LOCUS       sequence-444223-        8734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of piggyBac-1_AMi Transposon with 5 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-piggyBac-1_AMi-5 kb
FEATURES             Location/Qualifiers
     source          1..8734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    562..643
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     587..606
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1187..1203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1187..1203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1215..1232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1386..1974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1466..1485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2145..2936,2937..3005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     2768..2787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3006..3110)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3178..3196
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3205..3222
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3206..3222
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4304..4313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4329..4828
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4364..4387)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4761..4780
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4842..4851
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4848..5447
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5347..5367
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5454..5510
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5511..5513,5514..5516,5517..6230)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5511..5513,5514..5516,5517..6227)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5556..5577)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5817..5836)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6164..6185
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6252..6269)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6258..6482
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6528..7706
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6759..7697
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7654..7674
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7721..7730
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7730..7753
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtggtggttg ggggagtcct ggcttgctat agcttgctag taacagtggc ctttattatt
       61 ttctgggtga ggagtaagag gagcaggctc ctgcacagtg actacatgaa catgactccc
      121 cgccgccccg ggcccacccg caagcattac cagccctatg ccccaccacg cgacttcgca
      181 gcctatcgct ccagagtgaa gttcagcagg agcgcagacg cccccgcgta ccagcagggc
      241 cagaaccagc tctataacga gctcaatcta ggacgaagag aggagtacga tgttttggac
      301 aagagacgtg gccgggaccc tgagatgggg ggaaagccga gaaggaagaa ccctcaggaa
      361 ggcctgtaca atgaactgca gaaagataag atggcggagg cctacagtga gattgggatg
      421 aaaggcgagc gccggagggg caaggggcac gatggccttt accagggtct cagtacagcc
      481 accaaggaca cctacgacgc ccttcacatg caggccctgc cccctcgcat cgattgagtt
      541 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      601 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      661 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      721 ctacaaatgt ggtaccttct gaagcgcttg tacacatatg gtcgacctgc agaattcact
      781 agtggcgcgc cctcgagtta aaacagttag gaaatgtttt tggaagtaat tgttagtttg
      841 aaaacagtgt gaactgcaga cttagcaaat tgttcattca cgttttgaaa ctctaaaaaa
      901 agtttaaatt ttgttcatag ttctagttaa ataatatttt gggttcaaat catcataaat
      961 actctttcaa acaatctgta tgtttaataa atagtttttg gtacatttat actgttgtca
     1021 ctggttccat tttgttcatg cgggtggggt aacaagttac cccatgagac caacaatgtc
     1081 gaattgcggg gtaacaagtt accccatgag aacaacaacg gttacggttt catgagaacg
     1141 gatgagggtt aagggcccag agacggagtc actgccaacc gagacggtca tagctgtttc
     1201 ctgtgtgccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1261 gtatcagctc actcaaaggc ggtaatacgg ttacccacag aatcagggga taacgcagga
     1321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1561 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1801 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1861 gttaccttcg gaaaaagagt tggtagctct tgatcgggca aacaaaccac cgctggtagc
     1921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1981 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2041 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2161 agtgaggcac ctatctcagc gatctgtctc tttcgttcat ccatagttgc ctgactcccc
     2221 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaataata
     2281 ccgcgggacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2341 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2401 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct
     2461 acaggcatcg tggtatcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2521 cgatcaaggc gagttacatg atcccccatg ttgcgcaaaa aagcggttag ctccttcggt
     2581 cctccgatcg ttgtcagaag taagttggcc gccgtgttat cactcatggt tatggcagca
     2641 ctacataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2701 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2761 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2821 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2881 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2941 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3001 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3061 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3121 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     3181 aggcgtatca cgaggccctt tcgttgtaaa acgacggcca gtcgaaccac gcaatgcgtc
     3241 tcgatccgca gtgtcttgcg tctctcaatt gttaaccttc atacgttccc atggggtaat
     3301 ttcctacccc agacgcatat tttgtgcagt atcttaaagt taattaattc taaaaaatat
     3361 tctttttagg acattgtcac tggcaccgca gtgcacaata tccccaaaga acagccagct
     3421 tacatgaatg gtttgctctc tgtggatgaa aaactgccca gggtaacatg ttaccccagg
