LOCUS       sequence-444212-        8843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression vector of Flag tagged mouse MMS22L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   O'Donnell L, Panier S, Wildenhain J, Tkach JM, Al-Hakim A, Landry 
            MC, Escribano-Diaz C, Szilard RK, Young JT, Munro M, Canny MD, Kolas
            NK, Zhang W, Harding SM, Ylanko J, Mendez M, Mullin M, Sun T, 
            Habermann B, Datti A, Bristow RG, Gingras AC, Tyers MD, Brown GW, 
            Durocher D
  TITLE     The MMS22L-TONSL complex mediates recovery from replication stress 
            and homologous recombination.
  JOURNAL   Mol Cell. 2010 Nov 24;40(4):619-31. doi: 
            10.1016/j.molcel.2010.10.024. Epub 2010 Nov 4.
  PUBMED    21055983
REFERENCE   2  (bases 1 to 8843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2010.10.024"; journalName: "Mol Cell"; date: 
            "2010-11-24- 24"; volume: "40"; issue: "4"; pages: "619-31"
COMMENT     Sequence Label: pcDNA5-FRT/TO-FLAG-msMMS22L
FEATURES             Location/Qualifiers
     source          1..8843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             993..1016
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2269..2280)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     primer_bind     complement(4795..4812)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4801..5025
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5158..5177)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5309..5356
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5364..6386
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6516..6637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6553..6572)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6607..6626
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6686..6702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6686..6702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6699..6721)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6710..6726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6734..6740,6741..6758,6759..6764))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6779..6800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6917..6934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7088..7676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7168..7187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7847..8638,8639..8707))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8470..8489
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8708..8812)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggactacaa agacgatgac gataaagccg
     1021 ccagtttaaa cggcgcgcca atggatggct gttcagcagc gtcgaccttc ttgactgaca
     1081 gcttagagct ggagctgggg acagagtggt gcaaaccccc ctgcttttct tgcgcttttg
     1141 acaacagaga aggaaaattc tctggagagt cctaccttgc cagtggcgcc cttaagcggt
     1201 taattttaaa tcttgatcca ttaccaacta attttgaaga ggatacagta gaactatttg
     1261 gcttccaatg ggttacagaa acagcgctag tctattcctg tagagagctc tttcatttat
     1321 tcaggcaaca gatatttaac ttggagtcct tggtacaagt cagctgtgat tttgggaaaa
     1381 tagccacctt gcacgccaaa gcagacagta ttaggcaaca atgtgtagtg ttccttcact
     1441 acattaaagt tttcatcttc aggtgtctga aagtacagga agcagagagc cacagtcgcc
     1501 ctgcccatcc ctatgaggct ttggaagccc agcttccctc catgttggtc gatgagctcc
     1561 gtggattact tctgtacatt ggacatctag ctgcacttcc cagtgttact gtaggagcgt
     1621 ttgtaaatca aaaccagatg aagctcttcc ctccatcatg gcatttatta catctctatt
     1681 tagataccca ctggctggtg ttggaaatac tgcatattct gggagaaaaa ttgaaacaag
     1741 ttgtttatgg tcgtcagttt attggtcagg caggtgacaa tttaaccaat gtcagcctct
     1801 ttgaagaaca ttgtgaacat cttttctgtg acttaatatg cctgtcactc aacagatttg
     1861 acaaggtcat gccttcagaa gcactactga ttagtcactg cccatgctcc tgtgttaaag
     1921 aattatgggt tctacttatt catcttttag accacagaag gaaatggtct gttgcagatt
     1981 cattttggaa ttggttgaat aaactactta gaacactttt tgaaaagtca agtgaccaac
     2041 gaagatcttc tgtgtctcta acccaggcca aggatccatt aggctttagt tggtggatta
     2101 gtgttcatgt agcatcattg taccagattg atcgccatgg tgtgtcagac aaaatgaaac
     2161 agatggaatc caattggagc tttatagaag aacttttgaa acggtccgtc actgttcagg
     2221 atagtatact agaagaacaa ttgcgaatgc atcttcattg ttgtttgaca ctttgtgatt
     2281 tctgggagcc aaatatttca gttgtcacca ttttatggga gtattattct aagaacctga
     2341 atagctcctt cagtatttcc tggcttcctt tgaaaggcct taccaatatt atcaagtctc
     2401 cactgtcgat gcttacactg gtgaggaact gctgtagtga taaacaagac cctgacctct
     2461 ataagtccag cagcagttac atcatttttc tttgcattct ggcaaaagtt gttaagaaag
     2521 caatgagaac cagcggccct cacccttgga agcaagttaa aggaagaata tattctaagt
     2581 ttcatcaaaa aagaatggaa gaactaactg aagttggcct acagaacttt ttcagccttt
     2641 ttctactgtt agcagctgtt gcagagatag aagatgtagc aagccatgtt ttagacctcc
     2701 ttcgtttcct caggcctgcc tccatgtcat ctcatggagc ccttgtttgg aagggtcaga
     2761 tggcatttct cctgatgtat gcccagaaaa acttggacat tggtgtttgg gctgagaaac
     2821 tgtcatgtga attccaagag aaagcaaagg aattcttggt atctaagaat gatgaaatgg
     2881 tacagagaca tgctctatgg acactgcttt gcatctatat tgatggtgtt caagaagtat
     2941 ttgagaccag cagttgcttg tatccttctc atgagcatct tctcaatgat ggatttagca
     3001 tgttactgcc agcctgtcga gaatctgaac ttaggacagt cctgaacttt ctacaagctg
     3061 ttctggcgag aatcaggagt gttcatcagc aattgtgtca ggaacttcaa agagagaatg
     3121 tggacctaac tgtgcagtct tcattatcgg ctaaagagcg ccccctggct gccgtcgctg
     3181 gtgcactgtg gaggcatttc ttttcctttc tcaagagtca gagaatgaca caagttgtgc
     3241 ctctctccca gcttgcagat gccgctgcag attttacttt gctggcagtg gacatgccaa
     3301 acacagcacc accagatctt cagccacagc cagttatatc aataattcag ctttttggtt
     3361 gggatgatat catctggccc caagttgtag caagatattt aagtcatttg ctacaaaata
     3421 gcacagtata tgaagcactt tctcagtcaa gttgcgtgtc ttctcagtcc ttaactataa
