LOCUS       sequence-444211-        9518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression vector of eGFP tagged mouse MMS22L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   O'Donnell L, Panier S, Wildenhain J, Tkach JM, Al-Hakim A, Landry 
            MC, Escribano-Diaz C, Szilard RK, Young JT, Munro M, Canny MD, Kolas
            NK, Zhang W, Harding SM, Ylanko J, Mendez M, Mullin M, Sun T, 
            Habermann B, Datti A, Bristow RG, Gingras AC, Tyers MD, Brown GW, 
            Durocher D
  TITLE     The MMS22L-TONSL complex mediates recovery from replication stress 
            and homologous recombination.
  JOURNAL   Mol Cell. 2010 Nov 24;40(4):619-31. doi: 
            10.1016/j.molcel.2010.10.024. Epub 2010 Nov 4.
  PUBMED    21055983
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2010.10.024"; journalName: "Mol Cell"; date: 
            "2010-11-24- 24"; volume: "40"; issue: "4"; pages: "619-31"
COMMENT     Sequence Label: pcDNA5-FRT/TO-eGFP-msMMS22L
FEATURES             Location/Qualifiers
     source          1..9518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1035..1056)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1296..1315)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1643..1664
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(2944..2955)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     primer_bind     complement(5470..5487)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5476..5700
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5833..5852)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5984..6031
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6039..7061
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7191..7312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7228..7247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7282..7301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7361..7377)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7361..7377)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7374..7396)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7385..7401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7409..7415,7416..7433,7434..7439))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7454..7475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7592..7609)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7763..8351)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7843..7862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8522..9313,9314..9382))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9145..9164
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9383..9487)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggtgagcaa gggcgaggag ctgttcaccg
     1021 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1081 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1141 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1201 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1261 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1321 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1381 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1441 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1501 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1561 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1621 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1681 ctctcggcat ggacgagctg tacaagggcg cgccaatgga tggctgttca gcagcgtcga
     1741 ccttcttgac tgacagctta gagctggagc tggggacaga gtggtgcaaa cccccctgct
     1801 tttcttgcgc ttttgacaac agagaaggaa aattctctgg agagtcctac cttgccagtg
     1861 gcgcccttaa gcggttaatt ttaaatcttg atccattacc aactaatttt gaagaggata
     1921 cagtagaact atttggcttc caatgggtta cagaaacagc gctagtctat tcctgtagag
     1981 agctctttca tttattcagg caacagatat ttaacttgga gtccttggta caagtcagct
     2041 gtgattttgg gaaaatagcc accttgcacg ccaaagcaga cagtattagg caacaatgtg
     2101 tagtgttcct tcactacatt aaagttttca tcttcaggtg tctgaaagta caggaagcag
     2161 agagccacag tcgccctgcc catccctatg aggctttgga agcccagctt ccctccatgt
     2221 tggtcgatga gctccgtgga ttacttctgt acattggaca tctagctgca cttcccagtg
     2281 ttactgtagg agcgtttgta aatcaaaacc agatgaagct cttccctcca tcatggcatt
     2341 tattacatct ctatttagat acccactggc tggtgttgga aatactgcat attctgggag
     2401 aaaaattgaa acaagttgtt tatggtcgtc agtttattgg tcaggcaggt gacaatttaa
     2461 ccaatgtcag cctctttgaa gaacattgtg aacatctttt ctgtgactta atatgcctgt
     2521 cactcaacag atttgacaag gtcatgcctt cagaagcact actgattagt cactgcccat
     2581 gctcctgtgt taaagaatta tgggttctac ttattcatct tttagaccac agaaggaaat
     2641 ggtctgttgc agattcattt tggaattggt tgaataaact acttagaaca ctttttgaaa
     2701 agtcaagtga ccaacgaaga tcttctgtgt ctctaaccca ggccaaggat ccattaggct
     2761 ttagttggtg gattagtgtt catgtagcat cattgtacca gattgatcgc catggtgtgt
     2821 cagacaaaat gaaacagatg gaatccaatt ggagctttat agaagaactt ttgaaacggt
     2881 ccgtcactgt tcaggatagt atactagaag aacaattgcg aatgcatctt cattgttgtt
     2941 tgacactttg tgatttctgg gagccaaata tttcagttgt caccatttta tgggagtatt
     3001 attctaagaa cctgaatagc tccttcagta tttcctggct tcctttgaaa ggccttacca
     3061 atattatcaa gtctccactg tcgatgctta cactggtgag gaactgctgt agtgataaac
     3121 aagaccctga cctctataag tccagcagca gttacatcat ttttctttgc attctggcaa
     3181 aagttgttaa gaaagcaatg agaaccagcg gccctcaccc ttggaagcaa gttaaaggaa
     3241 gaatatattc taagtttcat caaaaaagaa tggaagaact aactgaagtt ggcctacaga
     3301 actttttcag cctttttcta ctgttagcag ctgttgcaga gatagaagat gtagcaagcc
     3361 atgttttaga cctccttcgt ttcctcaggc ctgcctccat gtcatctcat ggagcccttg
     3421 tttggaaggg tcagatggca tttctcctga tgtatgccca gaaaaacttg gacattggtg
     3481 tttgggctga gaaactgtca tgtgaattcc aagagaaagc aaaggaattc ttggtatcta
     3541 agaatgatga aatggtacag agacatgctc tatggacact gctttgcatc tatattgatg
     3601 gtgttcaaga agtatttgag accagcagtt gcttgtatcc ttctcatgag catcttctca
     3661 atgatggatt tagcatgtta ctgccagcct gtcgagaatc tgaacttagg acagtcctga
     3721 actttctaca agctgttctg gcgagaatca ggagtgttca tcagcaattg tgtcaggaac
     3781 ttcaaagaga gaatgtggac ctaactgtgc agtcttcatt atcggctaaa gagcgccccc
