LOCUS       sequence-444193-        6734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SLC17A2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6734)
  TITLE     SLC17 transporters mediate renal excretion of Lac-Phe in mice and 
            humans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDNA3.SLC17A2-flag
FEATURES             Location/Qualifiers
     source          1..6734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             521..544
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(584..602)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(585..602)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(622..639)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    628..852
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      898..1326
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(985..1004)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1195..1216
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1335..1355)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1340..1669
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1520..1655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1582..1601
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1736..2530
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1790..1809)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2400..2419
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2704..2825
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2741..2760)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2795..2814
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2874..2890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2874..2890)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2887..2909)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2898..2914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2922..2928,2929..2946,2947..2952))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2967..2988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3105..3122)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3276..3864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3356..3375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4035..4826,4827..4895))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4658..4677
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4896..5000)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5075..5094)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5266..5645
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5646..5849
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5800..5820
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5894..5913
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5894..5912
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gatggtcaca tgcctaccac tttgggccat tttcctgggt tttttcagcc atttctggtt
       61 gtgcaccatc atcctaacat acctaccaac gtatatcagt actctgctcc atgttaacat
      121 cagagatagt ggagttctgt cctccctgcc ttttattgct gctgcaagct gtacaatttt
      181 aggaggtcag ctggcagatt tccttttgtc caggaatctt ctcagattga tcactgtgcg
      241 aaagctcttt tcatctcttg atatgcaagt ttcctcatgg gaatctcaag gggatttggg
      301 ctcatcgcag gaatcatctc ttccactgcc actggattcc tcatcagtca ggattttgag
      361 tctggttgga ggaatgtctt tttcctgtct gctgcagtca acatgtttgg cctggtcttt
      421 tacctcacgt ttggacaagc agaacttcaa gactgggcca aagagaggac ccttacccgc
      481 ctctgaggac ataaagttac aaactggcgg atctggcagt gattacaagg atgacgacga
      541 taaggtttaa tgatggctcg agcatgcatc tagagggccc tattctatag tgtcacctaa
      601 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
      661 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
      721 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
      781 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
      841 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
      901 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      961 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     1021 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     1081 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     1141 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     1201 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     1261 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     1321 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     1381 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     1441 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     1501 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     1561 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     1621 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     1681 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     1741 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     1801 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     1861 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     1921 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     1981 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     2041 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     2101 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     2161 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     2221 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     2281 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     2341 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     2401 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     2461 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     2521 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     2581 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     2641 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     2701 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2761 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2821 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     2881 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2941 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     3001 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     3061 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacaaaccc
     3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4321 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     5041 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5101 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     5161 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     5221 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     5281 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     5341 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     5401 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     5461 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     5521 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     5581 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     5641 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     5701 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     5761 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     5821 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
     5881 ggcttatcga aattaatacg actcactata gggagaccca agcttatcta gatggacggg
     5941 aagcctgcca ccaggaaagg tccagatttc tgttcattac gctatgggct ggctcttatc
     6001 atgcacttct caaacttcac catgataacg cagcgtgtga gtctgagcat tgcgatcatc
     6061 gccatggtga acaccactca gcagcaaggt ctatctaatg cctccactga ggggcctgtt
     6121 gcagatgcct tcaataactc cagcatatcc atcaaggaat ttgatacaaa ggcctctgtg
     6181 tatcaatgga gcccagaaac tcagggtatc atctttagct ccatcaacta tgggataata
     6241 ctgactctga tcccaagtgg atatttagca gggatatttg gagcaaaaaa aatgcttggt
     6301 gctggtttgc tgatctcttc ccttctcacc ctctttacac cactggctgc tgacttcgga
     6361 gtgattttgg tcatcatggt tcggacagtc cagggcatgg cccagggaat ggcatggaca
     6421 ggtcagttta ctatttgggc aaagtgggct cctccacttg aacgaagcaa gctcaccacc
     6481 attgcaggat cagggtcagc atttggatcc ttcatcatcc tctgtgtggg gggactaatc
     6541 tcacaggcct tgagctggcc ttttatcttc tacatctttg gtagcactgg ctgtgtctgc
     6601 tgtctcctat ggttcacagt gatttatgat gaccccatgc atcacccgtg cataagtgtt
     6661 agggaaaagg agcacatcct gtcctcactg gctcaacagc ccagttctcc tggacgagct
     6721 gtccccataa aggc
//
