LOCUS       sequence-444157-        7872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7872)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Desmoglein1-C-18
FEATURES             Location/Qualifiers
     source          1..7872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        63..366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        367..570
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     521..541
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      609..618
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(615..617,618..620,621..1331)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(660..681)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(921..940)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1268..1289
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4662..4783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4699..4718)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4753..4772
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4790..5245)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4927..4948)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5139..5158
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5272..5376
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5378..5735
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5378..5569
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5401..5421)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5586..5721
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5770..6564
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5770..6564
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5824..5843)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6434..6453
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6752..6771)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6796..6843
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7172..7760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7661..7680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      541 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      601 taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      661 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      721 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      781 tgccctggcc caccctcgtg accaccttga cctacggcgt gcagtgcttc gcccgctacc
      841 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      901 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      961 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1021 acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat atcaccgccg
     1081 acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc gaggacggca
     1141 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1201 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     1261 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1321 agctgtacaa gtccggactc agatctggca gcggtggagg cagcgcatcc ggcggaagcg
     1381 gaagcgactg gagtttcttc agagtagttg cagtgctgtt catttttctg gtggtggtag
     1441 aagttaacag tgagttccga atccaggtaa gagattataa cactaaaaat ggcaccatca
     1501 aatggcattc aatccgaagg cagaaacgtg aatggatcaa gttcgcagca gcctgtcgtg
     1561 aaggtgaaga caactcaaag aggaacccaa tcgccaaaat tcactcagat tgtgctgcaa
     1621 accagcaagt tacataccgc atctctggag taggaattga tcagccacca tatgggatct
     1681 ttgtcattaa tcagaaaact ggtgaaatta atataacatc catagttgat cgagaggtca
     1741 ctcctttctt cattatctac tgccgagctc tgaactcaat gggccaagat ttagagaggc
     1801 ctctagagct cagagtcagg gttttggata taaatgacaa ccctccagtg ttttcaatgg
     1861 ctacatttgc aggacaaata gaagaaaatt ctaatgcaaa tacactggtg atgatactca
     1921 atgctactga cgcagatgaa ccgaacaatt tgaactcaaa aatagccttc aagattataa
     1981 gacaagaacc ttcagattca ccaatgttta ttatcaacag aaatactgga gaaattcgaa
     2041 cgatgaataa ttttctagac agagagcaat acggccagta tgctcttgct gtaagaggct
     2101 ctgaccgaga tggtggggca gatggcatgt cagcggaatg tgagtgcaac attaaaatcc
     2161 tcgatgtcaa tgataatatc ccttacatgg aacagtcttc atataccata gaaattcaag
     2221 aaaatactct aaattcaaat ttgctcgaaa ttagagtaat tgatttggat gaagagttct
     2281 cagctaactg gatggcagta attttcttta tctctggaaa tgaaggaaat tggtttgaga
     2341 tagaaatgaa tgaaagaaca aatgtgggaa ttttaaaggt tgttaagccc ttagattatg
     2401 aagctatgca gagtctgcaa ctcagtattg gtgtcagaaa taaagctgaa tttcatcatt
     2461 caattatgtc tcaatataaa ctgaaagcat ctgcaatttc tgtgactgtg ttaaatgtaa
     2521 ttgaaggccc agtgtttcgt ccaggttcaa agacatatgt tgtaactggt aatatgggat
     2581 caaatgataa agtgggagac tttgtagcta ctgacctgga cacaggtaga ccttcaacga
     2641 ctgttaggta tgtaatggga aataatccag ctgacctgct tgctgttgat tcaagaacag
     2701 gcaaactcac tttgaaaaat aaagttacca aggaacagta caatatgctc ggaggaaaat
     2761 accaaggaac gattctctct atagatgata atcttcaaag aacttgcact ggtacaatta
     2821 atattaacat tcaaagtttt ggtaatgacg acaggactaa tacagagccg aacactaaaa
     2881 ttactaccaa tactggcaga caagaaagta cttcttccac taactatgat accagcacaa
     2941 cttctactga ctctagccaa gtatattctt ctgaacccgg aaacggagcc aaagatttgt
     3001 tatcagacaa tgtacatttt ggtcctgctg gcattggact cctcatcatg ggattcttgg
     3061 tcttaggatt ggtcccattt ttgatgatct gttgtgattg tggaggtgct cctcgtagtg
     3121 cagctggctt tgagcctgtt cccgaatgtt cagatggagc aattcattca tgggcagtag
     3181 aaggaccaca gcctgaaccc agggatataa ccactgtcat accacaaata ccacctgata
     3241 acgcaaatat aattgaatgc attgacaact caggagttta tacaaatgag tatggtggca
     3301 gagaaatgca agacctggga ggaggagaga gaatgacagg atttgaacta acagagggag
     3361 ttaaaacttc aggaatgcct gagatatgtc aagaatactc tggaacatta agaagaaatt
     3421 ctatgaggga atgtagagaa ggaggtctga atatgaattt catggaaagc tacttctgtc
     3481 agaaagcata tgcttacgca gatgaagatg aaggacgccc atctaatgac tgtttgctca
     3541 tatatgacat cgaaggtgta ggttcccctg ctggctctgt gggttgttgt agcttcattg
     3601 gagaagacct ggatgacagc ttcttggata ccctgggacc taaatttaag aagttggcag
     3661 acatcagcct aggaaaagaa tcatatccag accttgatcc ttcttggcca ccacaaagca
     3721 ctgaaccagt ttgccttcct caggaaacag agcccgttgt tagtggacac ccaccaatct
     3781 ccccacattt cggcactacc acagtaattt ctgagagcac ctatccctcg ggacctggtg
     3841 tactgcatcc taagcctatt ctcgatcctc tgggctatgg taatgtcact gtgaccgagt
     3901 cttacaccac ctctgacact ctgaagccct ctgtgcacgt tcacgataac cgaccagcat
     3961 caaacgtggt agtgacagag agagtggtcg gcccaatctc tggcgctgat ttgcatggaa
     4021 tgttagagat gcctgacttg cgagatgggt cgaatgttat agtgacagaa agggtaatag
     4081 caccaagctc tagtctaccc acctctctga ctatccatca tcctagagag tcttcaaatg
     4141 tggtagtgac agaaagagta atccaaccaa cttccggcat gataggtagt ctgagtatgc
     4201 accccgagtt agccaatgcc cacaatgtca ttgtgacaga gagggttgtt tctggtgctg
     4261 gcgtaactgg aattagtggc accactggga tcagcggtgg cataggcagc agtggcctgg
     4321 ttggcaccag catgggtgct gggagcggtg ccctgagtgg agctggcata agtggtggtg
     4381 gcattggcct gagcagcttg ggagggacag ccagcattgg ccacatgagg agttcctctg
     4441 accatcactt taaccaaacc attgggtccg cctcccctag cacagctcga agtcgaatca
     4501 caaagtatag taccgtgcaa tatagcaagt agggatccac cggatctaga taactgatca
     4561 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     4621 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     4681 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4741 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc
     4801 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     4861 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     4921 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4981 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     5041 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     5101 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     5161 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     5221 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5281 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5341 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
     5401 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5461 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5521 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5581 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5641 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5701 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga
     5761 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5821 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5881 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5941 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     6001 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6061 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     6121 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6181 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6241 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
     6301 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6361 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6421 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6481 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6541 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     6601 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6661 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6721 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     6781 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg
     6841 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     6901 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     6961 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact
     7021 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7081 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7141 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7201 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7261 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7321 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7381 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7441 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7501 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7561 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7621 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7681 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7741 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7801 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7861 ttaccgccat gc
//
