LOCUS       sequence-444194-       14693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CDK4 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14693)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 14693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:CDK4_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..14693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5514..5523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6441..6575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6596..6730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7032..7745,7746..7748,7749..7751))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7035..7745,7746..7748,7749..7751))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7077..7098)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7426..7445
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7685..7706
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8932..8948)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8932..8948)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9003..9021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9031..9048)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9031..9047)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9040..9062)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9188..9643)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9325..9346)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9537..9556
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9670..9774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9775..9843,9844..10635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9993..10012)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10806..11394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11295..11314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11548..11565
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11682..11703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11718..11723,11724..11741,11742..11748)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11756..11772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11761..11783
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11780..11796
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11780..11796
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11815..11835
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11817..11835
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg gtcctaatgc cccagtataa
       61 accagcaggt caacctgtgg tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc
      121 wacctgtggt ctgtgtgggt cctaatgccc cagtataaac caacaggtca acctgtggtc
      181 tgtgtgggtc ctaatgcccc agtataaacc agcagrtcaa cctgtggtct gtgtgggtcc
      241 taatgcccca gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct aatgccccrg
      301 tataaaccag caggtcaacc tgtggtctgt gtgggtccta atgccccagt ataaaccagc
      361 aggtcaacct gtggtctgtg tgggtcctaa tgccccagta taaaccagca ggtctacctg
      421 tggtctgtgt gggtcctaat gcaccagtat aaaccagcag gtcaacctgt ggtctgtgtg
      481 ggttctaatg ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg gtccaaatgc
      541 cccagtacaa accagcaggt caacctgtgg tctgtgtggg tcctaatgcc ccagtataaa
      601 ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc cggtacaaac cagcatgtca
      661 acctgtggtc tgtgtgggtc ctaatgcccc ggtataaacc agcagrtcaa cctgtggtct
      721 gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct
      781 aatgccccag tataaaccag cakgtcaacc tgtggtctgt gtgggtccta atgccccagt
      841 ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa tgccccagta taaaccagca
      901 ggtcaacctg tggtctgtgt gggtcctaat gccccrgtat aaaccagcak gtcaacctgt
      961 ggtctgtgtg ggtcctaatg ccccnggtat aaaccagcag atcaacctgt ggtctgtgtg
     1021 ggtcctaatg ccccagtata aaccagcaga tcaacctgtg gtctgtgtgg gtcctaaang
     1081 tcccaggata aaccagcatg tcaacctgtg gtctgtgtgg gtcctaatgc cccggtataa
     1141 accagcagat caacctgtgg tctgtgtggg tcctaatgcc ccaggataaa ccagcaggtc
