LOCUS       sequence-444252-       10654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteroides genomic editing tool; IPTG inducible-Fncpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Feng J, Qian Y, Zhou Z, Ertmer S, Vivas EI, Lan F, Hamilton JJ, Rey 
            FE, Anantharaman K, Venturelli OS
  TITLE     Polysaccharide utilization loci in Bacteroides determine population 
            fitness and community-level interactions.
  JOURNAL   Cell Host Microbe. 2022 Feb 9;30(2):200-215.e12. doi: 
            10.1016/j.chom.2021.12.006. Epub 2022 Jan 6.
  PUBMED    34995484
REFERENCE   2  (bases 1 to 10654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2021.12.006"; journalName: "Cell Host Microbe"; 
            date: "2022-02-9- 9"; volume: "30"; issue: "2"; pages: "200-215.e12"
COMMENT     Sequence Label: pFW2100
FEATURES             Location/Qualifiers
     source          1..10654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            102..211
                     /note="incP origin of transfer"
     rep_origin      276..821
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     722..741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     938..955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     980..996
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     980..996
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(998..1083)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1236..1253
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     protein_bind    5239..5255
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5272..5288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5664..6746
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5683..5702)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(9765..9833,9834..10625)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9983..10002)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caattcgagg gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
       61 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
      121 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
      181 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
      241 gtctacacga accctttgtt aataagatga tcttcttgag atcgttttgg tctgcgcgta
      301 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga
      361 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc
      421 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc
      481 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt
      541 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga
      601 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
      661 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag
      721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
      781 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc
      841 tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta
      901 agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
      961 ggaatatatc cgcggccgct ctagaactag tggatccttt gtcctactca ggagagcgtt
     1021 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     1081 ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac
     1141 ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag
     1201 gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa
     1261 tcttctctca tccgccaaaa cagccaagct tttagttgtt acggttctga acgaactcaa
     1321 aatattcttc gtttttaata accagattaa gtttcttacc ctcctggtta tttttaatac
     1381 ggcccaggag cataagtcct ttcagaccaa tgtgataagc tccatttgca tctgcgtctt
     1441 gtggcatatt tttcggcgcc tggcggctgt caaagaagtt cccgttcaca tccgccaccg
     1501 gcgagatcag ataatcgagt tcggtacctg tcttggaatt gcgcatctga aggatagtat
