LOCUS       sequence-444108-        3740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the short NSP5 protein (17 amino acids) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3740)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP5(Y18Stop)SA11
FEATURES             Location/Qualifiers
     source          1..3740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(76..87)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(842..860)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      856..903
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(971..989)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(972..989)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1279..1298)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1508..1527)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1627..1649
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1687..1705)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1773..1877
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1878..1946,1947..2738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2096..2115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2909..3497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3398..3417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3651..3668
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcgatcccgg ttaatacgac tcactatagg cttttaaagc gctacagtga tgtctctcag
       61 tattgacgtg acgagtcttc cttctattcc ttcaactata tagaagaatg aatcgtcttc
      121 aacaacgtca actctttctg gaaaatctat tggtaggagt gaacagtaca tttcaccaga
      181 tgcagaagca ttcaataaat acatgctgtc gaagtctcca gaggatattg gaccatctga
      241 ttctgcttca aacgatccac tcaccagttt ttcgattaga tcgaatgcag ttaagacaaa
      301 cgcagacgct ggcgtgtcta tggattcatc agcacaatca cgaccttcaa gtaatgtcgg
      361 atgcgatcaa gtggatttct ccttaaataa aggcttaaaa gtaaaagcta atttggactc
      421 atcaatatca atatctacgg atactaaaaa ggagaaatca aaacaaaacc ataaaagtag
      481 gaagcactac ccaagaattg aagcagagtc tgattcagat gattatgtac tggatgattc
      541 agatagtgat gatggtaaat gtaagaactg taaatataag aagaaatact tcgcattaag
      601 aatgagaatg aaacaagtcg caatgcaatt gattgaagat ttgtaagtct gacctgggaa
      661 cacactaggg agctccccac tcccgttttg tgaccgggtc ggcatggcat ctccacctcc
      721 tcgcggtccg acctgggcat ccgaaggagg acgtcgtcca ctcggatggc taagggagag
      781 ctcaaaaaaa aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      841 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      901 ttgctgaaag gaggaactat atccggatcg agatcctcta gagtcgacct gcaggcatgc
      961 aagcttgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     1021 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
     1081 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
     1141 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
     1201 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     1261 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     1321 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     1381 gactgttggg cgccatctcc ttgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     1441 cctactactg ggctgcttct taatgcagga gtcgcataag ggagagcgtc gaatggtgca
     1501 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     1561 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     1621 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     1681 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1741 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1801 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1861 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1921 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1981 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2041 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2101 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2161 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2221 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2281 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2341 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2401 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2461 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2521 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2581 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2641 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2701 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2761 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2821 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2881 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2941 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3001 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3061 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3121 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3181 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3241 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3301 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3361 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3421 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3481 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3541 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3601 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3661 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cattggccga
     3721 ttcattaatg cagggggata
//
