LOCUS       sequence-444253-       11607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Feng J, Qian Y, Zhou Z, Ertmer S, Vivas EI, Lan F, Hamilton JJ, Rey 
            FE, Anantharaman K, Venturelli OS
  TITLE     Polysaccharide utilization loci in Bacteroides determine population 
            fitness and community-level interactions.
  JOURNAL   Cell Host Microbe. 2022 Feb 9;30(2):200-215.e12. doi: 
            10.1016/j.chom.2021.12.006. Epub 2022 Jan 6.
  PUBMED    34995484
REFERENCE   2  (bases 1 to 11607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2021.12.006"; journalName: "Cell Host Microbe"; 
            date: "2022-02-9- 9"; volume: "30"; issue: "2"; pages: "200-215.e12"
COMMENT     Sequence Label: pFW2500
FEATURES             Location/Qualifiers
     source          1..11607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            102..211
                     /note="incP origin of transfer"
     rep_origin      276..821
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     722..741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     938..955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     980..996
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     980..996
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(998..1083)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1236..1253
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     protein_bind    5239..5255
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5272..5288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5664..6746
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5683..5702)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    7640..7656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    7673..7689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(10718..10786,10787..11578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10936..10955)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caattcgagg gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
       61 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
      121 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
      181 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
      241 gtctacacga accctttgtt aataagatga tcttcttgag atcgttttgg tctgcgcgta
      301 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga
      361 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc
      421 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc
      481 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt
      541 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga
      601 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
      661 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag
      721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
      781 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc
      841 tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta
      901 agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
      961 ggaatatatc cgcggccgct ctagaactag tggatccttt gtcctactca ggagagcgtt
     1021 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     1081 ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac
     1141 ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag
     1201 gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa
     1261 tcttctctca tccgccaaaa cagccaagct tttagttgtt acggttctga acgaactcaa
     1321 aatattcttc gtttttaata accagattaa gtttcttacc ctcctggtta tttttaatac
     1381 ggcccaggag cataagtcct ttcagaccaa tgtgataagc tccatttgca tctgcgtctt
     1441 gtggcatatt tttcggcgcc tggcggctgt caaagaagtt cccgttcaca tccgccaccg
     1501 gcgagatcag ataatcgagt tcggtacctg tcttggaatt gcgcatctga aggatagtat
     1561 ttaatacgct ggtgagtttg gcgaaaaact ttttatcgct ttcaccgcaa attgcggctt
     1621 taatacactc accgtgacca tattcgatcg agtagtcttt gagcagtttc tccagctctt
     1681 tcgtaggata cacttcgcgg gtatcccaat tatgattttt gtccgagttg cgaaagttaa
     1741 tcaggcggct gccgaaggag gcaatcgtcc acttgccttt cgcggctttg tcgccgaagt
