LOCUS       sequence-44427-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6651 MSCV-P C-Flag HA 76E7-Kozak
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..59)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    91..115
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(419..443)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(472..491)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(473..491)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(519..535)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(551..573)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tcgggcacgt cgtaggggta tcctccagcg gccgacttgt catcgtcgtc cttgtagtcc
       61 tcgagttcga accgcgggcc ctctagatca accactttgt acaagaaagt tggtcgtccg
      121 ccattgcgaa tctcttcacg acactccgga cacaggaatt gcaactcctc tagcagtaaa
      181 gattgaaatt ctcgaatggc ccgatcagta gccagcacaa acacacgaag tctagaaccg
      241 catccgccgc cacagcaagc aatcactttg taagggatgc cttggataag ctcctcctct
      301 gtgtcttgtt cagctggcac ttcagctggt aagttctcgt agcattgcag gtcaatgggc
      361 tggccaaact cttcttgtag cacaatgtca ggtaatgtag cctctttccc aatcatggtg
      421 ccaacttttt tgtacaaact tgttgatagc ttaactagcc agcttgggtc tccctatagt
      481 gagtcgtatt agatctaatt ccggcgccta gagaaggagt gagggctgga taaagggagg
      541 atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga cggggcggat ggaggaagag
      601 gaggcggang cttagggtgt acaaagggct tgacccaggg aggggggtca aaagccaagg
      661 cttccc
//
