LOCUS       sequence-444198-       12696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Csy4 with 5' and 3' targets (I) and without Cas for Lotus callus 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Bircheneder M, Schreiber T, Tissier A, Parniske M
  TITLE     A quantitative assay for the efficiency of RNA-guided genome editing
            in plants.
  JOURNAL   Plant J. 2024 Sep;119(5):2564-2577. doi: 10.1111/tpj.16931. Epub 
            2024 Jul 20.
  PUBMED    39032106
REFERENCE   2  (bases 1 to 12696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16931";
            journalName: "Plant J"; date: "2024-09"; volume: "119"; issue: "5"; 
            pages: "2564-2577"
COMMENT     Sequence Label: pMB20
FEATURES             Location/Qualifiers
     source          1..12696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        46..225
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        78..261
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             282..1307
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             2803..2820
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    2831..2850
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     CDS             join(2890..2892,2893..2895,2896..3606)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2935..2956)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3196..3215)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3543..3564
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3619..3639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3668..3920
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      6017..6265
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6329..6353
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7654..8283
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8712..9785
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9851..10045
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10281..10303)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10389..10529
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10444..10463
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10544..10561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10715..11303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10795..10814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(11373..11392)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11374..11392)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11397..11413)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11397..11413)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11515..11534)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             11526..12335
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(11593..11612)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    12591..12615
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga
       61 aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc
      121 attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa
      181 aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac
      241 tctcaatcca aataatctgc accggatctt ctggaagcac catgaaaaag cctgaactca
      301 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtgtcc gacctgatgc
      361 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
      421 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
      481 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggagttt agcgagagcc
      541 tgacctattg catctcccgc cgttcacagg gtgtcacgtt gcaagacctg cctgaaaccg
      601 aactgcccgc tgttctacaa ccggtcgcgg aggctatgga tgcgatcgct gcggccgatc
      661 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
      721 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
      781 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
      841 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
      901 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
      961 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     1021 gctacttcga gcggaggcat ccggagcttg caggatcgcc acgactccgg gcgtatatgc
     1081 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     1141 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     1201 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     1261 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa gaaatagaag ggctaagacc
     1321 taagctagcc tagaatccgg ccatgctaga gtccgcaaaa atcaccagtc tctctctaca
     1381 aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat
     1441 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
     1501 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga
     1561 ccttgagtga cctaagctag cctagtgtcg ggctagataa ttaggctaaa ctatccttaa
     1621 ggattgtaga taattaggct aaactatcct taaggtgtcg cggggagaga ggattttgag
     1681 gaaataatta attgaattac ttgtattatt gataaagtaa tttagataag ttgttagtac
     1741 aacttattgc aactatggat agacaaaaat cacttatttt aaggggagaa gtatataaca
     1801 acttattata aaatttctga gcagccggcc tcctccaatc aatcatagag agtggagccc
     1861 attctggaaa accaaggaac ccccaacttg cactggtgcg gtggcccaat tcaaaaaaaa
     1921 cggggcccag caaagtaacg ccgttgtaac gatttatcaa tccaaactca aaaggcggcg
     1981 agatgcgttt aactccgtga aattaacaaa ccgccaacaa cttgcaattt gcaactaccg
     2041 tttccaagaa gaactcaacc acacaacgta tcctatccca aaccacacgc aactagtgac
     2101 gcgtcataag gacacgtgtc acaatttgac tggttaataa tttcaccgct tttgctataa
     2161 attacctcca atccccttag cttcttcaca attcagttcc caaccctaac aattcttgtt
     2221 catatcgctt ctctctactt tcaaggtatg atccaatttc tctcttcttt ctctgtaatc
     2281 ctttcgttga gttttgtttc cgatcaatca taggtagttt tcttgtttcg aagcatgaga
     2341 tctaggaatt ttttgtgatt ttccaaaatt gagatcggtt tgaaattgaa ttttacagct
     2401 tgaatctcag atcttgtttt atcaatgttt tcgatggctt gcgatgtaga tctatgataa
     2461 ttgtggttca gttttgttag gaatcgattt cggtttagca attgcagatt aattagggtt
     2521 tccaattgaa ttcttcagat ccgttatgga attatgtcaa ataatttatt caaattggaa
     2581 attattgtta gatccactct taatctgttt gatccaagct tcaattaggg ttttcacttg
     2641 tttcaatttc ttgttatgga ttctgattta tctgttgatg ttagatccac tcttaatctg
     2701 tttgatccaa gcgttaatta gggttttcac ttgtttcaat ttcgtgtgtt ggattctgat
     2761 ttatctgttg ttgatgtgat tacagtctga acaatgggca gccaccacca ccaccaccac
     2821 agcggaatga gttcactgcc gtataggcag ctaagaaaat caggtggcac cggcggcagc
     2881 ggcggatcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2941 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     3001 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     3061 tggcccaccc tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac
     3121 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     3181 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3241 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     3301 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3361 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3421 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3481 gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     3541 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3601 tacaagaagg gactgcagcc taagaagaag agaaaggttg gaggagtcga ctcgagtgcg
     3661 taaaatcgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     3721 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     3781 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     3841 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     3901 gtcatctatg ttactagatc tgagtgacct aagctagcct agtgtctgag gcggccaaca
     3961 aactattcgt cacgtcgaca aaatttagaa cgaacttaat tatgatctca aatacattga
     4021 tacatatctc atctagatct aggttatcat tatgtaagaa agttttgacg aatatggcac
     4081 gacaaaatgg ctagactcga tgtaattggt atctcaactc aacattatac ttataccaaa
     4141 cattagttag acaaaattta aacaactatt ttttatgtat gcaagagtca gcatatgtat
     4201 aattgattca gaatcgtttt gacgagttcg gatgtagtag tagccattat ttaatgtaca
     4261 tactaatcgt gaatagtgaa tatgatgaaa cattgtatct tattgtataa atatccataa
     4321 acacatcatg aaagacactt tctttcacgg tctgaattaa ttatgataca attctaatag
     4381 aaaacgaatt aaattacgtt gaattgtatg aaatctaatt gaacaagcca accacgacga
     4441 cgactaacgt tgcctggatt gactcggttt aagttaacca ctaaaaaaac ggagctgtca
     4501 tgtaacacgc ggatcgagca ggtcacagtc atgaagccat caaagcaaaa gaactaatcc
     4561 aagggctgag atgattaatt agtttaaaaa ttagttaaca cgagggaaaa ggctgtctga
     4621 cagccaggtc acgttatctt tacctgtggt cgaaatgatt cgtgtctgtc gattttaatt
     4681 atttttttga aaggccgaaa ataaagttgt aagagataaa cccgcctata taaattcata
     4741 tattttcctc tccgctttga attgtctcgt tgtcctcctc actttcatca gccgttttga
