LOCUS       sequence-444305-        5963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of iCre with an HA tag in neurons driven by human 
            synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Hochbaum DR, Hulshof L, Urke A, Wang W, Dubinsky AC, Farnsworth HC, 
            Hakim R, Lin S, Kleinberg G, Robertson K, Park C, Solberg A, Yang Y,
            Baynard C, Nadaf NM, Beron CC, Girasole AE, Chantranupong L, 
            Cortopassi MD, Prouty S, Geistlinger L, Banks AS, Scanlan TS, Datta 
            SR, Greenberg ME, Boulting GL, Macosko EZ, Sabatini BL
  TITLE     Thyroid hormone remodels cortex to coordinate body-wide metabolism 
            and exploration.
  JOURNAL   Cell. 2024 Oct 3;187(20):5679-5697.e23. doi: 
            10.1016/j.cell.2024.07.041. Epub 2024 Aug 22.
  PUBMED    39178853
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.07.041"; journalName: "Cell"; date: "2024-10-3-
            3"; volume: "187"; issue: "20"; pages: "5679-5697.e23"
COMMENT     Sequence Label: DRH001_scAAV-hSyn-iCre-HA
FEATURES             Location/Qualifiers
     source          1..5963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        195..642
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      656..665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             671..691
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             695..715
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             719..739
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1781..1807
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1886..1906)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     rep_origin      2767..3280
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2854..2873)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3064..3085
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3762..3781)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3881..3903
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3941..3959)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4027..4131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4132..4200,4201..4992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4350..4369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5163..5751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5652..5671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgttggccg attcattaat gcagcagctg cgcgctcgct cgctcactga ggccgcccgg
       61 gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc
      121 agagagggag tggggttcgg taccgcggcc gcacgcgttg tacagtgtct agactgcaga
      181 gggccctgcg tatgagtgca agtgggtttt aggaccagga tgaggcgggg tgggggtgcc
      241 tacctgacga ccgaccccga cccactggac aagcacccaa cccccattcc ccaaattgcg
      301 catcccctat cagagagggg gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca
      361 gcttcagcac cgcggacagt gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc
      421 agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc aaactcccct tcccggccac
      481 cttggtcgcg tccgcgccgc cgccggccca gccggaccgc accacgcgag gcgcgagata
      541 ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg actcagcgct gcctcagtct
      601 gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc agtcgagaag gatccgccac
      661 catgggtgga ccaaaaaaga agagaaaggt agacccgaag aaaaagcgta aggtcgatcc
      721 caagaagaag aggaaggtgt ccaacctgct gactgtgcac caaaacctgc ctgccctccc
      781 tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca
      841 ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg
      901 gtgcaagctg aacaacagga aatggttccc tgctgaacct gaggatgtga gggactacct
      961 cctgtacctg caagccagag gcctggctgt gaagaccatc caacagcacc tgggccagct
     1021 caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg ctgtgtccct
     1081 ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca agcaggccct
     1141 ggcctttgaa cgcactgact ttgaccaagt cagatccctg atggagaact ctgacagatg
     1201 ccaggacatc aggaacctgg ccttcctggg cattgcctac aacaccctgc tgcgcattgc
     1261 cgaaattgcc agaatcagag tgaaggacat ctcccgcacc gatggtggga gaatgctgat
     1321 ccacattggc aggaccaaga ccctggtgtc cacagctggt gtggagaagg ccctgtccct
     1381 gggggttacc aagctggtgg agagatggat ctctgtgtct ggtgtggctg atgaccccaa
     1441 caactacctg ttctgccggg tcagaaagaa tggtgtggct gccccttctg ccacctccca
     1501 actgtccacc cgggccctgg aagggatctt tgaggccacc caccgcctga tctatggtgc
     1561 caaggatgac tctgggcaga gatacctggc ctggtctggc cactctgcca gagtgggtgc
     1621 tgccagggac atggccaggg ctggtgtgtc catccctgaa atcatgcagg ctggtggctg
     1681 gaccaatgtg aacattgtga tgaactacat cagaaacctg gactctgaga ctggggccat
     1741 ggtgaggctg ctcgaggatg gggacggtgg aggcggaagt tacccatacg atgttccaga
     1801 ttacgcttaa tgaatcgaat tccgctcgag ataatcaacc tctggattac aaaatttgtg
     1861 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     1921 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     1981 aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2041 caggggctcg gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg
     2101 ctattgcttt atttgtaacc atctagcttt atttgtgaaa tttgtgatgc tattgcttta
     2161 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     2221 tttcaggttc agggggagat gtgggaggtt ttttaaagcg gccgcaggaa cccctagtga
     2281 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     2341 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgccagctgg
     2401 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2461 gaatggcgat tccgttgcaa tggctggcgg taatattgtt ctggatatta ccagcaaggc
     2521 cgatagtttg agttcttcta ctcaggcaag tgatgttatt actaatcaaa gaagtattgc
     2581 gacaacggtt aatttgcgtg atggacagac tcttttactc ggtggcctca ctgattataa
     2641 aaacacttct caggattctg gcgtaccgtt cctgtctaaa atccctttaa tcggcctcct
     2701 gtttagctcc cgctctgatt ctaacgagga aagcacgtta tacgtgctcg tcaaagcaac
     2761 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     2821 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     2881 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     2941 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3001 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3061 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3121 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3181 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaaatattt gcttatacaa
     3241 tcttcctgtt tttggggctt ttctgattat caaccggggt acatatgatt gacatgctag
     3301 ttttacgatt accgttcatc gattctcttg tttgctccag actctcaggc aatgacctga
     3361 tagcctttgt agagacctct caaaaatagc taccctctcc ggcatgaatt tatcagctag
     3421 aacggttgaa tatcatattg atggtgattt gactgtctcc ggcctttctc acccgtttga
     3481 atctttacct acacattact caggcattgc atttaaaata tatgagggtt ctaaaaattt
     3541 ttatccttgc gttgaaataa aggcttctcc cgcaaaagta ttacagggtc ataatgtttt
     3601 tggtacaacc gatttagctt tatgctctga ggctttattg cttaattttg ctaattcttt
     3661 gccttgcctg tatgatttat tggatgttgg aatcgcctga tgcggtattt tctccttacg
     3721 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     3781 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3841 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     3901 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     3961 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4021 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4081 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4141 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4201 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4261 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4321 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4381 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4441 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4501 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4561 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4621 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4681 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4741 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4801 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4861 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4921 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4981 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5041 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5101 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5161 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5221 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5281 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5341 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5401 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5461 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5521 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5581 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5641 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5701 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5761 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5821 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5881 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5941 atacgcaaac cgcctctccc cgc
//
