LOCUS       sequence-444151-       12048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human mutant USP7 with 3x FLAG tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12048)
  AUTHORS   Kim J, de Haro M, Al-Ramahi I, Garaicoechea LL, Jeong HH, Sonn JY, 
            Tadros B, Liu Z, Botas J, Zoghbi HY
  TITLE     Evolutionarily conserved regulators of tau identify targets for new 
            therapies.
  JOURNAL   Neuron. 2023 Mar 15;111(6):824-838.e7. doi: 
            10.1016/j.neuron.2022.12.012. Epub 2023 Jan 6.
  PUBMED    36610398
REFERENCE   2  (bases 1 to 12048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2022.12.012"; journalName: "Neuron"; date: 
            "2023-03-15- 15"; volume: "111"; issue: "6"; pages: "824-838.e7"
COMMENT     Sequence Label: Human USP7_H456A-3X FLAG
FEATURES             Location/Qualifiers
     source          1..12048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3390..3413
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6842..7391
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6986..7003)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7213..7232
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7394..7993
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7394..7413)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7890..7910
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8017..8605
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8070..8090)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8488..8499)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8812..9445
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9573..9589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9573..9589)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9586..9608)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9597..9613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9621..9627,9628..9645,9646..9651))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9666..9687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9804..9821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9975..10563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10055..10074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10734..11525,11526..11594))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11357..11376
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11595..11699)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11747..11881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11784..11803)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11838..11857
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccatgg attacaagga tgacgatgac aagaccggta
     3421 tgaaccacca gcagcagcag cagcagcaga aagcgggcga gcagcagttg agcgagcccg
     3481 aggacatgga gatggaagcg ggagatacag atgacccacc aagaattact cagaaccctg
     3541 tgatcaatgg gaatgtggcc ctgagtgatg gacacaacac cgcggaggag gacatggagg
     3601 atgacaccag ttggcgctcc gaggcaacct ttcagttcac tgtggagcgc ttcagcagac
     3661 tgagtgagtc ggtccttagc cctccgtgtt ttgtgcgaaa tctgccatgg aagattatgg
     3721 tgatgccacg cttttatcca gacagaccac accaaaaaag cgtaggattc tttctccagt
     3781 gcaatgctga atctgattcc acgtcatggt cttgccatgc acaagcagtg ctgaagataa
     3841 taaattacag agatgatgaa aagtcgttca gtcgtcgtat tagtcatttg ttcttccata
     3901 aagaaaatga ttggggattt tccaatttta tggcctggag tgaagtgacc gatcctgaga
     3961 aaggatttat agatgatgac aaagttacct ttgaagtctt tgtacaggcg gatgctcccc
     4021 atggagttgc gtgggattca aagaagcaca caggctacgt cggcttaaag aatcagggag
     4081 cgacttgtta catgaacagc ctgctacaga cgttattttt cacgaatcag ctacgaaagg
     4141 ctgtgtacat gatgccaacc gagggggatg attcgtctaa aagcgtccct ttagcattac
     4201 aaagagtgtt ctatgaatta cagcatagtg ataaacctgt aggaacaaaa aagttaacaa
     4261 agtcatttgg gtgggaaact ttagatagct tcatgcaaca tgatgttcag gagctttgtc
     4321 gagtgttgct cgataatgtg gaaaataaga tgaaaggcac ctgtgtagag ggcaccatac
     4381 ccaaattatt ccgcggcaaa atggtgtcct atatccagtg taaagaagta gactatcggt
     4441 ctgatagaag agaagattat tatgatatcc agctaagtat caaaggaaag aaaaatatat
     4501 ttgaatcatt tgtggattat gtggcagtag aacagctcga tggggacaat aaatacgacg
     4561 ctggggaaca tggcttacag gaagcagaga aaggtgtgaa attcctaaca ttgccaccag
     4621 tgttacatct acaactgatg agatttatgt atgaccctca gacggaccaa aatatcaaga
     4681 tcaatgatag gtttgaattc ccagagcagt taccacttga tgaatttttg caaaaaacag
     4741 atcctaagga ccctgcaaat tatattcttc atgcagtcct ggttgctagt ggagataatc
     4801 atggtggaca ttatgtggtt tatctaaacc ccaaagggga tggcaaatgg tgtaaatttg
     4861 atgacgacgt ggtgtcaagg tgtactaaag aggaagcaat tgagcacaat tatgggggtc
     4921 acgatgacga cctgtctgtt cgacactgca ctaatgctta catgttagtc tacatcaggg
     4981 aatcaaaact gagtgaagtt ttacaggcgg tcaccgacca tgatattcct cagcagttgg
     5041 tggagcgatt acaagaagag aaaaggatcg aggctcagaa gcggaaggag cggcaggaag
     5101 cccatctcta tatgcaagtg cagatagtcg cagaggacca gttttgtggc caccaaggga
     5161 atgacatgta cgatgaagaa aaagtgaaat acactgtgtt taaagtattg aagaactcct
     5221 cgcttgctga gtttgttcag agcctctctc agaccatggg atttccacaa gatcaaattc
     5281 gattgtggcc catgcaagca aggagtaatg gaacaaaacg accagcaatg ttagataatg
     5341 aagccgacgg caataaaaca atgattgagc tcagtgataa tgaaaaccct tggacaatat
     5401 tcctggaaac agttgatccc gagctggctg ctagtggagc gaccttaccc aagtttgata
     5461 aagatcatga tgtaatgtta tttttgaaga tgtatgatcc caaaacgcgg agcttgaatt
     5521 actgtgggca tatctacaca ccaatatcct gtaaaatacg tgacttgctc ccagttatgt
     5581 gtgacagagc aggatttatt caagatacta gccttatcct ctatgaggaa gttaaaccga
     5641 atttaacaga gagaattcag gactatgacg tgtctcttga taaagccctt gatgaactaa
     5701 tggatggtga catcatagta tttcagaagg atgaccctga aaatgataac agtgaattac
     5761 ccaccgcaaa ggagtatttc cgagatctct accaccgcgt tgatgtcatt ttctgtgata
     5821 aaacaatccc taatgatcct ggatttgtgg ttacgttatc aaatagaatg aattattttc
     5881 aggttgcaaa gacagttgca cagaggctca acacagatcc aatgttgctg cagtttttca
     5941 agtctcaagg ttatagggat ggcccaggta atcctcttag acataattat gaaggtactt
     6001 taagagatct tctacagttc ttcaagccta gacaacctaa gaaactttac tatcagcagc
     6061 ttaagatgaa aatcacagac tttgagaaca ggcgaagttt taaatgtata tggttaaaca
     6121 gccaatttag ggaagaggaa ataacactat atccagacaa gcatgggtgt gtccgggacc
