
       61 ccattgatac tctcattctt atcccgtatt tattaacttc tcctagtatt agtaattatc
      121 atggctgtca tggcgcatta acggaataaa gggtgtgctt aaatcgggcc attttgcgta
      181 ataagaaaaa ggattaatta tgagcgaatt gaattaataa taaggtaata gatttacatt
      241 agaaaatgaa aggggatttt atgcgtgaga atgttacagt ctatcccggc aatagttacc
      301 cttattatca agataagaaa gaaaaggatt tttcgctacg ctcaaatcct ttaaaaaaac
      361 acaaaagacc acatttttta atgtggtctt ttattcttca actaaagcac ccattagttc
      421 aacaaacgaa aattggataa agtgggatat ttttaaaata tatatttatg ttacagtaat
      481 attgactttt aaaaaaggat tgattataat gaagaaagca gacaagtaag cctcctaaat
      541 tcactttaga taaaaattta ggaggcatat caaatgaact ttaataaaat tcactttaga
      601 taaaaattta ggaggcatat caaatgaact ttaataaaat tgatttagac aattggaaga
      661 gaaaagagat atttaatcat tatttgaacc aacaaacgac ttttagtata accacagaaa
      721 ttgatattag tgttttatac cgaaacataa aacaagaagg atataaattt taccctgcat
      781 ttattttctt agtgacaagg gtgataaact caaatacagc ttttagaact ggttacaata
      841 gcgacggaga gttaggttat tgggataagt tagagccact ttatacaatt tttgatggtg
      901 tatctaaaac attctctggt atttggactc ctgtaaagaa tgacttcaaa gagttttatg
      961 atttatacct ttctgatgta gagaaatata atggttcggg gaaattgttt cccaaaacac
     1021 ctatacctga aaatgctttt tctctttcta ttattccatg gacttcattt actgggttta
     1081 acttaaatat caataataat agtaattacc ttctacccat tattacagca ggaaaattca
     1141 ttaataaagg taattcaata tatttaccgc tatctttaca ggtacatcat tctgtttgtg
     1201 atggttatca tgcaggattg tttatgaact ctattcagga attgtcagat aggcctaatg
     1261 actggctttt ataatatgag ataatgccga ctgtactttt tacagtcggt tttctaatgt
     1321 cactaacctg ccccgttagt tgaagaaggt ttttatatta cagctccaga tccatatcct
     1381 tctttttctg aaccgacttc tcctttttcg cttctttatt ccaattgctt tattgacgtt
     1441 gagcctcgga acccttaaca atcccaaaac ttgtcgactt tcgtcagggg ggcttttatt
     1501 tattcaataa tccctcctct caataaatct attgttgtac ttaattcaac ttccatttct
     1561 ctgtatcttt caatacgctc ttttaagtcc ttaatttctt tttttaattc ctcattttca
     1621 gcaaataact ctttttcttt gtttgtcatt ttatttcccc cgtttcagca tcaagaacct
     1681 ttgcataact tgctctatat ccacactgat aattgccctc aaaccataat ctaaaggcgc
     1741 tagagtttgt tgaaacaata tcttttacat cattcgtatt taaaattcca aactccgctc
     1801 ccctaaggcg aataaaagcc attaactctt ttgtatttac caaattatag tcatccacta
     1861 tatctaaaag taaattcttc aattttcttt tttggctttc atcaagtgtt atatagcggt
     1921 caatatcaaa atcattaatg ttcaaaatat cttttttgtc gtatatatgt ttattcttag
     1981 caatagcgtc ctttgattca tgagtcaaat attcatatga acctttgata taatcaagta
     2041 tctcaacatg agcaactgaa ctattcccca attttcgctt aatcttgttc ctaacgcttt
     2101 ctattgttac aggatttcgt gcaatatata taacgtgata gtgtggtttt ttatagtgct
     2161 ttccatttcg tataacatca ctactattcc atgtatcttt atcttttttt tcgtccatat
     2221 cgtgtaaagg actgacagcc atagatacgc ccaaactctc taatttttct ttccaatcat
     2281 taggaattga gtcaggatat aataaaaatc caaaatttct agctttagta tttttaatag
     2341 ccatgatata attaccttat caaaaacaag tagcgaaaac tcgtatcctt ctaaaaacgc
     2401 gagctttcgc ttattttttt tgttctgatt cctttcttgc atattcttct atagctaacg
     2461 ccgcaaccgc agattttgaa aaaccttttt gtttcgccat atctgttaat tttttatctt
     2521 gctcttttgt cagagaaatc ataactcttt ttttcgattc tgaaatcacc atttaaaaaa
     2581 ctccaatcaa ataattttat aaaattagtg tatcactttg taatcataaa aacaacaata
     2641 aagctactta aatatagatt tataaaaaac gttggcgaaa acgttggcga ttcgttggcg
     2701 attgaaaaac ccctcaaacc cttgagccag ttgggataga gcgtttttgg cacaaaaatt
     2761 tgcactcggc acttaatggg gggtcgtagt acggaagcaa aattcgcttc ctttcccccc
     2821 atttttttcc aaattccaaa tttttttcaa aaattttcca gcgctaccgc tcggcaaaat