     1201 aacctgtggt ctgtgtgggt cctaatgccc cagtataaac cagcaggtca acctgtggtc
     1261 tgtgtgggtc ctaatgcccc agtatattga tggagactct atactgctcg gtgagctcca
     1321 tctttcggat aagatgttaa aacaaggtcc cgactctctg tggtggtaaa aaattcccag
     1381 gatgtccttc ctaccctggc atcctggaca aatttgctca ctgtcctctg tccatcatgg
     1441 cctcctaacc atccccatat cataattggc tttatcactc tagctggggc cgaaatcgca
     1501 tacttccatt ttatgaggta cgctaaaatc agtatgcgag ccaagtaata tgtccgaata
     1561 cttccggcga gattctagag tgtgcattcg atgcacgcta cgctattcca tgatgccccg
     1621 tgagagaatt cttgaatcgg agagaagcaa cgcaactcat gcgggtaggt tacgtgacga
     1681 tgacatattg gcggatgtag tacgtccaag ttccattcat actgctcaca ttcatatttt
     1741 atagaatgtt cttttcccac agccaagtag taagtttaaa ttcaaataca gtacctacta
     1801 agtagtagta gacggtttcg gacgcagccc ctgtctcctc tccaccaata tactggtgtg
     1861 tagtgtgcgg tctggtgcaa tatggctgcc atcgcgtcat ccaggtggat gctgcacact
     1921 ggtggttgat gaggagattt tgcccaaaaa agtgttttga gtttccagaa aagcgcgata
     1981 taaatgtaag gaattatcat tagcttatta taatggaact ttctggataa cttgtgccag
     2041 agctaggggc cattaaaact ttgccctggg gtacctcagg gagctgcaag gaagtgctat
     2101 agggctaatg aaatgtttgg gggaaaaaaa aaaattttat aagtaaaaat aaaactaaaa
     2161 taattaataa tttcattgtt gaaaattata gtaaaattga taaacaagat aagcagcatt
     2221 agtataaaaa actaaaaaca aacgtaattt aaaataatta aattgtttta aataaattat
     2281 acaaaataat gtttacaacg acacggtggt gcagtaggta gtgctgtcgc cagcaaagca
     2341 agaaggtcgc tggttcgagc ctcagctgga tcacttgatg tttctgtgtg gagcattcgc
     2401 gtgcgtttaa ccccagatta ataaagagac taagccgaaa agaaaataaa tgtatgaaaa
     2461 tgtttacgta aaatgtaaat aaatgcatga ttaataaaat atatgaattt taaagattga
     2521 ggtagaattg ttttattatt cacacatttg gttattttta atcagtgaca acttgtgatg
     2581 ctctttatat tgtttattac gctactttga atattcagtc tcaaattgca tgtaaatacg
     2641 acttcaaagc agaattagtt taatttaatt gcctgtaaac agtgttgtac tttgatggtg
     2701 attgtctgat aataatatga tttcactatt taaaatttga aaaaaaaaaa tctgaaaaat
     2761 actcaccggc cactttatta ggtacacctg tctaactgct tgttaacgta aatttctaat
     2821 cagccaatca ccaggcagca actcagtgca tttaggcatg tagacatggt caagacgatc
     2881 tgctgcagtt caaagcgagc atcagaatgg agaagcaagg tgatttaagt gactttaaac
     2941 gtgacgtgtt tgttggtgcc agacgggctg gtctgagtat ttcagaaact gctgatttac
     3001 tggggttttc acacacaaac atctctgggg tttacagaga atggtccaaa aaaagataat
     3061 atccagtgag cggcagttct gtgggggcaa atgtcttgtt gatgccagag gtcagaggag
     3121 aatggcctga ctggttccag ctgatagaaa ggcaacagta attcaaataa ccactcgtta
     3181 gaactgaggc atgcagaaga gcacctttga acacaacatg tcgaaccttg aggcgggtgg
     3241 gctacagcag ctcaagacca catcgggtgc cactcctgtc agctaagaac aggaaactga
     3301 ggctacaatt cgcacaggct caacataagt agacaataga agattgaaca ttatgttgcc
     3361 tggtcagatg gatacattct accttgtatc aacagttcag gctggtgatg gtggtgtaat
     3421 ggtgtggggg atatattctt ggcacacttt gggcccatta gtaccaatta accatcgtgt
     3481 caacgccaca gcctaactga gtattgttgc tgaccatgtt catttctttt tgaccacagt
     3541 gtacccatct tctgatggct acttcctgca ggataatgca ccatgtcata aagcgtgaat
     3601 gatctcagac tggtttcttg aacatgacaa tgagttcact gtgctcgaat gacctccaca
     3661 gtcaccagat ctcaatccaa tagagcgcct ttggggtgtg gtagaatgga agattcgcat
     3721 cctggatgtg cagccgacaa atctgcatga tgctagcatg tcaatatgga ccaaaatctc
     3781 tgaggaattt tccagtacct ggttgaaatg caaaaggaag ggaatctatg ctaaggcaaa
     3841 aagaggtcca acccagtact agtggggtgt acataataaa gtggccggtg agtgtatatt
     3901 gttaaggata tctagataat ccaaataatt tacagcacta tttaaaaaaa aaactttatg
     3961 tcaccaagtc tgcatttatt tgatcacaat tacagtcgaa aaagcaatat cgggatacat
     4021 tgtttccatt taaaataact atattatatt ttaaatattt ttgacaatgt tatttcttta
     4081 atgccaaagc taaattgtat tcaatctgaa ttttagtgtc acatgatcct tcagaaaata
     4141 ttaacacatt gctaaaaatg tatattatta tcaatttgga tttttttttt ttttttgtat
     4201 attaacctat gctattggtt actatacaaa agtattactg tgaatatatg catatacaga
     4261 accgtatcaa gcgcgggggg tggttgggtt atgtccccat ttggaaagac ctggaaacat
