LOCUS       sequence-444251-        4917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication
            origin), p15A (E. coli replication origin).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Feng J, Qian Y, Zhou Z, Ertmer S, Vivas EI, Lan F, Hamilton JJ, Rey 
            FE, Anantharaman K, Venturelli OS
  TITLE     Polysaccharide utilization loci in Bacteroides determine population 
            fitness and community-level interactions.
  JOURNAL   Cell Host Microbe. 2022 Feb 9;30(2):200-215.e12. doi: 
            10.1016/j.chom.2021.12.006. Epub 2022 Jan 6.
  PUBMED    34995484
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2021.12.006"; journalName: "Cell Host Microbe"; 
            date: "2022-02-9- 9"; volume: "30"; issue: "2"; pages: "200-215.e12"
COMMENT     Sequence Label: pFW2000
FEATURES             Location/Qualifiers
     source          1..4917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            102..211
                     /note="incP origin of transfer"
     rep_origin      276..821
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     722..741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     938..955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     980..996
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     980..996
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(4028..4096,4097..4888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4246..4265)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caattcgagg gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
       61 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
      121 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
      181 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
      241 gtctacacga accctttgtt aataagatga tcttcttgag atcgttttgg tctgcgcgta
      301 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga
      361 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc
      421 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc
      481 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt
      541 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga
      601 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
      661 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag
      721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
      781 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc
      841 tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta
      901 agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
      961 ggaatatatc cgcggccgct ctagaactag tggatccccc gggctgcagg aattcgatat
     1021 caagcttatc gataccgtcg atgtcgacta attgcctatc ttccagtgat aactatctat
     1081 gaaactgttt gatactttta ggtacctacc cggagtgttt cagccgggta atcttgttac
     1141 ttatccttga ttttcccttt aagggcgttt ataatccacc ctttcggatt gttcttttct
     1201 cgtgattttc cgtttaggag agccagttct ccgataaggt cggttatctt ttcttgtgcc
     1261 gttatgaatg tctctttgtt ccggtttatc tcttccgatg tgaagccgca ggaataacgg
     1321 aggtactcgt acacatggct gtctatctga tatcgtgctg taacctttgc ttgcaattct
     1381 ttcccttcca gttcttcatc tctgaactgt ggttgataga ccgggtagaa cctaaacccg
     1441 gtcaccttgc tacgtttgtt gtttgggttt tcacgcactt tcacgtagtt gaaagtgtat
     1501 gggcagctct catcaagcgc agcctttgct ggtttcaaaa ctctttcctc aaaatgatct
     1561 attctgtctt ttccgttctt gtccttgtat ttgtccgcag gtatgccaag acggtctttc
     1621 aagttttcaa gggatatatc caaagggtac acctgcccac tcattaacat gtaaaaccta
     1681 agggaatacc cagtaggtag cgcaagagcc ttgtttaact caaattctct gtaccccttg
     1741 gcgaacttgg taaacacatc ccaaaggtcg ttagacacac gaaaggttat aatgccagtg
     1801 cgttttttat attttgggtt agatataaat ccgcacttcc accattcctc gtcatcttcg
     1861 taagtaaaaa aacgtcctgc caaagaatcg agtgcggcta tgatttcatt gtaatcacgt
     1921 ccggaaaaaa taacgtcaga tacatgcatt tgtgcatcaa catcccaaag tccatgctca
     1981 aacttgcgtt tcgtgcctgc ataatctttc agttttactc cctttagttc agcttggcac
     2041 gcttccagta tgcgaagaac tattctttgt tcgtgtatgg acatgtcttg ctgttttgac
     2101 catgtatata cccatgaaac aacaacatct ttattcttgg taataggtag tttctttttc
     2161 ataaacgatt gatttgctgc aaatatacac aatccccaca ttaaaacaaa aaaagagggg
     2221 taaaactccc aaagtgtggg gactaattta cttttaaact cccaaagtgt ggggactaaa
     2281 ctcccaaagt gtggggaaac acatgttcta atccctttat ttatgggcgt ttgcgaagaa
     2341 gtgatagagc atattacaga cgatgcttat aacagacaag gggaatttat agagctaagt
     2401 ggttgattta ctgtcggaaa aagaaaaacg atagggaaaa agagcaaagc aaaaccaacc
     2461 aacgggcggt aggattggaa acgggttttt atggcgtgta cacgtttgtg tacaaaatgt
     2521 ttgtatttta ttgaaatata gctatctttg tgctgaattt aaaagcaaac agatatggaa
     2581 gcattatcag taagagaata ccgtaacaac ctcgcagcgt ctttcaccaa agctgacaat
     2641 ggcgagcagg tactaattcg caggaaaaac gagatttacg ctttggtaaa agttggtcgt
     2701 gaagatttga tgataacccc ggagctgcaa gcaaggattg acaaggcaag ggaagaaggt
     2761 accgaggacg cgtaaacatt tacagttgca tgtggcctat tgtttttagc cgttaaatat
     2821 tttataacta ttaaatagcg atacaaattg ttcgaaacta atattgttta tatcatatat
     2881 tctcgcatgt tttaaagctt tattaaattg attttttgta aacagttttt cgtactcttt
     2941 gttaacccat ttcattacaa aagtttcata tttttttctc tctttaaatg ccatttttgc
     3001 tggctttctt tttaatacaa ttaatgtgct atccacttta ggttttggat ggaaataata
     3061 cctaggaatt tttgctaata tagaaatatc tacctctgcc attaacagca atgctagtga
     3121 tctgtttgta tctaataaca ttttagcaaa accatattcc actattaaat aacttattgt
     3181 ggctgaactt tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt aaggtatgct
     3241 gccaaatatt ttatatggat tgtggctagg aaatgtaaat ttcagtatat catcatttac
     3301 tatttgatag ttaggataat ttaagagctt attacgagtt acctcacata atttagaatc
     3361 aatttctatc gccgttacaa aattacatct ctttaccaat ccagcagtaa aatgaccttt
     3421 ccctgcacct atttcaaaga tgttatcttt ttcatctaaa cttatgcaat tcattatttt
     3481 ttctatgtga tattttgaag taataaaatt ttgactatct tttatattta ctttgttcat
     3541 tataacctct ccttaattta ttgcatctct tttcgaatat ttatgttttt tgagaaaaga
     3601 acgtactcat ggttcatccc gatatgcgta tcggtctgta tatcagcaac tttctatgtg
     3661 tttcaactac aatagtcatc tattctcatc tttctgagtc caccccctgc aaagcccctc
     3721 tttacgacat aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct ctttaagaga
     3781 aactcttcgg gatgcaaaaa tatgaaaata actccaattc accaaattat atagcgactt
     3841 ttttacaaaa tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca aaaatgtctt
     3901 ttacgataaa atgatacctc aatatcaact gtttagcaaa acgatatttc tcttaaagag
     3961 agaaacacct ttttgttcac caatccccga cttttaatcc cgcggccatg attgaaaaag
     4021 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4081 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4141 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4201 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4261 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4321 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4381 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4441 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4501 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4561 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4621 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4681 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4741 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4801 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4861 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat aacgcgt
//