     1561 ttaatacgct ggtgagtttg gcgaaaaact ttttatcgct ttcaccgcaa attgcggctt
     1621 taatacactc accgtgacca tattcgatcg agtagtcttt gagcagtttc tccagctctt
     1681 tcgtaggata cacttcgcgg gtatcccaat tatgattttt gtccgagttg cgaaagttaa
     1741 tcaggcggct gccgaaggag gcaatcgtcc acttgccttt cgcggctttg tcgccgaagt
     1801 ttttgtagtc gaagctgaac tcgaaatagc ccttatccag gttgtagcag attttatcaa
     1861 acttgctaaa gaactcctgg ctcttggata cactttcata ctttgggtac agttggttga
     1921 cgaagcccgt gaccgggcaa atcttgctgg tgaagcctgc cggcacgtaa tagataatac
     1981 ccgtctgttt acccattttc ttgaaggttt cgaacggggc ggtcagctga tacgcacgca
     2041 gaacgccacc tgtcttatca aactcattgt ccttgaagac cagatagttc agtttctcaa
     2101 tcaacatttt ctctaatttc tggtaaacct gtttttcgac cttgaagcga ccgcgtttga
     2161 aaccaaagtt cagatcttca aacactacaa tagcattata ttcaatgact aatttggcga
     2221 tttcgtgtac tacctgagaa agataacctt ctttcatttc cttgatgttg ttgatttttt
     2281 tccagtcttt gcgagcgcta tcacgatctt tctcgatagc agccagtttg tcgtgataat
     2341 tcgttttcat gcgatcatta ccgatgatat tgaaggtatc ctgcttgata atgttaccct
     2401 tgccatccac cagcgtgtaa tacgccagat ggcgttcacc acgatcgatc gacagaatgt
     2461 gcacatcgtt cgctttctct ttcagcagca agttaatttc gtcgttaaat ttgtttgcgc
     2521 cggacgattt aaagttaatc gtgatcgggc agtgaaagaa aaacttatct tccgtaaagc
     2581 gtttgtcttt aatcaggtcg tactcgaaca cgctttcctt tttcggattg tccttgtttt
     2641 tgttggcaat cgcttccttc gccgggtggg taattttctt tggaatgctc tgtttacgat
     2701 aaaacagttc cgcctcgcca ttgagtttgt acactacatc ctggagatta cgctcatcaa
     2761 acaatgcctt ccaatataag gtatggagat tggggcggcc ctttgagtac gcgctgaaat
     2821 ctttattata aatctggaag aggtacagtt tgccctgatt tacaacggag tcaatgtatg
     2881 actcgctgat gttttcgaat gtcagtttat aaccctgatt ttcgacctcg cgatagaact
     2941 catcgatcga gttataacgt tgggtgtcgg agaaacggaa gccaaagtct ttccattcgg
     3001 gatgtttgct gatcgattgt ttgtaaaagt cgataaactt acgacaatct tcaatattga
     3061 actcaaactt ttcatagcct ttttgcgggc tgccattttt ggtatgcgtc gaatgattac
     3121 ggatgcgcag gatgtcttca ctcggattat aaaatttaat gctcttggcg ctaaaaaaca
     3181 ctttcggcag cattttgttc gccccaggca gcagtttata aacgattttc ttgtaacctt
     3241 cacccttatt ttccttgatg gctttgtcgt caaagatttt gttgtttttc ttgttcatga
     3301 cgcctaaata atatttatcg tctttgataa acagaatggc ggtattatct ggctctttat
     3361 tcttatccca accgttcgcc agggtggagt tttcgaaatt cagtttgaat ttttcatcgc
     3421 tatagggttt ctgagtaata taattgcgaa ttttattata gagggggaca atgtttgcta
     3481 attcgaagta gcattcttcg aaaaccaggt agaagtgttc atctttatcg agaatgttcg
     3541 ccttgtcttc tgactggcta atgtgaaaga tcttcagttt atgcagcaga ttattagttt
     3601 ggtccagcag gtctttgatc gctttcacat catcttcggc gctagcctgc aggagatctt
     3661 tcttaccctg attctggtat ttaattgaga tctgcgccag attgtcctta ttctgtgcga
     3721 tttcatcgaa aatcatcgga atggcggcaa aattggccag gatttcttcg aaacggcatt
     3781 gtttgtcgat atcacgatgt ttgttaaatt cttccagggc cagtttaatg gtttccaggc
     3841 tcagatattt cgctttttcc gtttttttcg caatcagttc ctgttctttc ttacttgggt
     3901 tatctaagtt tttcggagca atttgctggg taatatattc cagcacggca gtaccaatca
     3961 cggagtagtc atcaaacacc tgttgggaca ggtcggttaa agacttatcg tttttaaagt
     4021 agattttcga aagatccagc ttctgggctt tgagatcatc aaataagagg ctgagcgtct
     4081 ctttgatcga cttctcttcg acggttttga aagccgcaat ctgttcataa aatgactgca
     4141 tagtcgtcac tacatcggag tcatcttcca gtttgtcaat cacaaatgac ttactttccg
     4201 tatcacttaa aatctgtttg aacagcacgg acatcttata ttttttgagt gttttatcgt
     4261 tgatttgctg gctatacaga ttaatgtatt cgttgatacc tttacgtttg gtgttttcac
     4321 cgttaacgaa tttcccgcca ataatcgtat taaacttagt aatcccggat tgatttaagt
     4381 agttattaaa gtttgcaatc tcgaaaactt cgtccaggct aaagacgcgc tggttcactt
     4441 ccgaggtttt atagtcaata tcaaaagtca actcctccgc caagtccttt ttgatctgtt