     4321 tcataatgac taaaattgca cggaaaaata taatatctat gagtattgct gatcagcagc
     4381 acaaaaccag aactccatat taataaacag ccaatgatgc agtgagtttg tgcatgtttt
     4441 cattcaaact cgtgtgattg tgctcaaaca cctgttgtga gcagcagttg caaacagtaa
     4501 acctcaagag gagccagtca accttcacaa acactcaacc tctggctcac tcactaaaca
     4561 attcgcgtta atatctaaaa taaaaatttc cagcaccaaa cagctctcag atttcggagt
     4621 acactgacca agaggtaaga agtaacgtta cattactagg acgactattg taagactaga
     4681 tatgtaatat ttggaagcgt acgtgtttta agtgacatat agagtagagg aactgatgga
     4741 aatacgattt gcataatgta aaatgatata tattaaaata taggctacag taggtaacgt
     4801 ggtttacata taacgttact gtgttaatgt agtgctcagt tactcgtgtt gacagttatt
     4861 attagtgctt gctaatttgt ttgttaatgc ttctcttgcg tatttgatta ccttcaggcg
     4921 aatctaaaac atatcagatg tagaaacggc agaaaaatgt tgaagaaaat acgagaatag
     4981 tagcttttgg agcacataga aaagtacatc agataaagcc taccgtttaa acgcagacgc
     5041 cgctctcgca ctgaatgctg ggtactgtag tccttatatt gtatgcttaa tgtgcgtctc
     5101 aacgggaaac tagagttagg aaaactacaa tttcctgtgg aaaatgtttc tgttttcggg
     5161 gaaacagcga aagaaagata tggttccgcg tttttttctt actttgaata ataaaataag
     5221 acctaactac acacttcgct tactcttatg ctaatttgaa gcactagcat tctttgatac
     5281 aatgataatc atttaaacat cacagttata ctacaaatac tatggttaaa ctatgtctag
     5341 tgtagtgtaa cactatggtt gccattgtta tctttaagtt taagtattat ctgtggtaaa
     5401 gccatggttg gtttttataa gaggtaataa aattaacatt gtacaattaa atgactataa
     5461 attaatgttt ttttttattt tttttacaca gctaccttca aaaagggatc cccgccacca
     5521 tggctacctc tcgatatgag ccagtggctg aaattggtgt cggtgcctat gggacagtgt
     5581 acaaggcccg tgatccccac agtggccact ttgtggccct caagagtgtg agagtcccca
     5641 atggaggagg aggtggagga ggccttccca tcagcacagt tcgtgaggtg gctttactga
     5701 ggcgactgga ggcttttgag catcccaatg ttgtccggct gatggacgtc tgtgccacat
     5761 cccgaactga ccgggagatc aaggtaaccc tggtgtttga gcatgtagac caggacctaa
     5821 ggacatatct ggacaaggca cccccaccag gcttgccagc cgaaacgatc aaggatctga
     5881 tgcgccagtt tctaagaggc ctagatttcc ttcatgccaa ttgcatcgtt caccgagatc
     5941 tgaagccaga gaacattctg gtgacaagtg gtggaacagt caagctggct gactttggcc
     6001 tggccagaat ctacagctac cagatggcac ttacacccgt ggttgttaca ctctggtacc
     6061 gagctcccga agttcttctg cagtccacat atgcaacacc tgtggacatg tggagtgttg
     6121 gctgtatctt tgcagagatg tttcgtcgaa agcctctctt ctgtggaaac tctgaagccg
     6181 accagttggg caaaatcttt gacctgattg ggctgcctcc agaggatgac tggcctcgag
     6241 atgtatccct gccccgtgga gcctttcccc ccagagggcc ccgcccagtg cagtcggtgg
     6301 tacctgagat ggaggagtcg ggagcacagc tgctgctgga aatgctgact tttaacccac
     6361 acaagcgaat ctctgccttt cgagctctgc agcactctta tctacataag gatgaaggta
     6421 atccggagtg aatcgatgat gatccagaca tgataagata cattgatgag tttggacaaa
     6481 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     6541 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattcg atgatgatcc
     6601 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6661 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6721 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     6781 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     6841 agatcagatc tcttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6901 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6961 aatgtatctt atcatgtctg gatctacgta atacgactca ctatagttct agaggctcga
     7021 gaggggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     7081 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
     7141 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     7201 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
     7261 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
     7321 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
     7381 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
     7441 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
     7501 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
     7561 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
