LOCUS       sequence-444200-       13490 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Csy4 with 5' and 3' targets (I) and without Cas for Nicotiana leaf 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13490)
  AUTHORS   Bircheneder M, Schreiber T, Tissier A, Parniske M
  TITLE     A quantitative assay for the efficiency of RNA-guided genome editing
            in plants.
  JOURNAL   Plant J. 2024 Sep;119(5):2564-2577. doi: 10.1111/tpj.16931. Epub 
            2024 Jul 20.
  PUBMED    39032106
REFERENCE   2  (bases 1 to 13490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16931";
            journalName: "Plant J"; date: "2024-09"; volume: "119"; issue: "5"; 
            pages: "2564-2577"
COMMENT     Sequence Label: pMB23
FEATURES             Location/Qualifiers
     source          1..13490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..158
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     73..92
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(173..190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(344..932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(424..443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1002..1021)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1003..1021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1026..1042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1026..1042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1144..1163)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1155..1964
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1222..1241)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2220..2244
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2371..2550
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2403..2586
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5038..5055
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    5066..5085
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     terminator      6787..7039
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      9136..9384
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    9448..9472
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10773..11402
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11831..12904
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12970..13164
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13400..13422)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
       61 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
      121 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
      181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      781 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      961 ctgacgctca taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     1021 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     1081 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     1141 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     1201 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1261 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1321 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     1381 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     1441 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     1501 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     1561 attgtccttt taacagcgat cgcgtatttc gtcttgctca ggcgcaatca cgaatgaata
     1621 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     1681 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     1741 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     1801 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     1861 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     1921 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     1981 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     2041 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agacccctgc cggtaacatg
     2101 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
     2161 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     2221 ggcaggatat attgtggtgt aaacaaattg acgcttagac ggattacggg tctcagcggt
     2281 acggcgggat catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg
     2341 ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc
     2401 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca
     2461 aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac
     2521 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
     2581 tctgcaccgg atcttctgga agcaccatga cgagtaaagt gtatgatcct gagcaaagga
     2641 agagaatgat cacaggtcct caatggtggg ctaggtgtaa acagatgaat gttcttgact
     2701 ctttcatcaa ctactacgat tctgagaaac atgccgaaaa cgcagtgatt ttcctccatg
     2761 gaaatgctgc ttcaagctat ctctggagac acgttgtccc acatatagaa ccagtagcta
     2821 gatgcattat accggatttg attggtatgg gcaaaagtgg gaaatcaggg aatggatcct
     2881 acaggttatt ggaccactac aagtatctca cagcttggtt tgaactactt aacttgccta
     2941 agaagatcat attcgtggga catgattggg gagcatgtct tgcctttcac tattcctatg
     3001 aacatcagga caaaataaag gccattgtcc atgcagagtc tgttgttgac gtgattgaaa
     3061 gctgggatga atggcctgac attgaggagg atattgcgtt aatcaagagt gaagaaggcg
     3121 agaaaatggt tcttgaaaac aatttctttg tcgaaactat gttgccgtct aaaatcatga
     3181 gaaaactgga accagaagaa ttcgcagcat acttagaacc ctttaaggag aaaggagaag
     3241 tcagacgacc aactctgtct tggcctagag agattccatt ggttaagggt ggcaaaccag
     3301 atgttgtaca aattgtgcga aattataacg cttatctgcg tgcttcagat gatcttccta
     3361 agatgtttat tgagtcagat cccggtttct ttagcaatgc gattgtagag ggtgctaaga
     3421 aatttccaaa taccgaattc gtgaaggtta aaggactaca cttttctcaa gaagatgcac
     3481 ctgatgagat ggggaagtac ataaagtcct ttgtagagcg tgttcttaag aatgagcagt
     3541 aaaagggcta agacctaagc tagcctagaa tccggccatg ctagagtccg caaaaatcac
     3601 cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt
