LOCUS       sequence-44428-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6654 MSCV-P C-Flag HA 98E7-Kozak
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..44)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(18..44)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(60..83)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    115..139
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(425..449)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(478..497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(479..497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(525..541)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(557..579)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tacccggtag aattctaggc gtagtcgggc acgtcgtagg ggtatcctcc agcggccgac
       61 ttgtcatcgt cgtccttgta gtcctcgagt tcgaaccgcg ggccctctag atcaaccact
      121 ttgtacaaga aagttggttg tcctccatgc tgatcgctgt tacggcaatc cgggcacaat
      181 aagaccactt cttcaagtag cagctcctgc agggatcgta taccactgtg tgtggctgca
      241 acaaatattc gaagccgggt tccacagcct ccgccgcagc gcaaaattat tttgtaagat
      301 gtcctttcag gctccacctc cgtatcctgc tctggcaact cctcttcgca gtgcaaatca
      361 acaggctgtg gctcagtcaa ctctaagaca atgtcttgaa gagtgacctc ttttccaatc
      421 atggtgccaa cttttttgta caaacttgtt gatagcttaa ctagccagct tgggtctccc
      481 tatagtgagt cgtattagat ctaattccgg cgcctagaga aggagtgagg gctggataaa
      541 gggaggatcg aggcggggtc gaacgaggag gttcaagggg gagagacggg gcggatggag
      601 gaagaggagg cggaggctta gggtgtacaa agggcttgac ccagggaggg gggtcaaaag
      661 ccaaggcttc ccaggtcacg atgt
//
