LOCUS       sequence-444156-        7860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7860)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Desmoglein1-N-18
FEATURES             Location/Qualifiers
     source          1..7860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        131..434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        435..638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     589..609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      670..679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3871..3880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3877..3879,3880..3882,3883..4596)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3922..3943)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4183..4202)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4530..4551
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4718..4839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4755..4774)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4809..4828
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4846..5301)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4983..5004)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5195..5214
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5328..5432
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5434..5791
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5434..5625
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5457..5477)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5642..5777
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5826..6620
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5826..6620
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5880..5899)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6490..6509
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6808..6827)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6852..6899
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7228..7816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7717..7736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
       61 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      121 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      181 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      361 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      421 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      481 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      541 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      601 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      661 gctagcgtcg ccaccatgga ctggagtttc ttcagagtag ttgcagtgct gttcattttt
      721 ctggtggtgg tagaagttaa cagtgagttc cgaatccagg taagagatta taacactaaa
      781 aatggcacca tcaaatggca ttcaatccga aggcagaaac gtgaatggat caagttcgca
      841 gcagcctgtc gtgaaggtga agacaactca aagaggaacc caatcgccaa aattcactca
      901 gattgtgctg caaaccagca agttacatac cgcatctctg gagtaggaat tgatcagcca
      961 ccatatggga tctttgtcat taatcagaaa actggtgaaa ttaatataac atccatagtt
     1021 gatcgagagg tcactccttt cttcattatc tactgccgag ctctgaactc aatgggccaa
     1081 gatttagaga ggcctctaga gctcagagtc agggttttgg atataaatga caaccctcca
     1141 gtgttttcaa tggctacatt tgcaggacaa atagaagaaa attctaatgc aaatacactg
     1201 gtgatgatac tcaatgctac tgacgcagat gaaccgaaca atttgaactc aaaaatagcc
     1261 ttcaagatta taagacaaga accttcagat tcaccaatgt ttattatcaa cagaaatact
     1321 ggagaaattc gaacgatgaa taattttcta gacagagagc aatacggcca gtatgctctt
     1381 gctgtaagag gctctgaccg agatggtggg gcagatggca tgtcagcgga atgtgagtgc
     1441 aacattaaaa tcctcgatgt caatgataat atcccttaca tggaacagtc ttcatatacc
     1501 atagaaattc aagaaaatac tctaaattca aatttgctcg aaattagagt aattgatttg
     1561 gatgaagagt tctcagctaa ctggatggca gtaattttct ttatctctgg aaatgaagga
     1621 aattggtttg agatagaaat gaatgaaaga acaaatgtgg gaattttaaa ggttgttaag
     1681 cccttagatt atgaagctat gcagagtctg caactcagta ttggtgtcag aaataaagct
     1741 gaatttcatc attcaattat gtctcaatat aaactgaaag catctgcaat ttctgtgact
     1801 gtgttaaatg taattgaagg cccagtgttt cgtccaggtt caaagacata tgttgtaact
     1861 ggtaatatgg gatcaaatga taaagtggga gactttgtag ctactgacct ggacacaggt
     1921 agaccttcaa cgactgttag gtatgtaatg ggaaataatc cagctgacct gcttgctgtt
     1981 gattcaagaa caggcaaact cactttgaaa aataaagtta ccaaggaaca gtacaatatg
     2041 ctcggaggaa aataccaagg aacgattctc tctatagatg ataatcttca aagaacttgc
     2101 actggtacaa ttaatattaa cattcaaagt tttggtaatg acgacaggac taatacagag
     2161 ccgaacacta aaattactac caatactggc agacaagaaa gtacttcttc cactaactat
     2221 gataccagca caacttctac tgactctagc caagtatatt cttctgaacc cggaaacgga
     2281 gccaaagatt tgttatcaga caatgtacat tttggtcctg ctggcattgg actcctcatc
     2341 atgggattct tggtcttagg attggtccca tttttgatga tctgttgtga ttgtggaggt
     2401 gctcctcgta gtgcagctgg ctttgagcct gttcccgaat gttcagatgg agcaattcat
     2461 tcatgggcag tagaaggacc acagcctgaa cccagggata taaccactgt cataccacaa
     2521 ataccacctg ataacgcaaa tataattgaa tgcattgaca actcaggagt ttatacaaat
     2581 gagtatggtg gcagagaaat gcaagacctg ggaggaggag agagaatgac aggatttgaa
     2641 ctaacagagg gagttaaaac ttcaggaatg cctgagatat gtcaagaata ctctggaaca
     2701 ttaagaagaa attctatgag ggaatgtaga gaaggaggtc tgaatatgaa tttcatggaa
     2761 agctacttct gtcagaaagc atatgcttac gcagatgaag atgaaggacg cccatctaat
     2821 gactgtttgc tcatatatga catcgaaggt gtaggttccc ctgctggctc tgtgggttgt
     2881 tgtagcttca ttggagaaga cctggatgac agcttcttgg ataccctggg acctaaattt
     2941 aagaagttgg cagacatcag cctaggaaaa gaatcatatc cagaccttga tccttcttgg
     3001 ccaccacaaa gcactgaacc agtttgcctt cctcaggaaa cagagcccgt tgttagtgga
     3061 cacccaccaa tctccccaca tttcggcact accacagtaa tttctgagag cacctatccc
     3121 tcgggacctg gtgtactgca tcctaagcct attctcgatc ctctgggcta tggtaatgtc
     3181 actgtgaccg agtcttacac cacctctgac actctgaagc cctctgtgca cgttcacgat
     3241 aaccgaccag catcaaacgt ggtagtgaca gagagagtgg tcggcccaat ctctggcgct
     3301 gatttgcatg gaatgttaga gatgcctgac ttgcgagatg ggtcgaatgt tatagtgaca
     3361 gaaagggtaa tagcaccaag ctctagtcta cccacctctc tgactatcca tcatcctaga
     3421 gagtcttcaa atgtggtagt gacagaaaga gtaatccaac caacttccgg catgataggt
     3481 agtctgagta tgcaccccga gttagccaat gcccacaatg tcattgtgac agagagggtt
     3541 gtttctggtg ctggcgtaac tggaattagt ggcaccactg ggatcagcgg tggcataggc
     3601 agcagtggcc tggttggcac cagcatgggt gctgggagcg gtgccctgag tggagctggc
     3661 ataagtggtg gtggcattgg cctgagcagc ttgggaggga cagccagcat tggccacatg
     3721 aggagttcct ctgaccatca ctttaaccaa accattgggt ccgcctcccc tagcacagct
     3781 cgaagtcgaa tcacaaagta tagtaccgtg caatatagca agagcagcgg tggagcaagc
     3841 gcagccagtg gtagcgcgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
     3901 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3961 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     4021 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc
     4081 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4141 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4201 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4261 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4321 cacaaggtct atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc
     4381 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     4441 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     4501 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4561 gggatcactc tcggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat
     4621 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     4681 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     4741 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4801 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     4861 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     4921 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     4981 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     5041 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     5101 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     5161 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     5221 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     5281 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5341 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5401 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     5461 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5521 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     5581 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     5641 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5701 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5761 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     5821 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     5881 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     5941 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     6001 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     6061 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6121 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     6181 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     6241 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     6301 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     6361 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     6421 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     6481 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     6541 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     6601 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     6661 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6721 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6781 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     6841 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     6901 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     6961 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     7021 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     7081 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7141 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7201 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7261 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7321 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7381 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7441 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7501 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7561 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7621 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7681 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7741 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7801 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
//
