LOCUS       sequence-44421-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Kakirde KS, Wild J, Godiska R, Mead DA, Wiggins AG, Goodman RM, 
            Szybalski W, Liles MR
  TITLE     Gram negative shuttle BAC vector for heterologous expression of 
            metagenomic libraries.
  JOURNAL   Gene. 2011 Apr 15;475(2):57-62. Epub 2010 Nov 26.
  PUBMED    21112378
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2011-04-15"; volume: "475(2)"; pages: "57-62. Epub 2010 Nov 
            26"
COMMENT     Sequence Label: pGNS-BAC
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..37
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     terminator      complement(108..135)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        194..212
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        286..731
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 atgactgatc gccaagctat ttaggtgaca ctatagaata ctcaattctc tgtccttcct
       61 gtgcgacggt tacgccgctc catgagctta tcgggtccac tcgcgaacgc agaaaggccc
      121 acccgaaggt gagccagtgt gattacattt gcggcctaac tgtggccagt ccagttacgc
      181 tggagtcact agtatttagg tgacactata gaagcggccg cgacaacttg tccacgtgga
      241 attctaagct tcggatccca ctgtggtgga tgcattttaa cccatcacat atacctgccg
      301 ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc
      361 aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag
      421 attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa
      481 agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta
      541 aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt
      601 gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact
      661 tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg
      721 agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta
      781 ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat
      841 ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac
      901 agcaaaaaaa tgaaaaatat caagttcct
//
