LOCUS       sequence-444148-       10438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human RNF149 with 3x FLAG tag in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Kim J, de Haro M, Al-Ramahi I, Garaicoechea LL, Jeong HH, Sonn JY, 
            Tadros B, Liu Z, Botas J, Zoghbi HY
  TITLE     Evolutionarily conserved regulators of tau identify targets for new 
            therapies.
  JOURNAL   Neuron. 2023 Mar 15;111(6):824-838.e7. doi: 
            10.1016/j.neuron.2022.12.012. Epub 2023 Jan 6.
  PUBMED    36610398
REFERENCE   2  (bases 1 to 10438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2022.12.012"; journalName: "Neuron"; date: 
            "2023-03-15- 15"; volume: "111"; issue: "6"; pages: "824-838.e7"
COMMENT     Sequence Label: Human RNF149-3X FLAG
FEATURES             Location/Qualifiers
     source          1..10438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1184..1318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1221..1240)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1275..1294
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1486..2119
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2166..2291
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2788..3021
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3206..3250
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3513..3628
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        3686..3989
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3990..4193
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4144..4164
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4190..4214
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4348..4372
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5579..5603)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5611..5676
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5696..6268
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5863..5880)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6090..6109
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6274..6294
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6295..6987
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="ESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMK
                     STKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLH
                     VSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRT
                     FSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAFK
                     TPDADAGEE"
     misc_feature    7537..8125
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7590..7610)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8008..8019)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8332..8565
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8666..8683)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8672..8896
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8942..9370
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9029..9048)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9239..9260
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9379..9399)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9384..9713
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9564..9699
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9626..9645
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     rep_origin      complement(9850..10438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9930..9949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
     1201 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1261 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     1321 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     1381 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     1441 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     1501 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     1561 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     1621 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     1681 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     1741 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     1801 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     1861 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     1921 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     1981 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     2041 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     2101 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     2161 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     2221 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     2281 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     2341 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     2401 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     2461 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     2521 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     2581 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     2641 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     2701 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     2761 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     2821 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     2881 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     2941 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     3001 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     3061 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     3121 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     3181 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     3241 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     3301 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     3361 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     3421 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     3481 tcgattagtg aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt
     3541 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     3601 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     3661 tttatcgata agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     3721 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     3781 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     3841 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     3901 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     3961 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     4021 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4081 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     4141 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     4201 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     4261 gactctacta gattaattaa gtttaaactt aagcttggta ccgagctcgg atccactagt
     4321 ccagtgtggt ggaattctgc agatatcaca agtttgtaca aaaaagctgg caccatggcg
     4381 tggcggcggc gcgaagccag cgtcggggct cgcggcgtgt tggctctggc gttgctcgcc
     4441 ctggccctgt gcgtgcccgg ggcccggggc cgggctctcg agtggttctc ggccgtggta
     4501 aacatcgagt acgtggaccc gcagaccaac ctgacggtgt ggagcgtctc ggagagtggc
     4561 cgcttcggcg acagctcgcc caaggagggc gcgcatggcc tggtgggcgt cccgtgggcg
     4621 cccggcggag acctcgaggg ctgcgcgccc gacacgcgct tcttcgtgcc cgagcccggc
     4681 ggccgagggg ccgcgccctg ggtcgccctg gtggctcgtg ggggctgcac cttcaaggac
     4741 aaggtgctgg tggcggcgcg gaggaacgcc tcggccgtcg tcctctacaa tgaggagcgc
     4801 tacgggaaca tcaccttgcc catgtctcac gcgggaacag gaaatatagt ggtcattatg
     4861 attagctatc caaaaggaag agaaattttg gagctggtgc aaaaaggaat tccagtaacg
     4921 atgaccatag gggttggcac ccggcatgta caggagttca tcagcggtca gtctgtggtg
     4981 tttgtggcca ttgccttcat caccatgatg attatctcgt tagcctggct aatattttac
     5041 tatatacagc gtttcctata tactggctct cagattggaa gtcagagcca tagaaaagaa
     5101 actaagaaag ttattggcca gcttctactt catactgtaa agcatggaga aaagggaatt
     5161 gatgttgatg ctgaaaattg tgcagtgtgt attgaaaatt tcaaagtaaa ggatattatt
     5221 agaattctgc catgcaagca tatttttcat agaatatgca ttgacccatg gcttttggat
     5281 caccgaacat gtccaatgtg taaacttgat gtcatcaaag ccctaggata ttggggagag
     5341 cctggggatg tacaggagat gcctgctcca gaatctcctc ctggaaggga tccagctgca
     5401 aatttgagtc tagctttacc agatgatgac ggaagtgatg agagcagtcc accatcagcc
     5461 tcccctgctg aatctgagcc acagtgtgat cccagcttta aaggagatgc aggagaaaat
     5521 acggcattgc tagaagccgg caggagtgac tctcggcatg gcggacccat ctccggcgac
     5581 ccagctttct tgtacaaagt ggttgctagc gactacaaag accatgacgg tgattataaa
     5641 gatcatgaca tcgattacaa ggatgacgat gacaagtaat gaaccgggcg cgccgcccct
     5701 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5761 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5821 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5881 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5941 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     6001 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     6061 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     6121 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     6181 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     6241 tggttttcct ttgaaaaaca cgataatacc atgcccaaga agaagagaaa ggtggagagc
     6301 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     6361 gtggagttcg agctggtggg cggcggagag ggcacccccg agcagggccg catgaccaac
     6421 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     6481 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     6541 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     6601 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     6661 atgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     6721 gccaccgtgg agcacctgca ccccatgggc gataacgatc tggatggcag cttcacccgc
     6781 accttcagcc tgcgcgacgg cggctactac agctccgtgg tggacagcca catgcacttc
     6841 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     6901 gtggaggagg atcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     6961 accccggatg cagatgccgg tgaagaaaga tctcgagctc aagcttcgaa ttctgcagtc
     7021 gagcagctgt tgaattttga ccttcttaag cttgcgggag acgtcgagtc caaccctggg
     7081 cccggtacca ggagggccac catgaagacc ttcaacatct ctcagcagga tctggagctg
     7141 gtggaggtcg ccactgagaa gatcaccatg ctctatgagg acaacaagca ccatgtcggg
     7201 gcggccatca ggaccaagac tggggagatc atctctgctg tccacattga ggcctacatt
     7261 ggcagggtca ctgtctgtgc tgaagccatt gccattgggt ctgctgtgag caacgggcag
     7321 aaggactttg acaccattgt ggctgtcagg cacccctact ctgatgaggt ggacagatcc
     7381 atcagggtgg tcagcccctg tggcatgtgc agagagctga tctctgacta tgctcctgac
     7441 tgctttgtgc tcattgagat gaatggcaag ctggtcaaaa ccaccattga ggaactcatc
     7501 cccctcaagt acaccaggaa ctaataataa gttaacaatc aacctctgga ttacaaaatt
     7561 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7621 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7681 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7741 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7801 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7861 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7921 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7981 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8041 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8101 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     8161 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     8221 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     8281 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     8341 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8401 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8461 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8521 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     8581 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     8641 gacattgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     8701 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     8761 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     8821 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     8881 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     8941 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9001 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9061 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9121 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9181 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9241 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9301 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9361 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     9421 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     9481 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     9541 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     9601 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     9661 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     9721 cgggagcttg tatatccatt ttcggatctg atcagcacgt gatgaaaaag cctgaactca
     9781 ccgcgacgtc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10261 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaa
//
