LOCUS       sequence-444286-        3030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the 
            BBa_J23119 promoter, pSEVAb86 backbone, p15A ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   van den Brink M, Althuis TY, Danelon C, Claassens NJ
  TITLE     MOSAIC: A Highly Efficient, One-Step Recombineering Approach to 
            Plasmid Editing and Diversification.
  JOURNAL   ACS Synth Biol. 2025 Jun 5. doi: 10.1021/acssynbio.4c00657.
  PUBMED    40471122
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jun 5. doi: 10.1021/acssynbio.4c00657."
COMMENT     Sequence Label: pSEVAb867
FEATURES             Location/Qualifiers
     source          1..3030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     403..422
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     619..636
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(680..766)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    806..827
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        828..862
                     /label=BBa_J23118
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.56"
     RBS             871..882
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    1609..1629
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1655..1749
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1856..2656
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     oriT            2832..2940
                     /note="incP origin of transfer"
     rep_origin      join(2987..3030,1..502)
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
ORIGIN
        1 aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac tctttgaacc
       61 gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac
      121 cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt
      181 gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      241 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
      301 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa
      361 ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct
      421 ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc
      481 aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc cctgttaagt
      541 atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc cgctcgccgc
      601 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat ccggcgcgcc
      661 cagctgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
      721 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctttaattaa
      781 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagttg acggctagct
      841 cagtcctagg tattgtgcta gctactagag aaagaggaga aatactagat gcgtaaaggc
      901 gaagaactgt tcacgggcgt agttccgatt ctggtcgagc tggacggcga tgtgaacggt
      961 cataagttta gcgttcgcgg tgaaggtgag ggcgacgcga ccaacggcaa actgaccctg
     1021 aagttcatct gcaccaccgg taaactgccg gtgccttggc cgaccttggt gacgacgttg
     1081 acgtatggcg tgcagtgttt tgcgcgttat ccggaccaca tgaaacaaca cgatttcttc
     1141 aaatctgcga tgccggaggg ttacgtccag gagcgtacca tttccttcaa ggatgatggc
     1201 tactacaaaa ctcgcgcaga ggttaagttt gaaggtgaca cgctggtcaa tcgtatcgaa
     1261 ttgaagggta tcgactttaa agaggatggt aacattctgg gccataaact ggagtataac
     1321 ttcaacagcc ataatgttta cattacggca gacaagcaaa agaacggcat caaggccaat
     1381 ttcaagattc gccacaatgt tgaggacggt agcgtccaac tggccgacca ttaccagcag
     1441 aacaccccaa ttggtgacgg tccggttttg ctgccggata atcactatct gagcacccaa
     1501 agcgtgctga gcaaagatcc gaacgaaaaa cgtgatcaca tggtcctgct ggaatttgtg
     1561 accgctgcgg gcatcaccca cggtatggat gaactgtaca aataataata ctagtagcgg
     1621 ccgctgcagt ccggcaaaaa agggcaaggt cttggactcc tgttgataga tccagtaatg
     1681 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     1741 ggtgagaatc caggggtccc caataattac gatttaaatt tgtgtctcaa aatctctgat
     1801 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct ggaatatgtc
     1861 atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca
     1921 gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag
     1981 cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctgcgctggg
     2041 tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc
     2101 tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc
     2161 aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca
     2221 gatcatctct gatccattgc ccctgccacc tcactcgcct gcaagcccgg tcgcccgtgt
     2281 ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct
     2341 gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct
     2401 tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt
     2461 tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca
     2521 tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg
     2581 ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc
     2641 tcgccagtcg attggctgag ctcatagtcc aattggaccg cggtccgcgc gttgtccttt
     2701 tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt
     2761 ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa
     2821 cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc
     2881 actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg
     2941 taggccggcc ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg
     3001 gtctgcgcgt aatctcttgc tctgaaaacg
//
