LOCUS       sequence-44430-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6656 MSCV-P C-Flag HA 25E7-Kozak
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..42)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(16..42)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(58..81)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    113..137
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             425..433
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(444..468)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(497..516)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(498..516)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(544..560)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(576..598)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 cccggtagaa ttctaggcgt agtcgggcac gtcgtagggg tatcctccag cggccgactt
       61 gtcatcgtcg tccttgtagt cctcgagttc gaaccgcggg ccctctagat caaccacttt
      121 gtacaagaaa gttggggatc cgccatgttt gcagttcccg cgacactccg gacacaacag
      181 ctgcagttct tcaattagaa ggttttgtag tgttctaata ccaaaatcag tggctttcac
      241 aaagatgcga agcttgacct cgcagcaacc acaaggcgca acaactttgt atggagttct
      301 gtcaatagca ggctcctcct ctgtttcctg atgctcagcc ggcaactcct cttcacaaaa
      361 caggtcaact ggttgtacct caggctgcaa ttcacttaac tctaatgtaa aatcttgcaa
      421 tgtgacctcc ttaccaatca tggtgccaac ttttttgtac aaacttgttg atagcttaac
      481 tagccagctt gggtctccct atagtgagtc gtattagatc taattccggc gcctagagaa
      541 ggagtgaggg ctggataaag ggaggatcga ggcggggtcg aacgaggagg ttcaaggggg
      601 agagacgggg cggatggagg aagaggaggc ggaggcttan ggtgtacaaa gggcttgacc
      661 canggagggg ggtcaaaagc caaggcttcc caggtcacga tgtaaggg
//
