LOCUS       sequence-444173-        7851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7851)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Desmoglein1-N-18
FEATURES             Location/Qualifiers
     source          1..7851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        101..404
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        405..608
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     559..579
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      640..649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3841..3850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3847..4557
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4260..4279
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4679..4800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4716..4735)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4770..4789
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4807..5262)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4944..4965)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5156..5175
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5289..5393
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5395..5752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5395..5586
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5418..5438)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5603..5738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5787..6581
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5787..6581
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5841..5860)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6451..6470
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6769..6788)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6813..6860
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7189..7777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7678..7697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa
       61 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      121 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      181 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      241 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      301 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      361 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      421 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      481 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      541 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      601 gcagagctgg tttagtgaac cgtcagatcc gctagcgtcg ccaccatgga ctggagtttc
      661 ttcagagtag ttgcagtgct gttcattttt ctggtggtgg tagaagttaa cagtgagttc
      721 cgaatccagg taagagatta taacactaaa aatggcacca tcaaatggca ttcaatccga
      781 aggcagaaac gtgaatggat caagttcgca gcagcctgtc gtgaaggtga agacaactca
      841 aagaggaacc caatcgccaa aattcactca gattgtgctg caaaccagca agttacatac
      901 cgcatctctg gagtaggaat tgatcagcca ccatatggga tctttgtcat taatcagaaa
      961 actggtgaaa ttaatataac atccatagtt gatcgagagg tcactccttt cttcattatc
     1021 tactgccgag ctctgaactc aatgggccaa gatttagaga ggcctctaga gctcagagtc
     1081 agggttttgg atataaatga caaccctcca gtgttttcaa tggctacatt tgcaggacaa
     1141 atagaagaaa attctaatgc aaatacactg gtgatgatac tcaatgctac tgacgcagat
     1201 gaaccgaaca atttgaactc aaaaatagcc ttcaagatta taagacaaga accttcagat
     1261 tcaccaatgt ttattatcaa cagaaatact ggagaaattc gaacgatgaa taattttcta
     1321 gacagagagc aatacggcca gtatgctctt gctgtaagag gctctgaccg agatggtggg
     1381 gcagatggca tgtcagcgga atgtgagtgc aacattaaaa tcctcgatgt caatgataat
     1441 atcccttaca tggaacagtc ttcatatacc atagaaattc aagaaaatac tctaaattca
     1501 aatttgctcg aaattagagt aattgatttg gatgaagagt tctcagctaa ctggatggca
     1561 gtaattttct ttatctctgg aaatgaagga aattggtttg agatagaaat gaatgaaaga
     1621 acaaatgtgg gaattttaaa ggttgttaag cccttagatt atgaagctat gcagagtctg
     1681 caactcagta ttggtgtcag aaataaagct gaatttcatc attcaattat gtctcaatat
     1741 aaactgaaag catctgcaat ttctgtgact gtgttaaatg taattgaagg cccagtgttt
     1801 cgtccaggtt caaagacata tgttgtaact ggtaatatgg gatcaaatga taaagtggga
     1861 gactttgtag ctactgacct ggacacaggt agaccttcaa cgactgttag gtatgtaatg
     1921 ggaaataatc cagctgacct gcttgctgtt gattcaagaa caggcaaact cactttgaaa
     1981 aataaagtta ccaaggaaca gtacaatatg ctcggaggaa aataccaagg aacgattctc
     2041 tctatagatg ataatcttca aagaacttgc actggtacaa ttaatattaa cattcaaagt
     2101 tttggtaatg acgacaggac taatacagag ccgaacacta aaattactac caatactggc
     2161 agacaagaaa gtacttcttc cactaactat gataccagca caacttctac tgactctagc
     2221 caagtatatt cttctgaacc cggaaacgga gccaaagatt tgttatcaga caatgtacat
     2281 tttggtcctg ctggcattgg actcctcatc atgggattct tggtcttagg attggtccca
     2341 tttttgatga tctgttgtga ttgtggaggt gctcctcgta gtgcagctgg ctttgagcct
     2401 gttcccgaat gttcagatgg agcaattcat tcatgggcag tagaaggacc acagcctgaa
     2461 cccagggata taaccactgt cataccacaa ataccacctg ataacgcaaa tataattgaa
     2521 tgcattgaca actcaggagt ttatacaaat gagtatggtg gcagagaaat gcaagacctg
     2581 ggaggaggag agagaatgac aggatttgaa ctaacagagg gagttaaaac ttcaggaatg
     2641 cctgagatat gtcaagaata ctctggaaca ttaagaagaa attctatgag ggaatgtaga
     2701 gaaggaggtc tgaatatgaa tttcatggaa agctacttct gtcagaaagc atatgcttac
     2761 gcagatgaag atgaaggacg cccatctaat gactgtttgc tcatatatga catcgaaggt
     2821 gtaggttccc ctgctggctc tgtgggttgt tgtagcttca ttggagaaga cctggatgac
