LOCUS       sequence-444288-        3218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the 
            BBa_J23119 promoter, pSEVAb88 backbone, pUC ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   van den Brink M, Althuis TY, Danelon C, Claassens NJ
  TITLE     MOSAIC: A Highly Efficient, One-Step Recombineering Approach to 
            Plasmid Editing and Diversification.
  JOURNAL   ACS Synth Biol. 2025 Jun 5. doi: 10.1021/acssynbio.4c00657.
  PUBMED    40471122
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jun 5. doi: 10.1021/acssynbio.4c00657."
COMMENT     Sequence Label: pSEVAb887
FEATURES             Location/Qualifiers
     source          1..3218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     448..467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     701..718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    835..856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(890..976)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    1016..1035
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     RBS             1072..1083
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    1810..1830
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1856..1950
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             2057..2857
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     oriT            3033..3141
                     /note="incP origin of transfer"
     rep_origin      join(3177..3218,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caggcgcgcc cagctgtcta gggcggcgga tttgtcctac
      901 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      961 cctttcgttt tatttgatgc ctttaattaa tagctttcgc taaggatgat ttctggaatt
     1021 cgcggccgct tctagatagc tcagtcctag gtattgtgct agctactaga gaaagaggag
     1081 aaatactaga tgcgtaaagg cgaagaactg ttcacgggcg tagttccgat tctggtcgag
     1141 ctggacggcg atgtgaacgg tcataagttt agcgttcgcg gtgaaggtga gggcgacgcg
     1201 accaacggca aactgaccct gaagttcatc tgcaccaccg gtaaactgcc ggtgccttgg
     1261 ccgaccttgg tgacgacgtt gacgtatggc gtgcagtgtt ttgcgcgtta tccggaccac
     1321 atgaaacaac acgatttctt caaatctgcg atgccggagg gttacgtcca ggagcgtacc
     1381 atttccttca aggatgatgg ctactacaaa actcgcgcag aggttaagtt tgaaggtgac
     1441 acgctggtca atcgtatcga attgaagggt atcgacttta aagaggatgg taacattctg
     1501 ggccataaac tggagtataa cttcaacagc cataatgttt acattacggc agacaagcaa
     1561 aagaacggca tcaaggccaa tttcaagatt cgccacaatg ttgaggacgg tagcgtccaa
     1621 ctggccgacc attaccagca gaacacccca attggtgacg gtccggtttt gctgccggat
     1681 aatcactatc tgagcaccca aagcgtgctg agcaaagatc cgaacgaaaa acgtgatcac
     1741 atggtcctgc tggaatttgt gaccgctgcg ggcatcaccc acggtatgga tgaactgtac
     1801 aaataataat actagtagcg gccgctgcag tccggcaaaa aagggcaagg tcttggactc
     1861 ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt
     1921 tgccgccggg cgttttttat tggtgagaat ccaggggtcc ccaataatta cgatttaaat
     1981 ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     2041 taaaactgtc tggaatatgt catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg
     2101 aaaagccgag ctcatcggtc agcttctcaa ccttggggtt acccccggcg gtgtgctgct
     2161 ggtccacagc tccttccgta gcgtccggcc cctcgaagat gggccacttg gactgatcga
     2221 ggccctgcgt gctgcgctgg gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct
     2281 ggacgacgag ccgttcgatc ctgccacgtc gcccgttaca ccggaccttg gagttgtctc
     2341 tgacacattc tggcgcctgc caaatgtaaa gcgcagcgcc catccatttg cctttgcggc
     2401 agcggggcca caggcagagc agatcatctc tgatccattg cccctgccac ctcactcgcc
     2461 tgcaagcccg gtcgcccgtg tccatgaact cgatgggcag gtacttctcc tcggcgtggg
     2521 acacgatgcc aacacgacgc tgcatcttgc cgagttgatg gcaaaggttc cctatggggt
     2581 gccgagacac tgcaccattc ttcaggatgg caagttggta cgcgtcgatt atctcgagaa
     2641 tgaccactgc tgtgagcgct ttgccttggc ggacaggtgg ctcaaggaga agagccttca
     2701 gaaggaaggt ccagtcggtc atgcctttgc tcggttgatc cgctcccgcg acattgtggc
     2761 gacagccctg ggtcaactgg gccgagatcc gttgatcttc ctgcatccgc cagaggcggg
     2821 atgcgaagaa tgcgatgccg ctcgccagtc gattggctga gctcatagtc caattggacc
     2881 gcggtccgcg cgttgtcctt ttccgctgca taaccctgct tcggggtcat tatagcgatt
     2941 ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta
     3001 gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc
     3061 gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat
     3121 cctgctctgc gaggctggcc gtaggccggc cccgtagaaa agatcaaagg atcttcttga
     3181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaac
//