     7621 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
     7681 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcttcgccc
     7741 ttgctcacca tggtcactgt ctgctttgct gttggtctgg gctcctgggt cactggctta
     7801 ctaatggagt ctttatgtat gaggactctt atcaattgtt cttctataaa ggtctgcagt
     7861 gtttctgttc gtcccctaca tggacaccca gagcctccta aatacaggag ccctgataac
     7921 tgcacaagtg ctcagattcc agcagggtgg aaatgagata aagtgtgcag atggggaggg
     7981 ggacgtgaat gagagatttg agggatgaaa aggatggatg aacgcattga aaatagcccc
     8041 tttcacaaaa taataccagt aaattgtcat ataattaatg accagacctt tacctgtaaa
     8101 tgatgtgatg ttcatataca gaagaatgcc aacaatttac agatgatttt acaaattctt
     8161 aattttcctt ttctaaatgg atttgttcac acattatctc ttcaaagcaa ttgaattttc
     8221 tggaaaagat tggctgtgtg tgtaaaaggt gctactgagg aatttaatgt gacatggaat
     8281 gaagcaaaca gcagcctaag acaggatggc aggaaaaatg tcacatgttt aaaataagag
     8341 tgaaaccaac gacctggact tatagagact gtgtgtcctt aggcaaagct cttgtacaaa
     8401 cagtgcttgt gactgtgaaa ttcggggttt gcctggattg tgttaaatgt gtgcttggat
     8461 gtatcataaa aaagaatcgg ttctctgtat ttgacatttt ctgtaattta tctaaagtta
     8521 caactggcta tgcctgattt ttatcactat tgaatcaatc attttaaaga atgttaagtc
     8581 tgctgatttg gagagctcat ttacatttat tttgaatgtc tcttatttaa cacaactgat
     8641 ttaagcttta gatctgcgaa gatacggcca cgggtgctct tgatcctgtg gctgattttg
     8701 gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct tccactgatt
     8761 gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt aatagatgtg
     8821 acatgattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     8881 caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagatc agatccacta
     8941 gttctagagc ggccattacc ctgttatccc taggccgcca ccgcggtgga gctccaattc
     9001 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     9061 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     9121 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     9181 cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     9241 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     9301 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     9361 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     9421 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     9481 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     9541 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     9601 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     9661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9721 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9961 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
    10021 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    10081 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    10141 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10201 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10261 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    10321 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    10381 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    10441 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    10501 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    10561 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    10621 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10681 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10921 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    11041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    11101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    11161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    11221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    11281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    11341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    11401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    11461 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    11521 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    11581 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    11641 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    11701 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    11761 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