     1801 ttttgtagtc gaagctgaac tcgaaatagc ccttatccag gttgtagcag attttatcaa
     1861 acttgctaaa gaactcctgg ctcttggata cactttcata ctttgggtac agttggttga
     1921 cgaagcccgt gaccgggcaa atcttgctgg tgaagcctgc cggcacgtaa tagataatac
     1981 ccgtctgttt acccattttc ttgaaggttt cgaacggggc ggtcagctga tacgcacgca
     2041 gaacgccacc tgtcttatca aactcattgt ccttgaagac cagatagttc agtttctcaa
     2101 tcaacatttt ctctaatttc tggtaaacct gtttttcgac cttgaagcga ccgcgtttga
     2161 aaccaaagtt cagatcttca aacactacaa tagcattata ttcaatgact aatttggcga
     2221 tttcgtgtac tacctgagaa agataacctt ctttcatttc cttgatgttg ttgatttttt
     2281 tccagtcttt gcgagcgcta tcacgatctt tctcgatagc agccagtttg tcgtgataat
     2341 tcgttttcat gcgatcatta ccgatgatat tgaaggtatc ctgcttgata atgttaccct
     2401 tgccatccac cagcgtgtaa tacgccagat ggcgttcacc acgatcgatc gacagaatgt
     2461 gcacatcgtt cgctttctct ttcagcagca agttaatttc gtcgttaaat ttgtttgcgc
     2521 cggacgattt aaagttaatc gtgatcgggc agtgaaagaa aaacttatct tccgtaaagc
     2581 gtttgtcttt aatcaggtcg tactcgaaca cgctttcctt tttcggattg tccttgtttt
     2641 tgttggcaat cgcttccttc gccgggtggg taattttctt tggaatgctc tgtttacgat
     2701 aaaacagttc cgcctcgcca ttgagtttgt acactacatc ctggagatta cgctcatcaa
     2761 acaatgcctt ccaatataag gtatggagat tggggcggcc ctttgagtac gcgctgaaat
     2821 ctttattata aatctggaag aggtacagtt tgccctgatt tacaacggag tcaatgtatg
     2881 actcgctgat gttttcgaat gtcagtttat aaccctgatt ttcgacctcg cgatagaact
     2941 catcgatcga gttataacgt tgggtgtcgg agaaacggaa gccaaagtct ttccattcgg
     3001 gatgtttgct gatcgattgt ttgtaaaagt cgataaactt acgacaatct tcaatattga
     3061 actcaaactt ttcatagcct ttttgcgggc tgccattttt ggtatgcgtc gaatgattac
     3121 ggatgcgcag gatgtcttca ctcggattat aaaatttaat gctcttggcg ctaaaaaaca
     3181 ctttcggcag cattttgttc gccccaggca gcagtttata aacgattttc ttgtaacctt
     3241 cacccttatt ttccttgatg gctttgtcgt caaagatttt gttgtttttc ttgttcatga
     3301 cgcctaaata atatttatcg tctttgataa acagaatggc ggtattatct ggctctttat
     3361 tcttatccca accgttcgcc agggtggagt tttcgaaatt cagtttgaat ttttcatcgc
     3421 tatagggttt ctgagtaata taattgcgaa ttttattata gagggggaca atgtttgcta
     3481 attcgaagta gcattcttcg aaaaccaggt agaagtgttc atctttatcg agaatgttcg
     3541 ccttgtcttc tgactggcta atgtgaaaga tcttcagttt atgcagcaga ttattagttt
     3601 ggtccagcag gtctttgatc gctttcacat catcttcggc gctagcctgc aggagatctt
     3661 tcttaccctg attctggtat ttaattgaga tctgcgccag attgtcctta ttctgtgcga
     3721 tttcatcgaa aatcatcgga atggcggcaa aattggccag gatttcttcg aaacggcatt
     3781 gtttgtcgat atcacgatgt ttgttaaatt cttccagggc cagtttaatg gtttccaggc
     3841 tcagatattt cgctttttcc gtttttttcg caatcagttc ctgttctttc ttacttgggt
     3901 tatctaagtt tttcggagca atttgctggg taatatattc cagcacggca gtaccaatca
     3961 cggagtagtc atcaaacacc tgttgggaca ggtcggttaa agacttatcg tttttaaagt
     4021 agattttcga aagatccagc ttctgggctt tgagatcatc aaataagagg ctgagcgtct
     4081 ctttgatcga cttctcttcg acggttttga aagccgcaat ctgttcataa aatgactgca
     4141 tagtcgtcac tacatcggag tcatcttcca gtttgtcaat cacaaatgac ttactttccg
     4201 tatcacttaa aatctgtttg aacagcacgg acatcttata ttttttgagt gttttatcgt
     4261 tgatttgctg gctatacaga ttaatgtatt cgttgatacc tttacgtttg gtgttttcac
     4321 cgttaacgaa tttcccgcca ataatcgtat taaacttagt aatcccggat tgatttaagt
     4381 agttattaaa gtttgcaatc tcgaaaactt cgtccaggct aaagacgcgc tggttcactt
     4441 ccgaggtttt atagtcaata tcaaaagtca actcctccgc caagtccttt ttgatctgtt
     4501 cataattgat cgcttccggc gccttatcct tcagagattc atatttcgct ttgttctcca
     4561 aaaatttggg taaattatcg tcaacgatgc ggtaaatgat agaggttggg atgtcgtttg
     4621 agctgtacac gtttttacga ttttcatgaa atcctttgaa gtaggttgtc caccctttga
     4681 acgatttgat aatttccagt gcttcatcga tatctgtgat gtcgctattt gccttaaaca
     4741 gttcgatgcc gttatctttg gattgtttaa gccacaggat cagatcggat tcctgccctt
     4801 tcttggcatc gatcaggttc tggttgaaca ggtttttgaa cttctcggaa tccttaatgt
     4861 actcactaat ctgtttcttg atagtatcct tggcactctt aaaatctttc tgcagattat
     4921 catcgtccga tttcttcagc ttgaagtaaa catcgctata gttctgaaga aggtcttcac