     4501 cataattgat cgcttccggc gccttatcct tcagagattc atatttcgct ttgttctcca
     4561 aaaatttggg taaattatcg tcaacgatgc ggtaaatgat agaggttggg atgtcgtttg
     4621 agctgtacac gtttttacga ttttcatgaa atcctttgaa gtaggttgtc caccctttga
     4681 acgatttgat aatttccagt gcttcatcga tatctgtgat gtcgctattt gccttaaaca
     4741 gttcgatgcc gttatctttg gattgtttaa gccacaggat cagatcggat tcctgccctt
     4801 tcttggcatc gatcaggttc tggttgaaca ggtttttgaa cttctcggaa tccttaatgt
     4861 actcactaat ctgtttcttg atagtatcct tggcactctt aaaatctttc tgcagattat
     4921 catcgtccga tttcttcagc ttgaagtaaa catcgctata gttctgaaga aggtcttcac
     4981 tgatacacac ggagctaaga atttcttcaa taaaaaactg atgatatttg tcgatgattt
     5041 gtttagcttt cttgtagtct tttgcacgct tttcgtcatc caggataagg ccacgcgctt
     5101 tgatgttttc cagcgttttg ccttgtggaa ttaattcaaa gcgcagcgtt ttcgacaggg
     5161 aatatttgtt tacgaattcc tgatagatac tcatgtttat attatttata tttgtttgac
     5221 gagaacatct ttatttaatt gttatccgct cacaattgcc gacaaaggta attgttatcc
     5281 gctcacaatt caaataaata gatagaaaaa taacagtttg tcgaattttc tttgtaagcg
     5341 gccgctgatc tggaagaagc aatgaaagct gctgttaagt ctccgaatca ggtattgttc
     5401 ctgacaggtg tattcccatc cggtaaacgc ggatactttg cagttgatct gactcaggaa
     5461 taaattataa attaaggtaa gaagattgta ggataagcta atgaaataga aaaaggatgc
     5521 cgtcacacaa cttgtcggca ttcttttttg ttttattagt tgaaaatata gtgaaaaagt
     5581 tgcctaaata tgtatgttaa caaattattt gtcgtaactt tgcactccaa atctgttttt
     5641 aacatatggc actagtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg
     5701 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
     5761 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg
     5821 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc
     5881 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca
     5941 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca
     6001 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg
     6061 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc
     6121 agacacccat caacagtatt attttctccc atgaggacgg tacgcgactg ggcgtggagc
     6181 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct
     6241 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga
     6301 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc
     6361 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg
     6421 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat
     6481 acgacgatac cgaggacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt
     6541 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     6601 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca
     6661 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6721 tttcccgact ggaaagcggg cagtgagaat tcgatatcaa gcttatcgat accgtcgatg
     6781 tcgactaatt gcctatcttc cagtgataac tatctatgaa actgtttgat acttttaggt
     6841 acctacccgg agtgtttcag ccgggtaatc ttgttactta tccttgattt tccctttaag
     6901 ggcgtttata atccaccctt tcggattgtt cttttctcgt gattttccgt ttaggagagc
     6961 cagttctccg ataaggtcgg ttatcttttc ttgtgccgtt atgaatgtct ctttgttccg
     7021 gtttatctct tccgatgtga agccgcagga ataacggagg tactcgtaca catggctgtc
     7081 tatctgatat cgtgctgtaa cctttgcttg caattctttc ccttccagtt cttcatctct
     7141 gaactgtggt tgatagaccg ggtagaacct aaacccggtc accttgctac gtttgttgtt
     7201 tgggttttca cgcactttca cgtagttgaa agtgtatggg cagctctcat caagcgcagc
     7261 ctttgctggt ttcaaaactc tttcctcaaa atgatctatt ctgtcttttc cgttcttgtc
     7321 cttgtatttg tccgcaggta tgccaagacg gtctttcaag ttttcaaggg atatatccaa
     7381 agggtacacc tgcccactca ttaacatgta aaacctaagg gaatacccag taggtagcgc
     7441 aagagccttg tttaactcaa attctctgta ccccttggcg aacttggtaa acacatccca
     7501 aaggtcgtta gacacacgaa aggttataat gccagtgcgt tttttatatt ttgggttaga