     3481 gatcatgggt ccgttgtgtt ttgcaaatgc acataaaaca cctctctgac cctgacttgc
     3541 tgattgatgt gaatcctgaa caggccgtgg aaaaagagta catggaacag ctggctgaaa
     3601 tgacaagatt actatttaca ctctcagaag taaagagtgt cttctcaaag gctcaaatag
     3661 agcaattacc cagcccagac gatcccaagc aagcgcttat tcagttcctg gaggctgttg
     3721 gtgtgactta caggactctg cagacatttt ctgataaatc tgccatggtc acaaagtcct
     3781 tagaatacct tggtgaagta ttaaaatata taaagcctta tttggggaaa aaagtttcca
     3841 gtgcagggtt gcagctgact tacgggataa tgggaattct tgttaagtca tgggcacaca
     3901 tctttgccac ctctaaagca cagaagctac tttttcggat aatagactgt ttgttgctgc
     3961 cacatacagt gctacagcaa gacaaggagc tgcctgggcc catgctgact gcaattcaga
     4021 agacgcttcc tctctatcta cagggcatat gtattgtatg ctgccagtct caaaatccaa
     4081 atgcctactt gaatcagttg ctgaggaatg tcattgagca gtacataggg aggtttctcc
     4141 caacctcgcc ctgtgtttca gatctgggac agcaccctgt tctgttggca ctgagaaacc
     4201 ctgcctctgt tccctcgatg acgcctctaa gaaagcacac tgtccatgcc ataaggaaat
     4261 cctaccttga gttcaaaggg tcctcacccc ctcctcgatt agcatccgta ttggccttcg
     4321 tccttcagct cttcaaggac accgaaatgg gtgcctgtga ccttgagctg ctccttcctg
     4381 gcatcctgaa atgtttggtg ttggtaaatg aaccccaagt taaaaagctg gccacagaga
     4441 acctgcagtg catggtacaa acctgccaag tggggtcgga aggaggacct gccacccagc
     4501 tgacttctct gtttaggcag ttcatccagg actatggtat gcaatacagt tatcaagtat
     4561 acagcatcct agagacagta gcaacgctga atcagcacgt tgtcatccaa ttgattccta
     4621 ccctcactca gtctctgaag gattcagagc tcaaatgggg cctcggcaga aatattgcac
     4681 aaagggaagc atatagcaga cttttatctg gcctaggaca ggtaggacag ggcgagaagc
     4741 agagactgga aaagtagctc gagtctagag ggcccgttta aacccgctga tcagcctcga
     4801 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4861 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4921 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4981 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5041 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5101 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5161 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5221 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5281 ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc tattctctag
     5341 aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     5401 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     5461 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     5521 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     5581 ttccggaagt gcttgacatt ggggagttca gcgagagcct gacctattgc atctcccgcc
     5641 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     5701 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     5761 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     5821 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     5881 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     5941 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     6001 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     6061 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     6121 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     6181 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     6241 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     6301 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     6361 gcactcgtcc gagggcaaag gaatagcacg tactacgaga tttcgattcc accgccgcct
     6421 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     6481 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     6541 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6601 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     6661 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6721 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6781 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6841 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6901 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6961 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7021 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7081 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7141 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7201 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7261 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7321 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7381 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7441 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7501 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7561 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7621 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7681 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7741 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7801 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7861 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7921 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7981 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8041 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8101 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8161 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8221 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8281 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8341 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8401 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8461 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8521 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8581 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8641 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8701 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8761 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8821 cccgaaaagt gccacctgac gtc
//