     4981 tgatacacac ggagctaaga atttcttcaa taaaaaactg atgatatttg tcgatgattt
     5041 gtttagcttt cttgtagtct tttgcacgct tttcgtcatc caggataagg ccacgcgctt
     5101 tgatgttttc cagcgttttg ccttgtggaa ttaattcaaa gcgcagcgtt ttcgacaggg
     5161 aatatttgtt tacgaattcc tgatagatac tcatgtttat attatttata tttgtttgac
     5221 gagaacatct ttatttaatt gttatccgct cacaattgcc gacaaaggta attgttatcc
     5281 gctcacaatt caaataaata gatagaaaaa taacagtttg tcgaattttc tttgtaagcg
     5341 gccgctgatc tggaagaagc aatgaaagct gctgttaagt ctccgaatca ggtattgttc
     5401 ctgacaggtg tattcccatc cggtaaacgc ggatactttg cagttgatct gactcaggaa
     5461 taaattataa attaaggtaa gaagattgta ggataagcta atgaaataga aaaaggatgc
     5521 cgtcacacaa cttgtcggca ttcttttttg ttttattagt tgaaaatata gtgaaaaagt
     5581 tgcctaaata tgtatgttaa caaattattt gtcgtaactt tgcactccaa atctgttttt
     5641 aacatatggc actagtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg
     5701 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
     5761 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg
     5821 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc
     5881 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca
     5941 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca
     6001 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg
     6061 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc
     6121 agacacccat caacagtatt attttctccc atgaggacgg tacgcgactg ggcgtggagc
     6181 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct
     6241 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga
     6301 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc
     6361 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg
     6421 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat
     6481 acgacgatac cgaggacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt
     6541 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     6601 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca
     6661 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6721 tttcccgact ggaaagcggg cagtgagaat tcgatatcaa gcttatcgat accgtcgatg
     6781 tcgacttatt cctctgaatt atcgattaca ctgtattccc cggttaatac agaggaatct
     6841 gcaggatcga ttgtcagtgg ttccttttca tccattgata ctgcacgctg gatctcaatt
     6901 gatacgggca aatatttgaa caggcgacga atagccgttt tctttgccat ttcttcccag
     6961 tgagttaccc acggcccgtt attaccagct ttactcaggc tgcgcaccag ctcaatctgt
     7021 ttgcgcgtca taacttcaaa ctgagtacct ccgtctttca gtcttgcgac agcatagacg
     7081 tgggtaaccg gggcatcttc gttttctccc gggcggtgta ttaacttttc atcaaggcca
     7141 aattcgaagc taaactcgtc accttcacgg acaacacggg ctgacaggct ggcgatttga
     7201 ccagaacggc gagccagatc aatcatgccg cgatagccaa tgattagctg aacgttcttt
     7261 ttaccgctct tttcgttttt attaccaaaa ggcagtaaat atgcatgacc gagggcgcta
     7321 cctggctcaa gtccgagctg tgaacactgt acgatcgcac tgacaaaact catagtgtca
     7381 cagtttccta acgccggaac tttacgaatt tctgtggtgg cgatacggat catacgttca
     7441 gccgtcatat ggcgtggaag agctgctgcc agttgctctt tcattgatgg ctggttaata
     7501 aaactaatca cgtcgctatt tttaactgct gctggtgcac ggtttccctg agttttttgc
     7561 agatcggctt ttgcgattgg tggttgctta gtcatgttta tattatttat atttgtttga
     7621 cgagaatatc tttatttaat tgttatccgc tcacaattgc cgacaaaggt aattgttatc
     7681 cgctcacaat tcaaataaat agatagaaaa ataacagttt gtcgaatttt ctttgtaata
     7741 attgcctatc ttccagtgat aactatctat gaaactgttt gatactttta ggtacctacc
     7801 cggagtgttt cagccgggta atcttgttac ttatccttga ttttcccttt aagggcgttt
     7861 ataatccacc ctttcggatt gttcttttct cgtgattttc cgtttaggag agccagttct
     7921 ccgataaggt cggttatctt ttcttgtgcc gttatgaatg tctctttgtt ccggtttatc
     7981 tcttccgatg tgaagccgca ggaataacgg aggtactcgt acacatggct gtctatctga
     8041 tatcgtgctg taacctttgc ttgcaattct ttcccttcca gttcttcatc tctgaactgt
     8101 ggttgataga ccgggtagaa cctaaacccg gtcaccttgc tacgtttgtt gtttgggttt
     8161 tcacgcactt tcacgtagtt gaaagtgtat gggcagctct catcaagcgc agcctttgct
     8221 ggtttcaaaa ctctttcctc aaaatgatct attctgtctt ttccgttctt gtccttgtat
