LOCUS       sequence-444306-        5300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of inactive delta-iCre (control) with an HA tag in 
            neurons driven by human synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Hochbaum DR, Hulshof L, Urke A, Wang W, Dubinsky AC, Farnsworth HC, 
            Hakim R, Lin S, Kleinberg G, Robertson K, Park C, Solberg A, Yang Y,
            Baynard C, Nadaf NM, Beron CC, Girasole AE, Chantranupong L, 
            Cortopassi MD, Prouty S, Geistlinger L, Banks AS, Scanlan TS, Datta 
            SR, Greenberg ME, Boulting GL, Macosko EZ, Sabatini BL
  TITLE     Thyroid hormone remodels cortex to coordinate body-wide metabolism 
            and exploration.
  JOURNAL   Cell. 2024 Oct 3;187(20):5679-5697.e23. doi: 
            10.1016/j.cell.2024.07.041. Epub 2024 Aug 22.
  PUBMED    39178853
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.07.041"; journalName: "Cell"; date: "2024-10-3-
            3"; volume: "187"; issue: "20"; pages: "5679-5697.e23"
COMMENT     Sequence Label: DRH002_scAAV-hSyn-delta_iCre-HA
FEATURES             Location/Qualifiers
     source          1..5300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        526..973
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      987..996
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1002..1022
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1026..1046
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1050..1070
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1449..1475
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1554..1574)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     rep_origin      2435..2948
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2522..2541)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2732..2753
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3430..3449)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3549..3571
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3609..3627)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3695..3799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3800..3868,3869..4660)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4018..4037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4831..5300,1..119)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
       61 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      121 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      181 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
      241 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
      301 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcagct
      361 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
      421 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggggttcg gtaccgcggc
      481 cgcacgcgtt gtacagtgtc tagactgcag agggccctgc gtatgagtgc aagtgggttt
      541 taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga
      601 caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa
      661 caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc
      721 cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc
      781 cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc
      841 agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg
      901 cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg
      961 cctgagagcg cagtcgagaa ggatccgcca ccatgggtgg accaaaaaag aagagaaagg
     1021 tagacccgaa gaaaaagcgt aaggtcgatc ccaagaagaa gaggaaggtg tccaacctgc
     1081 tgactgtgca ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga
     1141 agaacctgat ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc
     1201 tcctgtctgt gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc
     1261 ctgctgaacc tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg
     1321 tgaagaccat ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc
     1381 ctcgcccttc tgactccaat gctgtgtccc tggtgatgag gagaatcaga aagggtggag
     1441 gcggaagtta cccatacgat gttccagatt acgcttaatg aatcgaattc cgctcgagat
     1501 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     1561 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     1621 atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc
     1681 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     1741 gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat
     1801 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     1861 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     1921 ttaaagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     1981 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     2041 tcagtgagcg agcgagcgcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     2101 ttcccaacag ttgcgcagcc tgaatggcga atggcgattc cgttgcaatg gctggcggta
     2161 atattgttct ggatattacc agcaaggccg atagtttgag ttcttctact caggcaagtg
     2221 atgttattac taatcaaaga agtattgcga caacggttaa tttgcgtgat ggacagactc
     2281 ttttactcgg tggcctcact gattataaaa acacttctca ggattctggc gtaccgttcc
     2341 tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg ctctgattct aacgaggaaa
     2401 gcacgttata cgtgctcgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     2461 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2521 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2581 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2641 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2701 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2761 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2821 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2881 cttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt ctgattatca
     2941 accggggtac atatgattga catgctagtt ttacgattac cgttcatcga ttctcttgtt
     3001 tgctccagac tctcaggcaa tgacctgata gcctttgtag agacctctca aaaatagcta
     3061 ccctctccgg catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga
     3121 ctgtctccgg cctttctcac ccgtttgaat ctttacctac acattactca ggcattgcat
     3181 ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg
     3241 caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg
     3301 ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg gatgttggaa
     3361 tcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     3421 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     3481 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3541 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3601 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     3661 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3721 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3781 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3841 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3901 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3961 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4021 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4081 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4141 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4201 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4261 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4321 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4381 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4441 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4501 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4561 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4621 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4681 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4741 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5221 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5281 cagggtcgga acaggagagc
//
