LOCUS       sequence-444235-        5194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of the type I CBASS system encoded by Xanthomonas 
            phaseoli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Richmond-Buccola D, Hobbs SJ, Garcia JM, Toyoda H, Gao J, Shao S, 
            Lee ASY, Kranzusch PJ
  TITLE     A large-scale type I CBASS antiphage screen identifies the phage 
            prohead protease as a key determinant of immune activation and 
            evasion.
  JOURNAL   Cell Host Microbe. 2024 Jul 10;32(7):1074-1088.e5. doi: 
            10.1016/j.chom.2024.05.021. Epub 2024 Jun 24.
  PUBMED    38917809
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2024.05.021"; journalName: "Cell Host Microbe"; 
            date: "2024-07-10- 10"; volume: "32"; issue: "7"; pages: 
            "1074-1088.e5"
COMMENT     Sequence Label: pBAD02_CBASS_Xanthomonas_phaseoli
FEATURES             Location/Qualifiers
     source          1..5194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     140..162
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(318..1196)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1223..1507
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1430..1449
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3033..3104
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3165..3192
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3211..3302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3303..3371,3372..4163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3521..3540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4334..4922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4823..4842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5076..5093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(5108..5194,1..54)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5174..5193)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
       61 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      121 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
      181 aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc
      241 tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt
      301 gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa
      361 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag
      421 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc
      481 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct
      541 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg
      601 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt
      661 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac
      721 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg
      781 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag
      841 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga
      901 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga
      961 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga
     1021 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc
     1081 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc
     1141 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt
     1201 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt
     1261 cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc
     1321 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat
     1381 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca
     1441 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
     1501 tctccatacc cgttttttgg gctaacagga ggaattaacc atgggcgacc agtaccttaa
     1561 tgggattctc gcgcgcgagg ccgttgatgt gtctttgctc tcacctgcat tctcagcgaa
     1621 ggcaacgctg acgccggtaa tcaatgcctg gggcggcaac ctagtgcagt cggttagtcc
     1681 aagcggatcg ttcgcgaagg gtacggcgaa cgccagcggc accgatgtgg atctgtttat
     1741 ctccttgcat ccctctacca cccacggcct cagggagatt tacatcacgc tcttcaatgc
     1801 tctgagagat gttggctaca cggtacgtaa gcaggacgtg tccctaggca tacgcgtggg
     1861 tctgatcgat gtggatctgg ttcccggaaa gcaacagacg ccactcacca ccgaccatag
     1921 cctctatcgc agccgggctg atacatggac caagaccaac gttcaaactc acatcgacgt
     1981 ggtccgcgca gccggacggc agtctgagac gagaatcatc aagctttggc gagatcagaa
     2041 caagttggaa ttcccatcgg tctacttgga attcgtggtg atcagggcct tgcagttgaa
     2101 cttctccggc agtttggccg agcgggtttg gacggtactg cggttcttgg ccgacaattt
     2161 tgcggctgtt cgagtcgttg atccggctaa tggaagcaat gtgatttcgg acgaccttac
     2221 gctcgcagag aagcttgcta tcgagcgcgc cgcgaaattt tcgcttacgc aacagacatg
     2281 gggcagcatc gtccgatgac acaggcatcg ctagctaaac ttctggatca cctgcacgta
     2341 caggtggagg cagagctgaa gactgcccgc tcagcgttgc atcaccccac cgacaagggc
     2401 gacgccagtg agcaggtttg gatcaactta ctgagcaact acttgcccag gcgctacgtg
     2461 gtgagcaaag cgcatgtggt cgatagcgag ggtaacttca gccagcagat cgatgtggtc
     2521 gtacacgatc gccagtattc cccattggta ttcaccttcc agggattgca ttatgtgcct
     2581 gcggaaagtg tctatgcgat ctttgaggcc aagcaagaga ccaacgcgca aacggtgaac
     2641 tatgcgcaga ccaaagcaga gagcgtgagg aggctgatca ggactagcat gccagtgccc
     2701 acggtagacg gccccaagcc ggccaaggaa ccgggccaca tcctggccgg tctgctgtca
     2761 ttgagctgcc cttggaagcc tccgctagga gaggctttgg cctgccatgt tcacgccagg
     2821 ggcggggact cgcagctcga tatgggctgt atcgcagatg cgggtatatt tttcatgaac
     2881 gatgagggtg cgctggaagt ccagcctgga gcacgcgcag caacgcgatt cctcctcgag
     2941 ctaatagcca ggttgcagga gctgggaaca gttccgatgc tggatatccg cagttacgcc
     3001 agacacatcc tgtaataaga attcccaggc atcaaataaa acgaaaggct cagtcgaaag
     3061 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctccagctg gtaccatatg
     3121 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac
     3181 ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata
     3241 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3301 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3361 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3421 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3481 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3541 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3601 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3661 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3721 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3781 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3841 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3901 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3961 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4021 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4081 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4141 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4201 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4261 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4321 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4381 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4441 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4501 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4561 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4621 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4681 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4741 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4801 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4861 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4921 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4981 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5041 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5101 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     5161 ggtgcactct cagtacaatc tgctctgatg ccgc
//
