LOCUS       sequence-44423-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6644 MSCV-P C-Flag HA 45E7-Kozak
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..38)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(12..38)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(54..77)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(452..476)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(505..524)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(506..524)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(552..568)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(584..606)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gtagaattct aggcgtagtc gggcacgtcg taggggtatc ctccagcggc cgacttgtca
       61 tcgtcgtcct tgtagtcctc gagttcgaac cgcgggccct ctagatcaac cactttgtac
      121 aagaaagttg gttggttagt tgcacaccac ggacacacaa aggacaaggt gctcaaaaac
      181 agctgctgta gtgttctaag gtcctctgcc gagctctcta ctgtaagctc aattctgccg
      241 tcacacttac aacatacaca caaaattttg tgacgctgtg gttcggctcg tcgggctggt
      301 agttgtgcat gactaactcc atctgcttca tcgttttcct cctctgactc gcttaattgc
      361 tcgtaacaca acaggtcaac aggatctaat tcattctgag gttccaaatg caatacaatt
      421 tcttgcagtg tttcccgggg tccatgcatg gtgccaactt ttttgtacaa acttgttgat
      481 agcttaacta gccagcttgg gtctccctat agtgagtcgt attagatcta attccggcgc
      541 ctagagaagg agtgagggct ggataaaggg aggatcgagg cggggtcgaa cgaggaggtt
      601 caagggggag agacggggcg gatggaggaa gaggaggcgg aggcttaggg tgtacaaagg
      661 gcttgaccca gggagggggg tcaaaagcca aggcttccca ggtcacgat
//
