LOCUS       sequence-44424-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6645 MSCV-P C-Flag HA 57E7-Kozak
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..49)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(23..49)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(65..88)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    120..144
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(421..445)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(474..493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(475..493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(521..537)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(553..575)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ctccctaccc ggtagaattc taggcgtagt cgggcacgtc gtaggggtat cctccagcgg
       61 ccgacttgtc atcgtcgtcc ttgtagtcct cgagttcgaa ccgcgggccc tctagatcaa
      121 ccactttgta caagaaagtt ggtacgcagc gggggcacac tatggtcaac gtattcagga
      181 acagctgctc cagatgcctt atgtccgccg ctccgcactc aaccaccagc cggactgtac
      241 tgtggcactt acagcacgtc gttaccaccc cgtaggcctg cacagctggt tctgtcagtt
      301 gatagttagt atcttcttct gagttgtcaa attgctcgtc gcaatgtagg tcaacaattt
      361 cgggtatttc ttccaatatt agtgtgatgt cctcaaggct ggggcgttct ccatgcatgg
      421 tgccaacttt tttgtacaaa cttgttgata gcttaactag ccagcttggg tctccctata
      481 gtgagtcgta ttagatctaa ttccggcgcc tagagaagga gtgagggctg gataaaggga
      541 ggatcgaggc ggggtcgaac gaggaggttc aagggggaga gacggggcgg atggaggaag
      601 aggaggcgga ggcttagggt gtacaaaggg cttgacccag ggaggggggt caaaagccaa
      661 ggcttcccag gtcacgatg
//