     2881 tgcaagcaat ttttaaaatc aaacccatga gggaatttca ttccctcaaa ctcccttgag
     2941 cctcctccaa ccgaaataga aggacgctgc gcttattatt tcattcagtc atcggctttc
     3001 ataatctaac agacaacatc ttcgctgcaa agccacgcta cgctcaaggg cttttacgct
     3061 acgataacgc ctgttttaac gattatgccg ataactaaac gaaataaacg ctaaaacgtc
     3121 acagaaacga ttttcagacg ttttaataaa aaatcgaccg aaaaataaat ataaatcgga
     3181 tatacaatcg gcaattgacg aaactgcaaa atatcctgta aaggatacgg attttatgac
     3241 cgatgatgaa gaaaagaatt tgaaacgttt gtctgatttg gaggaaggtt tacaccgtaa
     3301 aaggttaatc tcctatggtg gtttgttaaa agaaatacat aaaaaattaa accttgatga
     3361 cacagaagaa ggcgatttga ttcatacaga tgatgacgaa aaagccgatg aagatggatt
     3421 ttctattatt gcaatgtgga attgggaacg gaaaaattat tttattaaag agtagttcaa
     3481 caaacgggcc agtttgttga agattagatg ctataattgt tattaaaagg attgaaggat
     3541 gcttaggaag acgagttatt aatagctgaa taagaacggt gctctccaaa tattcttatt
     3601 tagaaaagca aatctaaaat tatctgaaaa gggaatgaga atagtgaatg gaccaataat
     3661 aatgactaga gaagaaagaa tgaagattgt tcatgaaatt aaggaacgaa tattggataa
     3721 cataaaaaac gcccggcggc aaccgagcgt tctgaattat ggggatgatg ttaaggctat
     3781 tggtgtttat ggtaaaacga cggccagtca gaattcaaaa aaaaatctag atttttgtgt
     3841 tgtttagttg cagtcatttc taaacatctc cttaatttta ttagagcaaa agctcatacg
     3901 gtcttatttt aacatattga taattgtttg tcattgaatt cagaaaaata atgaaaatag
     3961 tttactgata acgcttccaa taagaaaaaa taaggaattt atggtataat gaaatctagt
     4021 attgactaga atactagtac tgcagtgaaa ttcaaaaaaa aatctagaag aattaaatta
     4081 aaaagggagg ccaaatataa tgaaaaatat gaatgacaat gatgttccgc ggtggcggcg
     4141 atctaaggag gaataaaaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt
     4201 ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
     4261 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc
     4321 ggaatacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
     4381 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
     4441 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
     4501 gctcaacagt atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca
     4561 aaaaattttg aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc
     4621 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
     4681 cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact
     4741 gataatgaat tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac
     4801 tgcctgcgtc agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
     4861 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
     4921 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt
     4981 acgatccctt caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt
     5041 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     5101 tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc
     5161 agggatacga caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     5221 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     5281 ggatctggat accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg
     5341 acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     5401 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     5461 agttgaccgc ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt
     5521 ggaatcgata ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga
     5581 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     5641 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     5701 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     5761 aatcagagag atcctcataa aggccaagaa gggcggaaag tccaaattgt aatactagta
     5821 ctgcag
//