     4801 atctccggcg acttgacaga gaagaacaag gaagaagtct aagagagaaa gtaagagata
     4861 atccaggaga ttcattctcc gttttgaatc ttcctcaatc tcatcttctt ccgctctttc
     4921 tttccaaggt aataggaact ttctggatct actttatttg ctggatctcg atcttgtttt
     4981 ctcaatttcc ttgagatctg gaattcgttt aatttggatc tgtgaacctc cactaaatct
     5041 tttggtttta ctagaatcga tctaagttga ccgatcagtt agctcgatta tagctaccag
     5101 aatttggctt gaccttgatg gagagatcca tgttcatgtt acctgggaaa tgatttgtat
     5161 atgtgaattg aaatctgaac tgttgaagtt agattgaatc tgaacactgt caatgttaga
     5221 ttgaatctga acactgttta aggttagatg aagtttgtgt atagattctt cgaaacttta
     5281 ggatttgtag tgtcgtacgt tgaacagaaa gctatttctg attcaatcag ggtttatttg
     5341 actgtattga actctttttg tgtgtttgca gctcataaaa atctgcccac gactcacata
     5401 gtccccctca aacaccatgg atcattatct tgatattaga cttagacctg atcctgaatt
     5461 tcctcctgct caacttatgt ctgttctttt tggaaagctt catcaagctc ttgttgctca
     5521 aggaggagat agaattggag tttcttttcc tgatcttgat gaatctagat ctagacttgg
     5581 agaaagactt agaattcatg cttctgctga tgatcttaga gctcttcttg ctagaccttg
     5641 gcttgaagga cttagagatc atcttcaatt tggagaacct gctgttgttc ctcatcctac
     5701 tccttataga caagtttcta gagttcaagc taagtctaat cctgaaagac ttagaagaag
     5761 attgatgagg agacatgatc tttctgaaga agaagctaga aagagaattc ctgatactgt
     5821 tgctagagct cttgatcttc cttttgttac tcttagatct caatctactg gacaacattt
     5881 tagacttttt attagacatg gacctcttca agttactgct gaagaaggag gatttacttg
     5941 ttatggactt tctaagggag gatttgttcc ttggttttga aaggtttgat gagtaccgga
     6001 atcaccaggg gaatcatatg aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct
     6061 tgtgtgctta agtttgtgtt tttttcttgg cttgttgtgt tatgaatttg tggctttttc
     6121 taatattaaa tgaatgtaag atctcattat aatgaataaa caaatgtttc tataatccat
     6181 tgtgaatgtt ttgttggatc tcttctgcag catataacta ctgtatgtgc tatggtatgg
     6241 actatggaat atgattaaag ataagtgagt gacctaagct agcctagtgt ctctgtctgt
     6301 gagacctctg gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     6361 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     6421 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
     6481 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagcct tcttctgaaa
     6541 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     6601 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     6661 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     6721 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     6781 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     6841 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     6901 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     6961 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     7021 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     7081 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     7141 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     7201 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     7261 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     7321 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     7381 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     7441 gccgcccgcg cacatctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     7501 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     7561 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     7621 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     7681 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     7741 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     7801 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     7861 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     7921 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     7981 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     8041 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     8101 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     8161 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     8221 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     8281 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     8341 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     8401 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     8461 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     8521 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     8581 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     8641 ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc
     8701 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     8761 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     8821 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     8881 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     8941 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     9001 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     9061 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     9121 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     9181 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     9241 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     9301 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     9361 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     9421 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     9481 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     9541 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     9601 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     9661 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     9721 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     9781 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     9841 gactctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     9901 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     9961 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
    10021 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
    10081 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
    10141 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
    10201 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
    10261 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacagt cacagcttgt
    10321 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    10381 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
    10441 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    10501 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
    10561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    10621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    10681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    10801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    10861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    11041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    11101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    11161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    11221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    11281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    11341 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
    11401 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
    11461 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
    11521 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
    11581 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
    11641 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
    11701 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
    11761 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
    11821 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
    11881 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
    11941 ttaacagcga tcgcgtattt cgtcttgctc aggcgcaatc acgaatgaat aacggtttgg
    12001 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
    12061 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
    12121 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
    12181 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
    12241 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
    12301 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
    12361 actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta
    12421 acgtgagtta cgcgtcgttc cactgagcgt cagacccctg ccggtaacat gagcaaagtc
    12481 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
    12541 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
    12601 tattgtggtg taaacaaatt gacgcttaga cggattacgg gtctcagcgg tacggcggga
    12661 tcatgagcgg agaattaagg gagtcacgtt atgacc
//
