LOCUS       sequence-444107-        3895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the protein consisting of 35 amino acid of Rotavirus SA11 
            NSP1 protein and the full-length SA11 NSP5 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3895)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-35aaNSP1SA11-NSP5(wt)
FEATURES             Location/Qualifiers
     source          1..3895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(93..161,162..953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(311..330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1124..1712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1613..1632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1866..1883
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1968..1986
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2146..2157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2952..2970)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2966..3013
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(3081..3099)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3082..3099)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3389..3408)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3618..3637)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3737..3759
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3797..3815)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(3883..3895,1..92)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
       61 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      121 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      181 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      241 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      301 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      361 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      421 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      481 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
      541 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
      601 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
      661 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
      721 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
      781 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
      841 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
      901 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
      961 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1021 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1141 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1201 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1261 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1321 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1381 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1441 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1501 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1561 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1621 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1681 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1741 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1801 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     1861 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     1921 ccgcgcgttg gccgattcat taatgcaggg ggatatcgat cccgggttaa tacgactcac
     1981 tataggcttt tttttgaaaa gtcttgtgtt agccatggct acttttaaag atgcatgctt
     2041 tcattatcgt agattaactg ctttaaatcg gagattatgc aacattggtg caaattctat
     2101 ttggatgcca gttcctgata tgtctctcag tattgacgtg acgagtcttc cttctattcc
     2161 ttcaactata tataagaatg aatcgtcttc aacaacgtca actctttctg gaaaatctat
     2221 tggtaggagt gaacagtaca tttcaccaga tgcagaagca ttcaataaat acatgctgtc
     2281 gaagtctcca gaggatattg gaccatctga ttctgcttca aacgatccac tcaccagttt
     2341 ttcgattaga tcgaatgcag ttaagacaaa cgcagacgct ggcgtgtcta tggattcatc
     2401 agcacaatca cgaccttcaa gtaatgtcgg atgcgatcaa gtggatttct ccttaaataa
     2461 aggcttaaaa gtaaaagcta atttggactc atcaatatca atatctacgg atactaaaaa
     2521 ggagaaatca aaacaaaacc ataaaagtag gaagcactac ccaagaattg aagcagagtc
     2581 tgattcagat gattatgtac tggatgattc agatagtgat gatggtaaat gtaagaactg
     2641 taaatataag aagaaatact tcgcattaag aatgagaatg aaacaagtcg caatgcaatt
     2701 gattgaagat ttgtaaaatt atgtcactat ctaattatac agtatttagc catcacaaga
     2761 ccgtccagac tagagtagcg cctagctggc aaaatactgt gaaccgggtc ggcatggcat
     2821 ctccacctcc tcgcggtccg acctgggcat ccgaaggagg acgtcgtcca ctcggatggc
     2881 taagggagag ctcaaaaaaa aggatccggc tgctaacaaa gcccgaaagg aagctgagtt
     2941 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     3001 gaggggtttt ttgctgaaag gaggaactat atccggatcg agatcctcta gagtcgacct
     3061 gcaggcatgc aagcttgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     3121 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     3181 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     3241 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     3301 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     3361 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     3421 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     3481 gtggccgggg gactgttggg cgccatctcc ttgcaccatt ccttgcggcg gcggtgctca
     3541 acggcctcaa cctactactg ggctgcttct taatgcagga gtcgcataag ggagagcgtc
     3601 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     3661 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     3721 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3781 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3841 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac cccta
//
