LOCUS       sequence-444222-        3217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of MAG transposase 
            mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pICOZ-MAG-H181A
FEATURES             Location/Qualifiers
     source          1..3217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        100..479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(502..519)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(502..518)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        556..618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             619..993
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      1018..1606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1507..1526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1652..1668
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1652..1668
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1671..1895
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1884..1901
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        1921..1939
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1921..1938
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2975..2984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3049..3068)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3050..3068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(3113..3217,1..99))
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3142..3162)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
       61 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
      121 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
      181 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
      241 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
      301 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
      361 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
      421 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca
      481 acgcgtatat ctggcccgtc gactggccgt cgttttacaa ggccatgggt taacccatat
      541 ggcctcgcga cgcgttatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      601 atattgaaaa ggaagagtat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
      661 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
      721 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
      781 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
      841 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
      901 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
      961 tgcgtgcact tcgtggccga ggagcaggac tgacgtagaa aagatcaaag gatcttcttg
     1021 agatcctttt ttcctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1081 ggtggttttt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1141 cagagcgcgg ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1201 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1261 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1321 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1381 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1441 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1501 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1561 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaaca catgtgttta
     1621 aacgggccca ctgtctcgag gatatcccac acaggaaaca gctatgaccg ccatagagcc
     1681 caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc
     1741 caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt
     1801 ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg
     1861 gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc gagctctagc
     1921 atttaggtga cactatagaa tagggccctc tagatgcatg ctcgagcggc cgctcatcaa
     1981 tatttggtcg catggccgtt attgcgaatc accgcgcgca tacgtttcgg catgctttcc
     2041 accaggcttt tacaggtgct cggaggtatg ctgtaccacg cgttttcaat atgcttccac
     2101 agtttctctt tggtgcgtga acctgcagga ggcagcttgg ccttcacaat cttccacagg
     2161 ttttcgatcg ggttcagatc agggctctgc gcaggccagt tcatcacatc aatcttctgg
     2221 tccacgaacc atttcttcac cgctttggcg gtatgcttcg ggtcgttatc atgcatgaac
     2281 tgccacttca gaggcatttc ccattcggcg tgaggcagca tcacggtctg aataatgttc
     2341 aggtagcgat gctgatccat gatgtctttg atccagaaga tagggcccac gccataccat
     2401 gagaagcacg cccacaccat aatgttgccg ccgccagctt tcacggtttt cagggtatac
     2461 tgagggcgat acgcggtatt cttagggcgg cgcacaaaac gtttgccatc gctgcccacc
     2521 aggttcactt tgctctcatc gctccacagc acgttgcgcc atttgtttaa gttctcgccc
     2581 gcccaatcat agtgctcgcg cgcaaattgt aagcgtgcct gcacatgaca acgacgcagc
     2641 agaggcactt tacgaggatt gcggcccacc agattattct cgaacaggcg acgttgaacg
     2701 gtgcgatcgc taacgctcag ctgcagttca tcacgaatgg cgcgcgcgct tttaaacggg
     2761 tcggctttac acagacgggt catacgtgca tcatcacgcg cgctagtttt acgagggcgg
     2821 ccagtaggaa ttttggtgcc ggtgctatgc agggcgttat acacaaaggt cttgctgcgg
     2881 cccaggattt ccgcaatttt gcgttggcta taaccggcgg cgcttaagcg tttaatgtcc
     2941 aggcgctgtt gttgggtgct atgcgctgaa cgacccatgg tggcggcgaa ttccagcaca
     3001 ctggcggccg ttactagtgg atccgagctc ggtaccaagc ttgggtctcc ctatagtgag
     3061 tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc
     3121 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     3181 tacgacattt tggaaagtcc cgttgatttt ggtgcca
//
