LOCUS       sequence-444171-        9201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9201)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 9201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Desmoglein1-C-18
FEATURES             Location/Qualifiers
     source          1..9201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(975..1682)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1250..1269)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1519..1537
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1679..1688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1727..1930)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1756..1776)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1931..2234
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2409..2997)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2489..2508)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3326..3373
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3398..3417
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3605..4399)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3605..4399)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3716..3735)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4326..4345
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4434..4791)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4448..4583
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4600..4791
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4748..4768
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(4793..4897)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4924..5379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5011..5030)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5221..5242
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5386..5507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5397..5416)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5451..5470
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aaaattactg ccatccagtt agctgagaac tcttcatcca aatcaattac tctaatttcg
       61 agcaaatttg aatttagagt attttcttga atttctatgg tatatgaaga ctgttccatg
      121 taagggatat tatcattgac atcgaggatt ttaatgttgc actcacattc cgctgacatg
      181 ccatctgccc caccatctcg gtcagagcct cttacagcaa gagcatactg gccgtattgc
      241 tctctgtcta gaaaattatt catcgttcga atttctccag tatttctgtt gataataaac
      301 attggtgaat ctgaaggttc ttgtcttata atcttgaagg ctatttttga gttcaaattg
      361 ttcggttcat ctgcgtcagt agcattgagt atcatcacca gtgtatttgc attagaattt
      421 tcttctattt gtcctgcaaa tgtagccatt gaaaacactg gagggttgtc atttatatcc
      481 aaaaccctga ctctgagctc tagaggcctc tctaaatctt ggcccattga gttcagagct
      541 cggcagtaga taatgaagaa aggagtgacc tctcgatcaa ctatggatgt tatattaatt
      601 tcaccagttt tctgattaat gacaaagatc ccatatggtg gctgatcaat tcctactcca
      661 gagatgcggt atgtaacttg ctggtttgca gcacaatctg agtgaatttt ggcgattggg
      721 ttcctctttg agttgtcttc accttcacga caggctgctg cgaacttgat ccattcacgt
      781 ttctgccttc ggattgaatg ccatttgatg gtgccatttt tagtgttata atctcttacc
      841 tggattcgga actcactgtt aacttctacc accaccagaa aaatgaacag cactgcaact
      901 actctgaaga aactccagtc gcttccgctt ccgccggatg cgctgcctcc accgctgcca
      961 gatctgagtc cggacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg
     1021 cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg
     1081 ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc
     1141 tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg
     1201 gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc
     1261 attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg
     1321 ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
     1381 acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg
     1441 gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg
     1501 aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
     1561 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc
     1621 accttgaagc gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc
     1681 atggtggcga ccggtagcgc tagcggatct gacggttcac taaaccagct ctgcttatat
     1741 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
     1801 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
     1861 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
     1921 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     1981 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     2041 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     2101 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     2161 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     2221 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     2281 actattaata actaatgcat ggcggtaata cggttatcca cagaatcagg ggataacgca
     2341 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2401 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2461 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2521 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2581 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2641 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2701 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2761 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2821 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2881 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2941 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3001 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3061 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3121 agttttaaat caatctaaag tatatatgag taacctgagg ctatggcagg gcctgccgcc
     3181 ccgacgttgg ctgcgagccc tgggccttca cccgaacttg gggggtgggg tggggaaaag
     3241 gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac agagtgccag
     3301 ccctgggacc gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt tttattctgt
     3361 ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg tgtttcagtt
     3421 agcctccccc tagggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
     3481 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
     3541 tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc
     3601 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     3661 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     3721 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     3781 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     3841 tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg
