LOCUS       sequence-444221-        3217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of MAG transposase 
            mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pICOZ-MAG-M235Q
FEATURES             Location/Qualifiers
     source          1..3217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..251
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     202..222
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     296..315
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        296..314
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      380..389
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1425..1443)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1426..1443)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1463..1480)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1469..1693
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1696..1712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1696..1712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(1758..2346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1838..1857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2371..2745)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2746..2808)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2845..2862
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2846..2862
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        join(2885..3217,1..47)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
       61 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      121 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      181 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      241 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
      301 cgactcacta tagggagacc caagcttggt accgagctcg gatccactag taacggccgc
      361 cagtgtgctg gaattcgccg ccaccatggg tcgttcagcg catagcaccc aacaacagcg
      421 cctggacatt aaacgcttaa gcgccgccgg ttatagccaa cgcaaaattg cggaaatcct
      481 gggccgcagc aagacctttg tgtataacgc cctgcatagc accggcacca aaattcctac
      541 tggccgccct cgtaaaacta gcgcgcgtga tgatgcacgt atgacccgtc tgtgtaaagc
      601 cgacccgttt aaaagcgcgc gcgccattcg tgatgaactg cagctgagcg ttagcgatcg
      661 caccgttcaa cgtcgcctgt tcgagaataa tctggtgggc cgcaatcctc gtaaagtgcc
      721 tctgctgcgt cgttgtcatg tgcaggcacg cttacaattt gcgcgcgagc actatgattg
      781 ggcgggcgag aacttaaaca aatggcgcaa cgtgctgtgg agcgatgaga gcaaagtgaa
      841 cctggtgggc agcgatggca aacgttttgt gcgccgccct aagaataccg cgtatcgccc
      901 tcagtatacc ctgaaaaccg tgaaacatgg cggcggcaac attatggtgt gggcgtgctt
      961 ctcatggtat ggcgtgggcc ctatcttctg gatcaaagac atcatggatc agcatcgcta
     1021 cctgaacatt attcagaccg tgatgctgcc tcacgccgaa tgggaaatgc ctctgaagtg
     1081 gcagttccag catgataacg acccgaagca taccgccaaa gcggtgaaga aatggttcgt
     1141 ggaccagaag attgatgtga tgaactggcc tgcgcagagc cctgatctga acccgatcga
     1201 aaacctgtgg aagattgtga aggccaagct gcctcctgca ggttcacgca ccaaagagaa
     1261 actgtggaag catattgaaa acgcgtggta cagcatacct ccgagcacct gtaaaagcct
     1321 ggtggaaagc atgccgaaac gtatgcgcgc ggtgattcgc aataacggcc atgcgaccaa
     1381 atattgatga gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta
     1441 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1501 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1561 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1621 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1681 gtgggctcta tggcggtcat agctgtttcc tgtgtgggat atcctcgaga cagtgggccc
     1741 gtttaaacac atgtgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1801 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1861 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1921 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1981 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2041 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2101 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2161 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatccgcgct
     2221 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaaaaaacc
     2281 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag gaaaaaagga
     2341 tctcaagaag atcctttgat cttttctacg tcagtcctgc tcctcggcca cgaagtgcac
     2401 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     2461 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     2521 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     2581 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     2641 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     2701 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatactct tccttttcaa
     2761 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacataac gcgtcgcgag
     2821 gccatatggg ttaacccatg gccttgtaaa acgacggcca gtcgacgggc cagatatacg
     2881 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     2941 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3001 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3061 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3121 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3181 gcctggcatt atgcccagta catgacctta tgggact
//