     3481 cgtgaatgtg tttttccata tggggtaaca acttacccca agcccatgtt aacgcattgg
     3541 ggtaacttgt taccccaatg caatttctgt ataaatgaca ggcctagacc tgtttggcca
     3601 ctttgggtgg atgcatctga aaggtcagca ttcattcaca cctcattcat gtccacttca
     3661 ggtatccatg gtaactaatg gatggccatc ctccactctg cttttgcaga agatgtggta
     3721 atcccttttg ctaaccactt tgtttgcagg ctgcaaggtc atcaaaacac cctgcccttt
     3781 gccaaaaaaa cccaaaaccc tttgtcacac ccaaatccac ctctgctctc ttttctcatg
     3841 gcaaaactcc gcctcacctg cccctttcat aacatacaag ctagtttgcc ctgcctttgt
     3901 tggaagtgag gacaaggagc aaagggacag cagagaagaa atagaacaac atcatactgc
     3961 aatacagcgt acactatgga gtccccttca acggtaaggg cttgacaccc cccatgcccc
     4021 ttttacctgc tctcaataag cccctgcatg tgtggtttcc aaacagcatc cacccacatt
     4081 tggcttgcct gcaaagggtg gatgctggtc tctggcttct gtgcccagga ggagagggtg
     4141 ctacctgcgc tgccataggg cagggcggaa ttgcttctgt gacagcatag gtggacaggc
     4201 ttgtctttga ttgttattta tatgcattca gcagtaatca tttttttttt ttttttctgc
     4261 agtccaggac cggtgctagc gggcccgacg tcgcatgctc ccggccgcca tgggtataca
     4321 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     4381 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     4441 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     4501 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     4561 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     4621 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     4681 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
     4741 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     4801 ctcctcttcc tcatctccgg gcctttcgac ctgcagccaa cgccaccatg gggaccgagt
     4861 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     4921 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     4981 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     5041 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     5101 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     5161 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     5221 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     5281 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     5341 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     5401 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccatc gatgccacta
     5461 acttctccct gttgaaacaa gcaggggatg tcgaagagaa tcccgggcca atggtgagca
     5521 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     5581 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     5641 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     5701 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     5761 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5821 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5881 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5941 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     6001 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     6061 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     6121 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     6181 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ttaattaagc
     6241 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6301 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6361 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6421 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     6481 ggcttctgag ggcggccgcc aatgggtacc ttggttaatt aacgtgaggc tccggtgccc
     6541 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     6601 attgacccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     6661 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     6721 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     6781 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     6841 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     6901 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     6961 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     7021 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     7081 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     7141 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     7201 gaatcggacg ggggtagtct caagctcgcc ggcctgctct ggtgcctggc ctcgcgccgc
     7261 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     7321 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     7381 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     7441 cttcatgtga ctccactgag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     7501 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     7561 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     7621 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     7681 tttttcttcc atttcaggtg tcgtgaggga tccaccggtc gccaccatgg attacaaaga
     7741 cgatgacgat aaggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca
     7801 cgccgccagg ccgcaagtgc aactccaaca atctggcccc ggtctggtca ctccttccca
     7861 gactcttagt cttacctgtg ccatatcagg tgattcagtg agtagtaact ccgcgacatg
     7921 gaattggatc aggcagtccc ccagtcgagg gttggaatgg ctcggtagaa catactaccg
     7981 cagtaaatgg tataatgatt atgctgtaag tgtgaaaagc aggatgtcta taaatccaga
     8041 tacttccaaa aaccagttct ctcttcagct taatagtgta actcccgaag acacagctgt
     8101 ttactattgt gcgcggggta tgatgactta ctactatggt atggacgttt ggggacaagg
     8161 tacaaccgtc accgtgtcca gtgggatcct gggctcaggc ggtggaggtt ccggtggggg
     8221 cgggtccgga ggggggggta gccaacctgt tctcacgcaa tcatcctccc tcagtgcaag
     8281 ccccggtgcc tccgcttctt tgacgtgtac actgaggtca ggaatcaatg tagggcctta
     8341 caggatctac tggtatcagc aaaaaccagg ttctcctccc caataccttc tcaattacaa
     8401 gagcgactca gataagcagc agggatctgg agttccatca aggtttagtg gatctaaaga
     8461 cgcaagcgcc aatgcgggtg tgctgctcat ctcaggtctc aggtccgagg acgaggccga
     8521 ttattactgt atgatttggc atagttcagc cgctgttttc ggtggaggca cgcaactgac
     8581 agtgctgacc acgacgccag cgccgcgacc accaacaccg gcgcccacca tcgcgtcgca
     8641 gcccctgtcc ctgcgcccag aggcgtgccg gccagcggcg gggggcgcag tgcacacgag
     8701 ggggctggac ttcgcctgtg atttttgggt gctg
//