     3901 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     3961 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     4021 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     4081 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     4141 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     4201 gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     4261 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     4321 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     4381 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa
     4441 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
     4501 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     4561 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
     4621 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
     4681 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     4741 tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca ggactcttcc
     4801 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4861 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4921 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4981 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5041 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5101 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5161 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5221 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5281 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5341 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta cgcgttaaga tacattgatg
     5401 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     5461 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     5521 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     5581 acctctacaa atgtggtatg gctgattatg atcagttatc tagatccggt ggatccctac
     5641 ttgctatatt gcacggtact atactttgtg attcgacttc gagctgtgct aggggaggcg
     5701 gacccaatgg tttggttaaa gtgatggtca gaggaactcc tcatgtggcc aatgctggct
     5761 gtccctccca agctgctcag gccaatgcca ccaccactta tgccagctcc actcagggca
     5821 ccgctcccag cacccatgct ggtgccaacc aggccactgc tgcctatgcc accgctgatc
     5881 ccagtggtgc cactaattcc agttacgcca gcaccagaaa caaccctctc tgtcacaatg
     5941 acattgtggg cattggctaa ctcggggtgc atactcagac tacctatcat gccggaagtt
     6001 ggttggatta ctctttctgt cactaccaca tttgaagact ctctaggatg atggatagtc
     6061 agagaggtgg gtagactaga gcttggtgct attacccttt ctgtcactat aacattcgac
     6121 ccatctcgca agtcaggcat ctctaacatt ccatgcaaat cagcgccaga gattgggccg
     6181 accactctct ctgtcactac cacgtttgat gctggtcggt tatcgtgaac gtgcacagag
     6241 ggcttcagag tgtcagaggt ggtgtaagac tcggtcacag tgacattacc atagcccaga
     6301 ggatcgagaa taggcttagg atgcagtaca ccaggtcccg agggataggt gctctcagaa
     6361 attactgtgg tagtgccgaa atgtggggag attggtgggt gtccactaac aacgggctct
     6421 gtttcctgag gaaggcaaac tggttcagtg ctttgtggtg gccaagaagg atcaaggtct
     6481 ggatatgatt cttttcctag gctgatgtct gccaacttct taaatttagg tcccagggta
     6541 tccaagaagc tgtcatccag gtcttctcca atgaagctac aacaacccac agagccagca
     6601 ggggaaccta caccttcgat gtcatatatg agcaaacagt cattagatgg gcgtccttca
     6661 tcttcatctg cgtaagcata tgctttctga cagaagtagc tttccatgaa attcatattc
     6721 agacctcctt ctctacattc cctcatagaa tttcttctta atgttccaga gtattcttga
     6781 catatctcag gcattcctga agttttaact ccctctgtta gttcaaatcc tgtcattctc
     6841 tctcctcctc ccaggtcttg catttctctg ccaccatact catttgtata aactcctgag
     6901 ttgtcaatgc attcaattat atttgcgtta tcaggtggta tttgtggtat gacagtggtt
     6961 atatccctgg gttcaggctg tggtccttct actgcccatg aatgaattgc tccatctgaa
     7021 cattcgggaa caggctcaaa gccagctgca ctacgaggag cacctccaca atcacaacag
     7081 atcatcaaaa atgggaccaa tcctaagacc aagaatccca tgatgaggag tccaatgcca
     7141 gcaggaccaa aatgtacatt gtctgataac aaatctttgg ctccgtttcc gggttcagaa
     7201 gaatatactt ggctagagtc agtagaagtt gtgctggtat catagttagt ggaagaagta
     7261 ctttcttgtc tgccagtatt ggtagtaatt ttagtgttcg gctctgtatt agtcctgtcg
     7321 tcattaccaa aactttgaat gttaatatta attgtaccag tgcaagttct ttgaagatta
     7381 tcatctatag agagaatcgt tccttggtat tttcctccga gcatattgta ctgttccttg
     7441 gtaactttat ttttcaaagt gagtttgcct gttcttgaat caacagcaag caggtcagct
     7501 ggattatttc ccattacata cctaacagtc gttgaaggtc tacctgtgtc caggtcagta
     7561 gctacaaagt ctcccacttt atcatttgat cccatattac cagttacaac atatgtcttt
     7621 gaacctggac gaaacactgg gccttcaatt acatttaaca ctgagagatc ccctcataat
     7681 ttccccaaag cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt
     7741 aagtcttccc ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact
     7801 gttgagagta cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt
     7861 ttctgagcat gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc
     7921 atgatatcaa aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca
     7981 ggacttttca agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca
     8041 agttgtttgg gtgttcgaat ttcaacaggt aagttagttg ctagaatcca tggctccttt
     8101 gccgacgctg agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga
     8161 ttttttcggc ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt
     8221 ttagtcagcc tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg
     8281 ctgataggtt tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga
     8341 cttaaccagt accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca
     8401 tcactgacaa tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat
     8461 tggtcatgag ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta
     8521 acagaacgac cgtgtagtgc gactgaagct cgcaatacca taagccgttt ttgctcacgg
     8581 atatcagacc agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca
     8641 tgccaacggt atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg
     8701 attcgtttga tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta
     8761 agagtgagag ttttacagtc aagtaaggcg tggcaagcca acgttaagct gttgagtcgt
     8821 tttaagtgta attcggggca gaattggtaa agagagtcgt gtaaaatatc gagttcgcac
     8881 attttgttgt ctgattattg atttttggcg aaaccatttg atcatatgac aagatgtgta
     8941 tctaccttaa cttaatgatt ttgataaaaa tcattagggg attcatcagc atttaacaca
     9001 gtcacagaaa ttgcagatgc tttcagttta tattgagaca taattgaatg atgaaattca
     9061 gctttatttc tgacaccaat actgagttgc agactctgca tagcttcata atctaagggc
     9121 ttaacaacct ttaaaattcc cacatttgtt ctttcattca tttctatctc aaaccaattt
     9181 ccttcatttc cagagataaa g
//