     8281 ttgtccgcag gtatgccaag acggtctttc aagttttcaa gggatatatc caaagggtac
     8341 acctgcccac tcattaacat gtaaaaccta agggaatacc cagtaggtag cgcaagagcc
     8401 ttgtttaact caaattctct gtaccccttg gcgaacttgg taaacacatc ccaaaggtcg
     8461 ttagacacac gaaaggttat aatgccagtg cgttttttat attttgggtt agatataaat
     8521 ccgcacttcc accattcctc gtcatcttcg taagtaaaaa aacgtcctgc caaagaatcg
     8581 agtgcggcta tgatttcatt gtaatcacgt ccggaaaaaa taacgtcaga tacatgcatt
     8641 tgtgcatcaa catcccaaag tccatgctca aacttgcgtt tcgtgcctgc ataatctttc
     8701 agttttactc cctttagttc agcttggcac gcttccagta tgcgaagaac tattctttgt
     8761 tcgtgtatgg acatgtcttg ctgttttgac catgtatata cccatgaaac aacaacatct
     8821 ttattcttgg taataggtag tttctttttc ataaacgatt gatttgctgc aaatatacac
     8881 aatccccaca ttaaaacaaa aaaagagggg taaaactccc aaagtgtggg gactaattta
     8941 cttttaaact cccaaagtgt ggggactaaa ctcccaaagt gtggggaaac acatgttcta
     9001 atccctttat ttatgggcgt ttgcgaagaa gtgatagagc atattacaga cgatgcttat
     9061 aacagacaag gggaatttat agagctaagt ggttgattta ctgtcggaaa aagaaaaacg
     9121 atagggaaaa agagcaaagc aaaaccaacc aacgggcggt aggattggaa acgggttttt
     9181 atggcgtgta cacgtttgtg tacaaaatgt ttgtatttta ttgaaatata gctatctttg
     9241 tgctgaattt aaaagcaaac agatatggaa gcattatcag taagagaata ccgtaacaac
     9301 ctcgcagcgt ctttcaccaa agctgacaat ggcgagcagg tactaattcg caggaaaaac
     9361 gagatttacg ctttggtaaa agttggtcgt gaagatttga tgataacccc ggagctgcaa
     9421 gcaaggattg acaaggcaag ggaagaaggt accgaggacg cgtaaacatt tacagttgca
     9481 tgtggcctat tgtttttagc cgttaaatat tttataacta ttaaatagcg atacaaattg
     9541 ttcgaaacta atattgttta tatcatatat tctcgcatgt tttaaagctt tattaaattg
     9601 attttttgta aacagttttt cgtactcttt gttaacccat ttcattacaa aagtttcata
     9661 tttttttctc tctttaaatg ccatttttgc tggctttctt tttaatacaa ttaatgtgct
     9721 atccacttta ggttttggat ggaaataata cctaggaatt tttgctaata tagaaatatc
     9781 tacctctgcc attaacagca atgctagtga tctgtttgta tctaataaca ttttagcaaa
     9841 accatattcc actattaaat aacttattgt ggctgaactt tcaaaaacaa tttttcgaat
     9901 tatatttgtg cttatgttgt aaggtatgct gccaaatatt ttatatggat tgtggctagg
     9961 aaatgtaaat ttcagtatat catcatttac tatttgatag ttaggataat ttaagagctt
    10021 attacgagtt acctcacata atttagaatc aatttctatc gccgttacaa aattacatct
    10081 ctttaccaat ccagcagtaa aatgaccttt ccctgcacct atttcaaaga tgttatcttt
    10141 ttcatctaaa cttatgcaat tcattatttt ttctatgtga tattttgaag taataaaatt
    10201 ttgactatct tttatattta ctttgttcat tataacctct ccttaattta ttgcatctct
    10261 tttcgaatat ttatgttttt tgagaaaaga acgtactcat ggttcatccc gatatgcgta
    10321 tcggtctgta tatcagcaac tttctatgtg tttcaactac aatagtcatc tattctcatc
    10381 tttctgagtc caccccctgc aaagcccctc tttacgacat aaaaattcgg tcggaaaagg
    10441 tatgcaaaag atgtttctct ctttaagaga aactcttcgg gatgcaaaaa tatgaaaata
    10501 actccaattc accaaattat atagcgactt ttttacaaaa tgctaaaatt tgttgatttc
    10561 cgtcaagcaa ttgttgagca aaaatgtctt ttacgataaa atgatacctc aatatcaact
    10621 gtttagcaaa acgatatttc tcttaaagag agaaacacct ttttgttcac caatccccga
    10681 cttttaatcc cgcggccatg attgaaaaag gaagagtatg agtattcaac atttccgtgt
    10741 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    10801 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    10861 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    10921 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
    10981 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    11041 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    11101 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    11161 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    11221 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    11281 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    11341 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    11401 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    11461 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    11521 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    11581 gtcagaccaa gtttactcat aacgcgt
//
