LOCUS       sequence-44426-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6649 MSCV-P C-FlagHA 38 E7-Kozak
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..41)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(15..41)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(57..80)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    112..136
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(437..461)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(490..509)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(491..509)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(537..553)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(569..591)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ccggtagaat tctaggcgta gtcgggcacg tcgtaggggt atcctccagc ggccgacttg
       61 tcatcgtcgt ccttgtagtc ctcgagttcg aaccgcgggc cctctagatc aaccactttg
      121 tacaagaaag ttggtcgtcc gccattgcga atgtcttctc gacaggtggg acacagaagc
      181 cttacgtcgc ccagcaaaca atcttgcaga ttccgaatcc cagcgtcggt ggcccacacg
      241 tatagtttta gccttacttc acaaccccca caaagaacta tgattttgta tggggtgtat
      301 gctggctcct cctctaccac tgatgcttca atatcctctg gaagatcagg caactcctcg
      361 tggcaatgca ggtcaatggg ctggacaagc tcttcaagaa ctatatcacg aagagtagct
      421 tgtttcccaa tcatggtgcc aacttttttg tacaaacttg ttgatagctt aactagccag
      481 cttgggtctc cctatagtga gtcgtattag atctaattcc ggcgcctaga gaaggagtga
      541 gggctggata aagggaggat cgaggcgggg tcgaacgagg aggttcaagg gggagagacg
      601 gggcggatgg aggaagagga ggcggaggct tagggtgtac aaagggcttg acccagggag
      661 gggggtcaaa agc
//