     3841 tggctgccgt cgctggtgca ctgtggaggc atttcttttc ctttctcaag agtcagagaa
     3901 tgacacaagt tgtgcctctc tcccagcttg cagatgccgc tgcagatttt actttgctgg
     3961 cagtggacat gccaaacaca gcaccaccag atcttcagcc acagccagtt atatcaataa
     4021 ttcagctttt tggttgggat gatatcatct ggccccaagt tgtagcaaga tatttaagtc
     4081 atttgctaca aaatagcaca gtatatgaag cactttctca gtcaagttgc gtgtcttctc
     4141 agtccttaac tataagatca tgggtccgtt gtgttttgca aatgcacata aaacacctct
     4201 ctgaccctga cttgctgatt gatgtgaatc ctgaacaggc cgtggaaaaa gagtacatgg
     4261 aacagctggc tgaaatgaca agattactat ttacactctc agaagtaaag agtgtcttct
     4321 caaaggctca aatagagcaa ttacccagcc cagacgatcc caagcaagcg cttattcagt
     4381 tcctggaggc tgttggtgtg acttacagga ctctgcagac attttctgat aaatctgcca
     4441 tggtcacaaa gtccttagaa taccttggtg aagtattaaa atatataaag ccttatttgg
     4501 ggaaaaaagt ttccagtgca gggttgcagc tgacttacgg gataatggga attcttgtta
     4561 agtcatgggc acacatcttt gccacctcta aagcacagaa gctacttttt cggataatag
     4621 actgtttgtt gctgccacat acagtgctac agcaagacaa ggagctgcct gggcccatgc
     4681 tgactgcaat tcagaagacg cttcctctct atctacaggg catatgtatt gtatgctgcc
     4741 agtctcaaaa tccaaatgcc tacttgaatc agttgctgag gaatgtcatt gagcagtaca
     4801 tagggaggtt tctcccaacc tcgccctgtg tttcagatct gggacagcac cctgttctgt
     4861 tggcactgag aaaccctgcc tctgttccct cgatgacgcc tctaagaaag cacactgtcc
     4921 atgccataag gaaatcctac cttgagttca aagggtcctc accccctcct cgattagcat
     4981 ccgtattggc cttcgtcctt cagctcttca aggacaccga aatgggtgcc tgtgaccttg
     5041 agctgctcct tcctggcatc ctgaaatgtt tggtgttggt aaatgaaccc caagttaaaa
     5101 agctggccac agagaacctg cagtgcatgg tacaaacctg ccaagtgggg tcggaaggag
     5161 gacctgccac ccagctgact tctctgttta ggcagttcat ccaggactat ggtatgcaat
     5221 acagttatca agtatacagc atcctagaga cagtagcaac gctgaatcag cacgttgtca
     5281 tccaattgat tcctaccctc actcagtctc tgaaggattc agagctcaaa tggggcctcg
     5341 gcagaaatat tgcacaaagg gaagcatata gcagactttt atctggccta ggacaggtag
     5401 gacagggcga gaagcagaga ctggaaaagt agctcgagtc tagagggccc gtttaaaccc
     5461 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5521 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5581 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     5641 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     5701 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     5761 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5821 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5881 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5941 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa
     6001 gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga
     6061 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     6121 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     6181 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     6241 cggccgcgct cccgattccg gaagtgcttg acattgggga gttcagcgag agcctgacct
     6301 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     6361 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     6421 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     6481 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     6541 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     6601 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     6661 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     6721 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     6781 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     6841 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     6901 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     6961 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     7021 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg
     7081 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7141 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7201 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7261 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7321 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7381 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7441 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7501 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7561 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7621 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7681 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7741 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7801 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7861 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7921 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7981 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8041 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8101 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8161 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8221 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8281 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8341 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8401 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8461 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8521 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8581 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8641 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8701 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8761 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8821 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8881 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8941 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9001 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9061 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9121 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9181 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9241 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9301 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9361 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9421 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9481 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
