LOCUS       sequence-44429-e         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6653 MSCV-P C-Flag HA 92E7-Kozak
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..50)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(24..50)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(66..89)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    121..145
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(419..443)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(472..491)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(473..491)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(519..535)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(551..573)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ctcccctacc cggtagaatt ctaggcgtag tcgggcacgt cgtaggggta tcctccagcg
       61 gccgacttgt catcgtcgtc cttgtagtcc tcgagttcga accgcgggcc ctctagatca
      121 accactttgt acaagaaagt tggtctgcca tttttgcagt caggacacag caacgctatt
      181 tcttcaagaa gtaggtcttg cagagttcta attccaaatt gggtacacga tgcaaaaatt
      241 cgaagcttaa ttccgcagcc accacacgag gaaactatct tgtagtctat tctttgaggt
      301 gcaggctcct cctcctgatt ttctgacaag tcttcgtcac aatgcaggtc aatgggctgg
      361 acaaggtctt gcagatccag cacaatatct ggtatagtag cctgtttccc aatcatggtg
      421 ccaacttttt tgtacaaact tgttgatagc ttaactagcc agcttgggtc tccctatagt
      481 gagtcgtatt agatctaatt ccggcgccta gagaaggagt gagggctgga taaagggagg
      541 atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga cggggcggat ggaggaagag
      601 gaggcggagg cttagggtgt acaaagggct tgacccaggg aggggggtca aaagccaagg
      661 cttcccaggt cacgatgt
//