     3661 cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
     3721 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     3781 accaaaatcc agtgaccttg agtgacctaa gctagcctag tgtcgggcta gataattagg
     3841 ctaaactatc cttaaggatt gtagataatt aggctaaact atccttaagg tgtcgcgggg
     3901 agagaggatt ttgaggaaat aattaattga attacttgta ttattgataa agtaatttag
     3961 ataagttgtt agtacaactt attgcaacta tggatagaca aaaatcactt attttaaggg
     4021 gagaagtata taacaactta ttataaaatt tctgagcagc cggcctcctc caatcaatca
     4081 tagagagtgg agcccattct ggaaaaccaa ggaaccccca acttgcactg gtgcggtggc
     4141 ccaattcaaa aaaaacgggg cccagcaaag taacgccgtt gtaacgattt atcaatccaa
     4201 actcaaaagg cggcgagatg cgtttaactc cgtgaaatta acaaaccgcc aacaacttgc
     4261 aatttgcaac taccgtttcc aagaagaact caaccacaca acgtatccta tcccaaacca
     4321 cacgcaacta gtgacgcgtc ataaggacac gtgtcacaat ttgactggtt aataatttca
     4381 ccgcttttgc tataaattac ctccaatccc cttagcttct tcacaattca gttcccaacc
     4441 ctaacaattc ttgttcatat cgcttctctc tactttcaag gtatgatcca atttctctct
     4501 tctttctctg taatcctttc gttgagtttt gtttccgatc aatcataggt agttttcttg
     4561 tttcgaagca tgagatctag gaattttttg tgattttcca aaattgagat cggtttgaaa
     4621 ttgaatttta cagcttgaat ctcagatctt gttttatcaa tgttttcgat ggcttgcgat
     4681 gtagatctat gataattgtg gttcagtttt gttaggaatc gatttcggtt tagcaattgc
     4741 agattaatta gggtttccaa ttgaattctt cagatccgtt atggaattat gtcaaataat
     4801 ttattcaaat tggaaattat tgttagatcc actcttaatc tgtttgatcc aagcttcaat
     4861 tagggttttc acttgtttca atttcttgtt atggattctg atttatctgt tgatgttaga
     4921 tccactctta atctgtttga tccaagcgtt aattagggtt ttcacttgtt tcaatttcgt
     4981 gtgttggatt ctgatttatc tgttgttgat gtgattacag tctgaacaat gggcagccac
     5041 caccaccacc accacagcgg aatgagttca ctgccgtata ggcagctaag aaaatcaggt
     5101 ggcaccgaag atgcaaagaa cattaagaaa gggcctgcac cattctatcc acttgaggac
     5161 ggtacagccg gtgagcaatt gcataaggcc atgaagagat atgcacttgt accagggacg
     5221 atagccttta ctgatgctca cattgaggtc aatatcactt atgcggaata ttttgaaatg
     5281 tcagtcagat tggcagaagc tatgaaaagg tatggcttaa ataccaatca cagaattgtg
     5341 gtgtgttctg agaatagttt acagttcttc atgccagtgt tgggtgcact atttattggt
     5401 gtagctgttg ctccagctaa cgacatttac aatgagagag aactactcaa ctctatgaat
     5461 atttcacagc ccacagtcgt atttgtgagc aagaaaggat tgcagaagat acttaacgtt
     5521 cagaagaaac ttccgattat acagaagatc attattatgg acagcaaaac tgactaccaa
     5581 ggctttcagt ctatgtatac atttgtgaca tctcatttac ctccaggctt taatgagtat
     5641 gacttcgttc cagaatcatt tgatcgtgat aagactatcg ctcttattat gaatagctct
     5701 ggatccactg gtctgccgaa gggagtagca ttgcctcata ggacagcttg tgttaggttt
     5761 tcacacgcaa gagatccgat atttggaaat cagatcattc ctgatacggc tatattgagt
     5821 gttgtaccat ttcaccatgg ctttggaatg tttaccacac ttgggtactt gatttgcggc
     5881 tttagagtgg tactgatgta caggttcgag gaggaacttt tcctacgaag tctccaagat
     5941 tacaaaatcc aatccgcttt gttagttcct acactgtttt ccttcttcgc gaaatctacc
     6001 ctgattgaca aatatgatct ctctaatctc cacgaaatag caagtggtgg tgcacctctt
     6061 tcaaaagagg ttggagaagc agttgccaaa cgttttcatc taccaggtat cagacaagga
     6121 tatggtctta ctgagacaac atcagccata ttaattaccc ctgaaggcga tgataagcct
     6181 ggtgctgttg gaaaagttgt tccattcttc gaagcaaaag tggtagactt ggatactggc
     6241 aagacgcttg gagtcaacca aagaggtgaa ctgtgcgtta gagggcctat gatcatgtct
     6301 gggtacgtta acaatcctga ggcaaccaat gcgttaattg acaaagatgg ctggttacat
     6361 agtggagata ttgcgtactg ggacgaagat gagcacttct tcatagtgga taggttgaag
     6421 tctctcatca agtacaaagg ttatcaagta gctcctgctg aattagagtc aatccttctt
     6481 caacatccca acattttcga tgctggagtg gccggattgc ccgatgatga tgccggtgaa
     6541 ttgcccgcag ctgttgttgt gctggaacat ggtaagacta tgacagagaa ggaaatagtg
     6601 gactatgtcg ctagccaagt tactactgct aagaaactcc gtggaggggt tgtctttgtt
     6661 gatgaagtcc caaaaggatt gactgggaaa cttgatgctc gaaagataag agagattctc
     6721 attaaagcta agaagggtgg aaaatccaaa ctataaaagg gctaagacct aagctagcct
     6781 agaatcgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     6841 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     6901 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     6961 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     7021 tcatctatgt tactagatct gagtgaccta agctagccta gtgtctgagg cggccaacaa
     7081 actattcgtc acgtcgacaa aatttagaac gaacttaatt atgatctcaa atacattgat
     7141 acatatctca tctagatcta ggttatcatt atgtaagaaa gttttgacga atatggcacg
     7201 acaaaatggc tagactcgat gtaattggta tctcaactca acattatact tataccaaac
     7261 attagttaga caaaatttaa acaactattt tttatgtatg caagagtcag catatgtata
     7321 attgattcag aatcgttttg acgagttcgg atgtagtagt agccattatt taatgtacat
     7381 actaatcgtg aatagtgaat atgatgaaac attgtatctt attgtataaa tatccataaa
     7441 cacatcatga aagacacttt ctttcacggt ctgaattaat tatgatacaa ttctaataga
     7501 aaacgaatta aattacgttg aattgtatga aatctaattg aacaagccaa ccacgacgac
     7561 gactaacgtt gcctggattg actcggttta agttaaccac taaaaaaacg gagctgtcat
     7621 gtaacacgcg gatcgagcag gtcacagtca tgaagccatc aaagcaaaag aactaatcca
     7681 agggctgaga tgattaatta gtttaaaaat tagttaacac gagggaaaag gctgtctgac
     7741 agccaggtca cgttatcttt acctgtggtc gaaatgattc gtgtctgtcg attttaatta
     7801 tttttttgaa aggccgaaaa taaagttgta agagataaac ccgcctatat aaattcatat
     7861 attttcctct ccgctttgaa ttgtctcgtt gtcctcctca ctttcatcag ccgttttgaa
     7921 tctccggcga cttgacagag aagaacaagg aagaagtcta agagagaaag taagagataa
     7981 tccaggagat tcattctccg ttttgaatct tcctcaatct catcttcttc cgctctttct
     8041 ttccaaggta ataggaactt tctggatcta ctttatttgc tggatctcga tcttgttttc
     8101 tcaatttcct tgagatctgg aattcgttta atttggatct gtgaacctcc actaaatctt
     8161 ttggttttac tagaatcgat ctaagttgac cgatcagtta gctcgattat agctaccaga
     8221 atttggcttg accttgatgg agagatccat gttcatgtta cctgggaaat gatttgtata
     8281 tgtgaattga aatctgaact gttgaagtta gattgaatct gaacactgtc aatgttagat
