LOCUS       sequence-444176-        7852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7852)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Desmoglein1-N-18
FEATURES             Location/Qualifiers
     source          1..7852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        102..405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        406..609
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     560..580
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      641..650
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3842..3851
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3848..4558
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3993..4011)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4261..4280
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4681..4802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4718..4737)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4772..4791
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4809..5264)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4946..4967)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5158..5177
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5291..5395
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5397..5754
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5397..5588
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5420..5440)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5605..5740
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5789..6583
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5789..6583
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5843..5862)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6453..6472
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6771..6790)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6815..6862
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7191..7779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7680..7699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
       61 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      121 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      181 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      241 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      301 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      361 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      421 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      481 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      541 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      601 agcagagctg gtttagtgaa ccgtcagatc cgctagcgtc gccaccatgg actggagttt
      661 cttcagagta gttgcagtgc tgttcatttt tctggtggtg gtagaagtta acagtgagtt
      721 ccgaatccag gtaagagatt ataacactaa aaatggcacc atcaaatggc attcaatccg
      781 aaggcagaaa cgtgaatgga tcaagttcgc agcagcctgt cgtgaaggtg aagacaactc
      841 aaagaggaac ccaatcgcca aaattcactc agattgtgct gcaaaccagc aagttacata
      901 ccgcatctct ggagtaggaa ttgatcagcc accatatggg atctttgtca ttaatcagaa
      961 aactggtgaa attaatataa catccatagt tgatcgagag gtcactcctt tcttcattat
     1021 ctactgccga gctctgaact caatgggcca agatttagag aggcctctag agctcagagt
     1081 cagggttttg gatataaatg acaaccctcc agtgttttca atggctacat ttgcaggaca
     1141 aatagaagaa aattctaatg caaatacact ggtgatgata ctcaatgcta ctgacgcaga
     1201 tgaaccgaac aatttgaact caaaaatagc cttcaagatt ataagacaag aaccttcaga
     1261 ttcaccaatg tttattatca acagaaatac tggagaaatt cgaacgatga ataattttct
     1321 agacagagag caatacggcc agtatgctct tgctgtaaga ggctctgacc gagatggtgg
     1381 ggcagatggc atgtcagcgg aatgtgagtg caacattaaa atcctcgatg tcaatgataa
     1441 tatcccttac atggaacagt cttcatatac catagaaatt caagaaaata ctctaaattc
     1501 aaatttgctc gaaattagag taattgattt ggatgaagag ttctcagcta actggatggc
     1561 agtaattttc tttatctctg gaaatgaagg aaattggttt gagatagaaa tgaatgaaag
     1621 aacaaatgtg ggaattttaa aggttgttaa gcccttagat tatgaagcta tgcagagtct
     1681 gcaactcagt attggtgtca gaaataaagc tgaatttcat cattcaatta tgtctcaata
     1741 taaactgaaa gcatctgcaa tttctgtgac tgtgttaaat gtaattgaag gcccagtgtt
     1801 tcgtccaggt tcaaagacat atgttgtaac tggtaatatg ggatcaaatg ataaagtggg
     1861 agactttgta gctactgacc tggacacagg tagaccttca acgactgtta ggtatgtaat
     1921 gggaaataat ccagctgacc tgcttgctgt tgattcaaga acaggcaaac tcactttgaa
     1981 aaataaagtt accaaggaac agtacaatat gctcggagga aaataccaag gaacgattct
     2041 ctctatagat gataatcttc aaagaacttg cactggtaca attaatatta acattcaaag
     2101 ttttggtaat gacgacagga ctaatacaga gccgaacact aaaattacta ccaatactgg
     2161 cagacaagaa agtacttctt ccactaacta tgataccagc acaacttcta ctgactctag
     2221 ccaagtatat tcttctgaac ccggaaacgg agccaaagat ttgttatcag acaatgtaca
     2281 ttttggtcct gctggcattg gactcctcat catgggattc ttggtcttag gattggtccc
     2341 atttttgatg atctgttgtg attgtggagg tgctcctcgt agtgcagctg gctttgagcc
     2401 tgttcccgaa tgttcagatg gagcaattca ttcatgggca gtagaaggac cacagcctga
     2461 acccagggat ataaccactg tcataccaca aataccacct gataacgcaa atataattga
     2521 atgcattgac aactcaggag tttatacaaa tgagtatggt ggcagagaaa tgcaagacct
     2581 gggaggagga gagagaatga caggatttga actaacagag ggagttaaaa cttcaggaat
     2641 gcctgagata tgtcaagaat actctggaac attaagaaga aattctatga gggaatgtag
     2701 agaaggaggt ctgaatatga atttcatgga aagctacttc tgtcagaaag catatgctta
     2761 cgcagatgaa gatgaaggac gcccatctaa tgactgtttg ctcatatatg acatcgaagg
     2821 tgtaggttcc cctgctggct ctgtgggttg ttgtagcttc attggagaag acctggatga
     2881 cagcttcttg gataccctgg gacctaaatt taagaagttg gcagacatca gcctaggaaa
     2941 agaatcatat ccagaccttg atccttcttg gccaccacaa agcactgaac cagtttgcct
     3001 tcctcaggaa acagagcccg ttgttagtgg acacccacca atctccccac atttcggcac
     3061 taccacagta atttctgaga gcacctatcc ctcgggacct ggtgtactgc atcctaagcc
     3121 tattctcgat cctctgggct atggtaatgt cactgtgacc gagtcttaca ccacctctga
     3181 cactctgaag ccctctgtgc acgttcacga taaccgacca gcatcaaacg tggtagtgac
     3241 agagagagtg gtcggcccaa tctctggcgc tgatttgcat ggaatgttag agatgcctga
     3301 cttgcgagat gggtcgaatg ttatagtgac agaaagggta atagcaccaa gctctagtct
     3361 acccacctct ctgactatcc atcatcctag agagtcttca aatgtggtag tgacagaaag
     3421 agtaatccaa ccaacttccg gcatgatagg tagtctgagt atgcaccccg agttagccaa
     3481 tgcccacaat gtcattgtga cagagagggt tgtttctggt gctggcgtaa ctggaattag
     3541 tggcaccact gggatcagcg gtggcatagg cagcagtggc ctggttggca ccagcatggg
     3601 tgctgggagc ggtgccctga gtggagctgg cataagtggt ggtggcattg gcctgagcag
     3661 cttgggaggg acagccagca ttggccacat gaggagttcc tctgaccatc actttaacca
     3721 aaccattggg tccgcctccc ctagcacagc tcgaagtcga atcacaaagt atagtaccgt
     3781 gcaatatagc aagagcagcg gtggagcaag cgcagccagt ggtagcgcgg atccaccggt
     3841 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     3901 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     3961 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     4021 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     4081 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     4141 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     4201 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     4261 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     4321 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     4381 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     4441 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     4501 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaag
     4561 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     4621 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     4681 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4741 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4801 taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     4861 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4921 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4981 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     5041 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     5101 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     5161 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     5221 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     5281 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5341 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     5401 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5461 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5521 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5581 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5641 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5701 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     5761 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5821 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5881 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5941 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     6001 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6061 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6121 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6181 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6241 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6301 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6361 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6421 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6481 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6541 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6601 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6661 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6721 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     6781 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     6841 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     6901 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     6961 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     7021 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     7081 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7141 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7201 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7261 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7321 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7381 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7441 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7501 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7561 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7621 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7681 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7741 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7801 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gt
//