     7561 tataaatccg cacttccacc attcctcgtc atcttcgtaa gtaaaaaaac gtcctgccaa
     7621 agaatcgagt gcggctatga tttcattgta atcacgtccg gaaaaaataa cgtcagatac
     7681 atgcatttgt gcatcaacat cccaaagtcc atgctcaaac ttgcgtttcg tgcctgcata
     7741 atctttcagt tttactccct ttagttcagc ttggcacgct tccagtatgc gaagaactat
     7801 tctttgttcg tgtatggaca tgtcttgctg ttttgaccat gtatataccc atgaaacaac
     7861 aacatcttta ttcttggtaa taggtagttt ctttttcata aacgattgat ttgctgcaaa
     7921 tatacacaat ccccacatta aaacaaaaaa agaggggtaa aactcccaaa gtgtggggac
     7981 taatttactt ttaaactccc aaagtgtggg gactaaactc ccaaagtgtg gggaaacaca
     8041 tgttctaatc cctttattta tgggcgtttg cgaagaagtg atagagcata ttacagacga
     8101 tgcttataac agacaagggg aatttataga gctaagtggt tgatttactg tcggaaaaag
     8161 aaaaacgata gggaaaaaga gcaaagcaaa accaaccaac gggcggtagg attggaaacg
     8221 ggtttttatg gcgtgtacac gtttgtgtac aaaatgtttg tattttattg aaatatagct
     8281 atctttgtgc tgaatttaaa agcaaacaga tatggaagca ttatcagtaa gagaataccg
     8341 taacaacctc gcagcgtctt tcaccaaagc tgacaatggc gagcaggtac taattcgcag
     8401 gaaaaacgag atttacgctt tggtaaaagt tggtcgtgaa gatttgatga taaccccgga
     8461 gctgcaagca aggattgaca aggcaaggga agaaggtacc gaggacgcgt aaacatttac
     8521 agttgcatgt ggcctattgt ttttagccgt taaatatttt ataactatta aatagcgata
     8581 caaattgttc gaaactaata ttgtttatat catatattct cgcatgtttt aaagctttat
     8641 taaattgatt ttttgtaaac agtttttcgt actctttgtt aacccatttc attacaaaag
     8701 tttcatattt ttttctctct ttaaatgcca tttttgctgg ctttcttttt aatacaatta
     8761 atgtgctatc cactttaggt tttggatgga aataatacct aggaattttt gctaatatag
     8821 aaatatctac ctctgccatt aacagcaatg ctagtgatct gtttgtatct aataacattt
     8881 tagcaaaacc atattccact attaaataac ttattgtggc tgaactttca aaaacaattt
     8941 ttcgaattat atttgtgctt atgttgtaag gtatgctgcc aaatatttta tatggattgt
     9001 ggctaggaaa tgtaaatttc agtatatcat catttactat ttgatagtta ggataattta
     9061 agagcttatt acgagttacc tcacataatt tagaatcaat ttctatcgcc gttacaaaat
     9121 tacatctctt taccaatcca gcagtaaaat gacctttccc tgcacctatt tcaaagatgt
     9181 tatctttttc atctaaactt atgcaattca ttattttttc tatgtgatat tttgaagtaa
     9241 taaaattttg actatctttt atatttactt tgttcattat aacctctcct taatttattg
     9301 catctctttt cgaatattta tgttttttga gaaaagaacg tactcatggt tcatcccgat
     9361 atgcgtatcg gtctgtatat cagcaacttt ctatgtgttt caactacaat agtcatctat
     9421 tctcatcttt ctgagtccac cccctgcaaa gcccctcttt acgacataaa aattcggtcg
     9481 gaaaaggtat gcaaaagatg tttctctctt taagagaaac tcttcgggat gcaaaaatat
     9541 gaaaataact ccaattcacc aaattatata gcgacttttt tacaaaatgc taaaatttgt
     9601 tgatttccgt caagcaattg ttgagcaaaa atgtctttta cgataaaatg atacctcaat
     9661 atcaactgtt tagcaaaacg atatttctct taaagagaga aacacctttt tgttcaccaa
     9721 tccccgactt ttaatcccgc ggccatgatt gaaaaaggaa gagtatgagt attcaacatt
     9781 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9841 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9901 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9961 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    10021 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10081 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10141 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10201 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10261 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10321 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10381 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10441 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10501 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10561 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10621 ggtaactgtc agaccaagtt tactcataac gcgt
//