     2881 agcttcttgg ataccctggg acctaaattt aagaagttgg cagacatcag cctaggaaaa
     2941 gaatcatatc cagaccttga tccttcttgg ccaccacaaa gcactgaacc agtttgcctt
     3001 cctcaggaaa cagagcccgt tgttagtgga cacccaccaa tctccccaca tttcggcact
     3061 accacagtaa tttctgagag cacctatccc tcgggacctg gtgtactgca tcctaagcct
     3121 attctcgatc ctctgggcta tggtaatgtc actgtgaccg agtcttacac cacctctgac
     3181 actctgaagc cctctgtgca cgttcacgat aaccgaccag catcaaacgt ggtagtgaca
     3241 gagagagtgg tcggcccaat ctctggcgct gatttgcatg gaatgttaga gatgcctgac
     3301 ttgcgagatg ggtcgaatgt tatagtgaca gaaagggtaa tagcaccaag ctctagtcta
     3361 cccacctctc tgactatcca tcatcctaga gagtcttcaa atgtggtagt gacagaaaga
     3421 gtaatccaac caacttccgg catgataggt agtctgagta tgcaccccga gttagccaat
     3481 gcccacaatg tcattgtgac agagagggtt gtttctggtg ctggcgtaac tggaattagt
     3541 ggcaccactg ggatcagcgg tggcataggc agcagtggcc tggttggcac cagcatgggt
     3601 gctgggagcg gtgccctgag tggagctggc ataagtggtg gtggcattgg cctgagcagc
     3661 ttgggaggga cagccagcat tggccacatg aggagttcct ctgaccatca ctttaaccaa
     3721 accattgggt ccgcctcccc tagcacagct cgaagtcgaa tcacaaagta tagtaccgtg
     3781 caatatagca agagcagcgg tggagcaagc gcagccagtg gtagcgcgga tccaccggtc
     3841 gccaccatgg tgagcaaggg cgaggagaat aacatggcca tcatcaagga gttcatgcgc
     3901 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     3961 gagggccgcc cctacgaggc ctttcagacc gctaagctga aggtgaccaa gggtggcccc
     4021 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggtctacatt
     4081 aagcacccag ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaggtgg
     4141 gagcgcgtga tgaacttcga ggacggcggc attattcacg ttaaccagga ctcctccctg
     4201 caggacggcg tgttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     4261 cccgtaatgc agaagaagac catgggctgg gaggcctccg aggagcggat gtaccccgag
     4321 gacggcgccc tgaagagcga gatcaagaag aggctgaagc tgaaggacgg cggccactac
     4381 gccgccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     4441 atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg actacaccat cgtggaacag
     4501 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagcg
     4561 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4621 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     4681 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4741 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4801 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     4861 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     4921 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     4981 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     5041 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     5101 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     5161 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     5221 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     5281 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5341 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     5401 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5461 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5521 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5581 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5641 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5701 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     5761 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     5821 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     5881 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     5941 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     6001 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6061 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6121 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6181 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6241 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6301 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6361 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6421 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6481 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6541 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     6601 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     6661 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6721 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     6781 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     6841 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     6901 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     6961 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     7021 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7081 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7141 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7201 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7261 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7321 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7381 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7441 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7501 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7561 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7621 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7681 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7741 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7801 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt t
//
