LOCUS       sequence-444290-        3820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the 
            BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   van den Brink M, Althuis TY, Danelon C, Claassens NJ
  TITLE     MOSAIC: A Highly Efficient, One-Step Recombineering Approach to 
            Plasmid Editing and Diversification.
  JOURNAL   ACS Synth Biol. 2025 Jun 5. doi: 10.1021/acssynbio.4c00657.
  PUBMED    40471122
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jun 5. doi: 10.1021/acssynbio.4c00657."
COMMENT     Sequence Label: pSEVAb837
FEATURES             Location/Qualifiers
     source          1..3820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(295..381)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    421..442
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        443..477
                     /label=BBa_J23118
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.56"
     RBS             486..497
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    1224..1244
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1270..1364
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1471..2271
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     oriT            2447..2555
                     /note="incP origin of transfer"
     CDS             complement(2566..3228)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      join(3229..3820,1..180)
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 aaaaaaattg atggggcaag gccgcaggcc gcgcagttgg agccggtggg tatgtggtcg
       61 aaggctgggt agccggtggg caatccctgt ggtcaagctc gtgggcaggc gcagcctgtc
      121 catcagcttg tccagcaggg ttgtccacgg gccgagcgaa gcgagccagc cggtggccgc
      181 tcgcggccat cgtccacata tccacgggct ggcaagggag cgcagcgacc gcgcagggcg
      241 aagcccggag agcaagcccg tagggggggc gcgcccagct gtctagggcg gcggatttgt
      301 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      361 ctgagccttt cgttttattt gatgccttta attaatagct ttcgctaagg atgatttctg
      421 gaattcgcgg ccgcttctag agttgacggc tagctcagtc ctaggtattg tgctagctac
      481 tagagaaaga ggagaaatac tagatgcgta aaggcgaaga actgttcacg ggcgtagttc
      541 cgattctggt cgagctggac ggcgatgtga acggtcataa gtttagcgtt cgcggtgaag
      601 gtgagggcga cgcgaccaac ggcaaactga ccctgaagtt catctgcacc accggtaaac
      661 tgccggtgcc ttggccgacc ttggtgacga cgttgacgta tggcgtgcag tgttttgcgc
      721 gttatccgga ccacatgaaa caacacgatt tcttcaaatc tgcgatgccg gagggttacg
      781 tccaggagcg taccatttcc ttcaaggatg atggctacta caaaactcgc gcagaggtta
      841 agtttgaagg tgacacgctg gtcaatcgta tcgaattgaa gggtatcgac tttaaagagg
      901 atggtaacat tctgggccat aaactggagt ataacttcaa cagccataat gtttacatta
      961 cggcagacaa gcaaaagaac ggcatcaagg ccaatttcaa gattcgccac aatgttgagg
     1021 acggtagcgt ccaactggcc gaccattacc agcagaacac cccaattggt gacggtccgg
     1081 ttttgctgcc ggataatcac tatctgagca cccaaagcgt gctgagcaaa gatccgaacg
     1141 aaaaacgtga tcacatggtc ctgctggaat ttgtgaccgc tgcgggcatc acccacggta
     1201 tggatgaact gtacaaataa taatactagt agcggccgct gcagtccggc aaaaaagggc
     1261 aaggtcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
     1321 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggg gtccccaata
     1381 attacgattt aaatttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     1441 atcatcatga acaataaaac tgtctggaat atgtcatcag cggtggagtg caatgtcgtg
     1501 caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc
     1561 ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc ggcccctcga agatgggcca
     1621 cttggactga tcgaggccct gcgtgctgcg ctgggtccgg gagggacgct cgtcatgccc
     1681 tcgtggtcag gtctggacga cgagccgttc gatcctgcca cgtcgcccgt tacaccggac
     1741 cttggagttg tctctgacac attctggcgc ctgccaaatg taaagcgcag cgcccatcca
     1801 tttgcctttg cggcagcggg gccacaggca gagcagatca tctctgatcc attgcccctg
     1861 ccacctcact cgcctgcaag cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt
     1921 ctcctcggcg tgggacacga tgccaacacg acgctgcatc ttgccgagtt gatggcaaag
     1981 gttccctatg gggtgccgag acactgcacc attcttcagg atggcaagtt ggtacgcgtc
     2041 gattatctcg agaatgacca ctgctgtgag cgctttgcct tggcggacag gtggctcaag
     2101 gagaagagcc ttcagaagga aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc
     2161 cgcgacattg tggcgacagc cctgggtcaa ctgggccgag atccgttgat cttcctgcat
     2221 ccgccagagg cgggatgcga agaatgcgat gccgctcgcc agtcgattgg ctgagctcat
     2281 agtccaattg gaccgcggtc cgcgcgttgt ccttttccgc tgcataaccc tgcttcgggg
     2341 tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc
     2401 aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg
     2461 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg
     2521 tgctcaacgg gaatcctgct ctgcgaggct ggccgtaggc cggccctacc ggcgcggcag
     2581 cgttacccgt gtcggcggct ccaacggctc gccatcgtcc agaaaacacg gctcatcggg
     2641 catcggcagg cgctgctgcc cgcgccgttc ccattcctcc gtttcggtca aggctggcag
     2701 gtctggttcc atgcccggaa tgccgggctg gctgggcggc tcctcgccgg ggccggtcgg
     2761 tagttgctgc tcgcccggat acagggtcgg gatgcggcgc aggtcgccat gccccaacag
     2821 cgattcgtcc tggtcgtcgt gatcaaccac cacggcggca ctgaacaccg acaggcgcaa
     2881 ctggtcgcgg ggctggcccc acgccacgcg gtcattgacc acgtaggccg acacggtgcc
     2941 ggggccgttg agcttcacga cggagatcca gcgctcggcc accaagtcct tgactgcgta
     3001 ttggaccgtc cgcaaagaac gtccgatgag cttggaaagt gtcttctggc tgaccaccac
     3061 ggcgttctgg tggcccatct gcgccacgag gtgatgcagc agcattgccg ccgtgggttt
     3121 cctcgcaata agcccggccc acgcctcatg cgctttgcgt tccgtttgca cccagtgacc
     3181 gggcttgttc ttggcttgaa tgccgatttc tctggactgc gtggccatgc ttatctccat
     3241 gcggtagggg tgccgcacgg ttgcggcacc atgcgcaatc agctgcaact tttcggcagc
     3301 gcgacaacaa ttatgcgttg cgtaaaagtg gcagtcaatt acagattttc tttaacctac
     3361 gcaatgagct attgcggggg gtgccgcaat gagctgttgc gtacccccct tttttaagtt
     3421 gttgattttt aagtctttcg catttcgccc tatatctagt tctttggtgc ccaaagaagg
     3481 gcacccctgc ggggttcccc cacgccttcg gcgcggctcc ccctccggca aaaagtggcc
     3541 cctccggggc ttgttgatcg actgcgcggc cttcggcctt gcccaaggtg gcgctgcccc
     3601 cttggaaccc ccgcactcgc cgccgtgagg ctcggggggc aggcgggcgg gcttcgccct
     3661 tcgactgccc ccactcgcat aggcttgggt cgttccaggc gcgtcaaggc caagccgctg
     3721 cgcggtcgct gcgcgagcct tgacccgcct tccacttggt gtccaaccgg caagcgaagc
     3781 gcgcaggccg caggccggag gcttttcccc agagaaaatt
//