     8341 tgaatctgaa cactgtttaa ggttagatga agtttgtgta tagattcttc gaaactttag
     8401 gatttgtagt gtcgtacgtt gaacagaaag ctatttctga ttcaatcagg gtttatttga
     8461 ctgtattgaa ctctttttgt gtgtttgcag ctcataaaaa tctgcccacg actcacatag
     8521 tccccctcaa acaccatgga tcattatctt gatattagac ttagacctga tcctgaattt
     8581 cctcctgctc aacttatgtc tgttcttttt ggaaagcttc atcaagctct tgttgctcaa
     8641 ggaggagata gaattggagt ttcttttcct gatcttgatg aatctagatc tagacttgga
     8701 gaaagactta gaattcatgc ttctgctgat gatcttagag ctcttcttgc tagaccttgg
     8761 cttgaaggac ttagagatca tcttcaattt ggagaacctg ctgttgttcc tcatcctact
     8821 ccttatagac aagtttctag agttcaagct aagtctaatc ctgaaagact tagaagaaga
     8881 ttgatgagga gacatgatct ttctgaagaa gaagctagaa agagaattcc tgatactgtt
     8941 gctagagctc ttgatcttcc ttttgttact cttagatctc aatctactgg acaacatttt
     9001 agacttttta ttagacatgg acctcttcaa gttactgctg aagaaggagg atttacttgt
     9061 tatggacttt ctaagggagg atttgttcct tggttttgaa aggtttgatg agtaccggaa
     9121 tcaccagggg aatcatatga agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt
     9181 gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt ggctttttct
     9241 aatattaaat gaatgtaaga tctcattata atgaataaac aaatgtttct ataatccatt
     9301 gtgaatgttt tgttggatct cttctgcagc atataactac tgtatgtgct atggtatgga
     9361 ctatggaata tgattaaaga taagtgagtg acctaagcta gcctagtgtc tctgtctgtg
     9421 agacctctgg tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     9481 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     9541 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     9601 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagcctt cttctgaaaa
     9661 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     9721 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     9781 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     9841 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     9901 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     9961 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
    10021 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
    10081 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
    10141 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
    10201 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
    10261 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
    10321 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
    10381 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
    10441 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
    10501 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
    10561 ccgcccgcgc acatctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
    10621 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
    10681 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
    10741 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
    10801 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
    10861 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
    10921 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
    10981 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
    11041 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
    11101 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
    11161 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
    11221 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
    11281 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
    11341 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
    11401 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
    11461 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
    11521 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
    11581 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
    11641 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
    11701 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
    11761 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg
    11821 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
    11881 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
    11941 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
    12001 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
    12061 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
    12121 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
    12181 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
    12241 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
    12301 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
    12361 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
    12421 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
    12481 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
    12541 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
    12601 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
    12661 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
    12721 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
    12781 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
    12841 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
    12901 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
    12961 actctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
    13021 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
    13081 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
    13141 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
    13201 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
    13261 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
    13321 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
    13381 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacagtc acagcttgtc
    13441 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
//
