LOCUS       sequence-444152-       10405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human RNF130 with 3x FLAG tag in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Kim J, de Haro M, Al-Ramahi I, Garaicoechea LL, Jeong HH, Sonn JY, 
            Tadros B, Liu Z, Botas J, Zoghbi HY
  TITLE     Evolutionarily conserved regulators of tau identify targets for new 
            therapies.
  JOURNAL   Neuron. 2023 Mar 15;111(6):824-838.e7. doi: 
            10.1016/j.neuron.2022.12.012. Epub 2023 Jan 6.
  PUBMED    36610398
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2022.12.012"; journalName: "Neuron"; date: 
            "2023-03-15- 15"; volume: "111"; issue: "6"; pages: "824-838.e7"
COMMENT     Sequence Label: Human RNF130-3X FLAG
FEATURES             Location/Qualifiers
     source          1..10405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1184..1318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1221..1240)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1275..1294
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1486..2119
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2166..2291
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2788..3021
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3206..3250
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3513..3628
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        3686..3989
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3990..4193
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4144..4164
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4190..4214
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5578..5643
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5663..6235
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5830..5847)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6057..6076
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6241..6261
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6262..6954
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="ESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMK
                     STKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLH
                     VSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRT
                     FSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAFK
                     TPDADAGEE"
     misc_feature    7504..8092
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7557..7577)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7975..7986)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8299..8532
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8633..8650)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8639..8863
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8909..9337
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8996..9015)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9206..9227
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9346..9366)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9351..9680
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9531..9666
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9593..9612
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     rep_origin      complement(9817..10405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9897..9916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
     1201 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1261 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     1321 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     1381 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     1441 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     1501 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     1561 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     1621 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     1681 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     1741 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     1801 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     1861 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     1921 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     1981 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     2041 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     2101 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     2161 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     2221 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     2281 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     2341 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     2401 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     2461 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     2521 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     2581 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     2641 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     2701 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     2761 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     2821 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     2881 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     2941 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     3001 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     3061 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     3121 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     3181 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     3241 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     3301 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     3361 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     3421 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     3481 tcgattagtg aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt
     3541 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     3601 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     3661 tttatcgata agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     3721 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     3781 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     3841 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     3901 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     3961 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     4021 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4081 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     4141 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     4201 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     4261 gactctacta gattaattaa gtttaaactt aagcttggta ccgagctcgg atccatgagc
     4321 tgcgcggggc gggcgggccc tgcccggctc gccgcgctag ccctgctgac ctgcagcctg
     4381 tggccggcac gggcagacaa cgcgagccag gagtactaca cagcgctcat caacgtgacg
     4441 gtgcaggagc ccggccgcgg cgccccgctc acgtttcgca tcgaccgcgg gcgctacggg
     4501 cttgactccc ccaaggccga ggtccgcggc caggtgctgg cgccgctgcc cctccacgga
     4561 gttgctgatc atctgggctg tgatccacaa acccggttct ttgtccctcc taatatcaaa
     4621 cagtggattg ccttgctgca gaggggaaac tgcacgttta aagagaaaat atcacgggcc
     4681 gctttccaca atgcagttgc tgtagtcatc tacaataata aatccaaaga ggagccagtt
     4741 accatgactc atccaggcac tggagatatt attgctgtca tgataacaga attgaggggt
     4801 aaggatattt tgagttatct ggagaaaaac atctctgtac aaatgacaat agctgttgga
     4861 actcgaatgc caccgaagaa cttcagccgt ggctctctag tcttcgtgtc aatatccttt
     4921 attgttttga tgattatttc ttcagcatgg ctcatattct acttcattca gaagatcagg
     4981 tacacaaatg cacgcgacag gaaccagcgt cgtctcggag atgcagccaa gaaagccatc
     5041 agtaaattga caaccaggac agtaaagaag ggtgacaagg aaactgaccc agactttgat
     5101 cattgtgcag tctgcataga gagctataag cagaatgatg tcgtccgaat tctcccctgc
     5161 aagcatgttt tccacaaatc ctgcgtggat ccctggctta gtgaacattg tacctgtcct
     5221 atgtgcaaac ttaatatatt gaaggccctg ggaattgtgc cgaatttgcc atgtactgat
     5281 aacgtagcat tcgatatgga aaggctcacc agaacccaag ctgttaaccg aagatcagcc
     5341 ctcggcgacc tcgccggcga caactccctt ggccttgagc cacttcgaac ttcggggatc
     5401 tcacctcttc ctcaggatgg ggagctcact ccgagaacag gagaaattaa cattgcagta
     5461 acaaaagaat ggtttattat tgccagtttt ggcctcctca gtgccctcac actctgctac
     5521 atgatcatca gagccacagc tagcttgaat gctaatgagg tagaatggtt ttctagagac
     5581 tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga tgacgatgac
     5641 aagtaatgaa ccgggcgcgc cgcccctctc cctccccccc ccctaacgtt actggccgaa
     5701 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     5761 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     5821 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     5881 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     5941 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     6001 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     6061 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     6121 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     6181 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg
     6241 cccaagaaga agagaaaggt ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     6301 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     6361 acccccgagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     6421 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     6481 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     6541 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     6601 ggccgcgtga tcggcgactt caaggtgatg ggcaccggct tccccgagga cagcgtgatc
     6661 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     6721 aacgatctgg atggcagctt cacccgcacc ttcagcctgc gcgacggcgg ctactacagc
     6781 tccgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     6841 gggggcccca tgttcgcctt ccgccgcgtg gaggaggatc acagcaacac cgagctgggc
     6901 atcgtggagt accagcacgc cttcaagacc ccggatgcag atgccggtga agaaagatct
     6961 cgagctcaag cttcgaattc tgcagtcgag cagctgttga attttgacct tcttaagctt
     7021 gcgggagacg tcgagtccaa ccctgggccc ggtaccagga gggccaccat gaagaccttc
     7081 aacatctctc agcaggatct ggagctggtg gaggtcgcca ctgagaagat caccatgctc
     7141 tatgaggaca acaagcacca tgtcggggcg gccatcagga ccaagactgg ggagatcatc
     7201 tctgctgtcc acattgaggc ctacattggc agggtcactg tctgtgctga agccattgcc
     7261 attgggtctg ctgtgagcaa cgggcagaag gactttgaca ccattgtggc tgtcaggcac
     7321 ccctactctg atgaggtgga cagatccatc agggtggtca gcccctgtgg catgtgcaga
     7381 gagctgatct ctgactatgc tcctgactgc tttgtgctca ttgagatgaa tggcaagctg
     7441 gtcaaaacca ccattgagga actcatcccc ctcaagtaca ccaggaacta ataataagtt
     7501 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7561 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7621 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7681 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7741 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7801 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7861 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     7921 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7981 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8041 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     8101 aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     8161 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     8221 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8281 taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     8341 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8401 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8461 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8521 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8581 aatgaatatc agagagtgag aggccttgac attgtttaaa cccgctgatc agcctcgact
     8641 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8701 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8761 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8821 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8881 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     8941 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9001 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9061 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9121 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9181 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9241 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9301 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     9361 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9421 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9481 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9541 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9601 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9661 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     9721 agcacgtgat gaaaaagcct gaactcaccg cgacgtcatg tgagcaaaag gccagcaaaa
     9781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10261 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10321 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10381 tacgcgcaga aaaaaaggat ctcaa
//
