LOCUS       sequence-444256-        4408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG-inducible phage phi-X174 lysis gene E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Cheng YY, Zhou Z, Papadopoulos JM, Zuke JD, Falbel TG, Anantharaman 
            K, Burton BM, Venturelli OS
  TITLE     Efficient plasmid transfer via natural competence in a microbial 
            co-culture.
  JOURNAL   Mol Syst Biol. 2023 Mar 9;19(3):e11406. doi: 10.15252/msb.202211406.
            Epub 2023 Jan 30.
  PUBMED    36714980
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2023-03-9"; volume: "19(3):e11406. doi"; pages: " 
            10.15252/msb.202211406. Epub 2023 Jan 30"
COMMENT     Sequence Label: pYC01
FEATURES             Location/Qualifiers
     source          1..4408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    434..450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    467..483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      895..981
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1010..1028)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1024..1071
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1242..2033)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2034..2125)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(3186..4268)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4230..4249
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4269..4346)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     join(4392..4408,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(4392..4408,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggctcgag gtattctgac catctaagta catgcgttaa ttatcaggcc
      121 cgctattggc gggccttttt tatttgtgca gcaccccggt caaccaacgg gaaaattcac
      181 gcatcgcggg cgtctccggg cgagattgca aacgcgttat ccagtagctt cccaaatcaa
      241 tctgcgttaa aaacggctga acgatacgtt cactgctgag taaatgcgtg aacattctga
      301 ctggcgcaat tgccacaccc attcccgcct gtgcagcttc caacatggtg actgacgaat
      361 caaacaccat cacattgtgc gtcggtgacg gaggcgcctc tccggccgca aatttatcaa
      421 aaagagtgtt gacttgtgag cggataacaa tgatacttag attcaattgt gagcggataa
      481 caatttcaca catctgacgg gcccggacac ccttttgctt gaattcgata cacgtttcca
      541 agaggtaccc atggtacgct ggactttgtg ggataccctc gctttcctgc tcctgttgag
      601 tttattgctg ccgtcattgc ttattatgtt catcccgtca acattcaaac ggcctgtctc
      661 atcatggaag gcgctgaatt tacggaaaac attattaatg gcgtcgagcg tccggttaaa
      721 gccgctgaat tgttcgcgtt taccttgcgt gtacgcgcag gaaacactga cgttcttact
      781 gacgcagaag aaaacgtgcg tcaaaaatta cgtgcggaag gagtgaaagc ttggatccct
      841 gcaggcctca gggcccgatc gatgcggccg cttaattaat taatctagag gcatcaaata
      901 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      961 gctctcctga gtaggacaaa tccgccgccc tagacctagg ctgctgccac cgctgagcaa
     1021 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaacct
     1081 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     1141 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcatcgtgg
     1201 ccggatcttg cggcccctcg gcttgaacga attgttagac attatttgcc gactaccttg
     1261 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     1321 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     1381 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     1441 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     1501 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     1561 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     1621 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     1681 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     1741 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     1801 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     1861 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     1921 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     1981 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaatagct agctcactcg gtcgctacgc tccgggcgtg
     2161 agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag
     2221 gggactaaca tgtgaggcaa aacagcaggg ccgcgccggt ggcgtttttc cataggctcc
     2281 gccctcctgc cagagttcac ataaacagac gcttttccgg tgcatctgtg ggagccgtga
     2341 ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg
     2401 tttccggcgg gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac
     2461 ctgttccgcc tttctccctt acgggaagtg tggcgctttc tcatagctca cacactggta
     2521 tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac tccccgttca
     2581 gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgg
     2641 taaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta
     2701 aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg
     2761 gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc
     2821 ttcgatcaaa ccacctcccc aggtggtttt ttcgaaggtt aagtcagttg gggaactgct
     2881 taaccttcga tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg
     2941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaacc gctctagatt
     3001 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
     3061 taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
     3121 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
     3181 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     3241 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     3301 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     3361 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     3421 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg
     3481 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     3541 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     3601 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     3661 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     3721 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     3781 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     3841 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     3901 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     3961 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     4021 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     4081 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     4141 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     4201 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     4261 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     4321 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
     4381 cattaggaaa ttaatacgac tcactata
//