    11821 aaccctcact aaagggaaca aaagctgggt accgggcccc ccctcgaatt accctgttat
    11881 ccctatcgag caaaatcaat cctaatgtaa aatagtatac gttcatcatt ataatgcaaa
    11941 cactgtagaa ctgttgtgtt gtgccttgca tttcattctc agtttcttat cgtctgtctc
    12001 tttttacgac agagctagaa aaaactagcc ttcatttgcc atcgccgtat gcaaatccac
    12061 tccagaactc aacaggtttg ttctgcggcg ggtgtaagtg tgaacaccgg catgtaagtg
    12121 cgcttgagca ggagaataaa gcgctcagag acaccggagg aatcctttat tttgcgttgc
    12181 gcctcagggg attcgctgag ccttctcttc atggccctgc tcttcagaca caggtgctcc
    12241 gctgtttgtg tttttcactg ttcttcaggc actcgtggtt acgggttttt gttttgcact
    12301 cttgcacaca catctctcgg cctccaaaac aaacacacaa ctgcacgagg gctcgaagcc
    12361 cccaccgaaa aacaaaaaca ttatgctaat gaggaaggca cagctgttcc cttcttcact
    12421 ctctctctcg ttctttcaac aacagtcgcc taatttctct cgttccttta aagagccgcc
    12481 aagcagaaac agttaatgca aatataagcc tgcgggaaat aaggagaatt tttacaaaat
    12541 ccgagcttcc cctcctgcca aaatgtgatg tgtgactcac gcattcgata tttggtgtaa
    12601 tgagagtgaa tcattctgct ccacaccgaa cccgatgcca cagaccactc agatgcacac
    12661 cgaggagact attttaacag cacattacat ccagactgtc acagatgcat taccaagagt
    12721 gtgatttcag tgtcgaatgc aggtcttaaa ggaaaatctg ttgcgattgc atgtgcgcta
    12781 actagacacg accaacgaat gggaacaatc tgtgaaatat tatacaatca caggcaatta
    12841 aaagatgttt aaagttcaat ttagttttaa ttaatatgtt atataaaact aatctgtttt
    12901 aaatgtatat ttatgatttt agctttagct agcctcttga ccagcagctg aagtgtccgg
    12961 caagtaccta aaagggagac cacatgggaa agctaggttg ctcccggaag aggtgttagt
    13021 aaaccagcag gtctacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg
    13081 tcaacctgtg gtctgtgtgg gtcctaatgc cccagtataa accagcaggt ctacctgtgg
    13141 tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc aacctgtnag tctgtgtggg
    13201 tcctaatgcc ccagtataaa ccagcaggtc tacctgtggt ctgtgtgggt cctaatgccc
    13261 cagtataaac cagcaggtca acctgtagtc tgtgtgggtc ctaatgcccc agtataaacc
    13321 agcaggtcta cctgtggtct gtgtgggtcc taatgcccca gtataaacca acaggtcaac
    13381 ctgtgatctg tgtgggtcct aatgccccag tataaaccag caggtcaacc tgtggtctgt
    13441 gtgggtccta atgccccagt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa
    13501 tgccccagta taaaccagca ggtcaacgtg tggtctgtgt gggtcctaat gccctggtat
    13561 aaaccagcag gtcaacttgt ggtctgtgtg ggtcctaatg ccccggtata aaccagcatg
    13621 tcaacctgtg gtctgtgtgg gtcctaatgc cccggtataa accagcagrt caacctgtgg
    13681 tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt
    13741 cctaatgccc cggtayaaac cagcatgtca acctgtggtc tgtgtgggtc ctaatgcccc
    13801 agtataaacc agcaggtcaa cctgtggtct gtgtgggtcc taatgcccca gtataaacca
    13861 gcaggtctac ctgtggtctg tgtgggtcct aatgccccag tataaaccag caggtcaacc
    13921 tgtggtctgt gtgggtccta atgccccagt ataaaccagc aggtctacct gtggtctgtg
    13981 tgggtcctaa tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtcctaat
    14041 gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata
    14101 aaccagcagg tcaacctgtg gtctgtgtgg gtcctaatgc cccagtataa accagcaggt
    14161 caacctgtgg tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt
    14221 ctgtgtgggt cctaatgccc cagtatattg atggagactc tatactgctc ggtgagctcc
    14281 atctttcgga taagatgtta aaacaaggtc ccgactctct gtggtggtaa aaaattccca
    14341 ggatgtcctt cctaccctgg catcctggac aaatttgctc actgtcctct gtccatcatg
    14401 gcctcctaac catccccata tcataattgg ctttatcact ctagctgggg ccgaaatcgc
    14461 atacttccat tttatgaggt acgctaaaat cagtatgcga gccaagtaat atgtccgaat
    14521 acttccggcg agattctaga gtgtgcattc gatgcacgct acgctattcc atgatgcccc
    14581 gtgagagaat tcttgaatcg gagagaagca acgcaactca tgcgggtagg ttacgtgacg
    14641 atgacatatt ggcggatgta gtacgtccaa gttccattca tactgctcac att
//