     6181 tgttagaaga atgtaaaaag gccgtggagc ttggggagaa agcatcaggg aaacttaggc
     6241 tgctagaaat tgtaagctac aaaatcattg gtgttcatca agaagatgaa ctattagaat
     6301 gtttatctcc tgcaacgagc cggacgtttc gaatagagga aatccctttg gaccaggtgg
     6361 acatagacaa agagaatgag atgcttgtca cagtggcgca tttccacaaa gaggtcttcg
     6421 gaacgttcgg aatcccgttt ttgctgagga tacaccaggg cgagcatttt cgagaagtga
     6481 tgaagcgaat ccagagcctg ctggacatcc aggagaagga gtttgagaag tttaaatttg
     6541 caattgtaat gatgggccga caccagtaca taaatgaaga cgagtatgaa gtaaatttga
     6601 aagactttga gccacagccc ggtaatatgt ctcatcctcg gccttggcta gggctcgacc
     6661 acttcaacaa agccccaaag aggagtcgct acacttacct tgaaaaggcc attaaaatcc
     6721 ataactgaga attcctcgag ggcggccgct ctagagtcga cgggccgcgg taacaattgt
     6781 taactaactt aagctagcaa cggtttccct ctagcgggat caattccgcc ccccccccct
     6841 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     6901 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     6961 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     7021 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     7081 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     7141 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     7201 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     7261 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     7321 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     7381 acacgataat accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     7441 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     7501 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     7561 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     7621 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     7681 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     7741 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     7801 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     7861 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     7921 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     7981 gcccggtgcc tgacccgggc ggggcgcgtc tggaacaatc aacctctgga ttacaaaatt
     8041 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8101 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8161 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     8221 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8281 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8341 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     8401 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     8461 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8521 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8581 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     8641 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     8701 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     8761 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     8821 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     8881 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     8941 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     9001 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     9061 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     9121 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     9181 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     9241 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     9301 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9361 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     9421 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     9481 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg
     9541 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9601 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9661 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9721 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9781 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9841 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9901 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9961 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    10021 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10081 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10141 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10201 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10261 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10321 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10381 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    10441 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10501 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10561 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10621 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10681 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10741 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10801 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10861 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10921 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10981 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    11041 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    11101 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    11161 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    11221 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    11281 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    11341 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    11401 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    11461 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11521 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    11581 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11641 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11701 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
    11761 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    11821 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    11881 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    11941 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    12001 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
