LOCUS       sequence-444323-        5606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of S_DM transposase (CMV 
            promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pCMV-S_DM
FEATURES             Location/Qualifiers
     source          1..5606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1794..1812)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1795..1812)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1832..1849)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1838..2062
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2108..2536
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2195..2214)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2405..2426
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2545..2565)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2550..2879
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2730..2865
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2792..2811
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    3045..3166
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3082..3101)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3136..3155
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3215..3231)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3215..3231)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3228..3250)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3239..3255
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3263..3269,3270..3287,3288..3293))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3308..3329
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3446..3463)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3617..4205)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3697..3716)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4376..5167,5168..5236))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4999..5018
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5237..5341)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5416..5435)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg ccgccaccat
      721 gcctggtaaa cgcctggcgt ttgaagtgac ccagctgatc tattacaacc accagctggg
      781 caaaagcatc cctgagctgg tggagatctt tagcgtgagc cgcaaaaccg tgtataacat
      841 tctgaaccgc agcgaaaagg aaggtcgcct ggaacctaaa tcaggtggcg gctgcaagac
      901 caaaatcaac aaacgcgtgg accgcctgat aatgcgcaaa gcgattgcga accctcgtat
      961 tagcgttcgt agcctggcgc aggatattcg cgaagagtgc catctgaccg tgagccatga
     1021 aaccgtgcgc caggtgatat tacgccatcg ctattcatca cgcgtggcgc gtaaaaaacc
     1081 gctgctgagc gaaattaaca ttgagaagcg ccatagcttt gcggtgagca tgatggatca
     1141 tgcggaagag tattgggacg acgtgatctt ctgcgacgaa accaagatga tgctgtttta
     1201 caacgatggc ccgagccgtg tttggcgcaa acctttaagc gcgctggaaa cccagaacat
     1261 cattcctacc atcaagtttg gcaagctgag cgtgatgatc tggggctgca tcagctcaca
     1321 tggcgttggc aaactggcgt ttattgaaag caccatgaac gccgtgcagt acctggatat
     1381 tctgaagacc aacctgaaag cgagcgcgga aaaattcggc ctgttcagca acaacaaacc
     1441 gaactttaag ttctaccagg acaacgatcc taagcataag gaatacaacg tgcgcaactg
     1501 gctgctgtat aactgcggca aagtgattga taccccgcct cagagccctg atctgaaccc
     1561 gattgaaaac ctgtgggcct acctgaaaaa aaaagtggcg aagcgcggtc ctaaaacccg
     1621 ccagcagtta atggcggcga tcattgaaga atgggagaaa atccctctgg agtacgacct
     1681 gaaaaaactg atccacagca tgaaaaagcg cctgcagtta gtggcgaaag ccaacggtgg
     1741 ccataccaaa tattgatgag cggccgctcg agcatgcatc tagagggccc tattctatag
     1801 tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     1861 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1921 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     1981 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     2041 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     2101 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     2161 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2221 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2281 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     2341 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2401 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     2461 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     2521 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     2581 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     2641 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2701 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2761 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     2821 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     2881 agctccccga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     2941 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     3001 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     3061 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3121 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     3181 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     3241 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3301 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3361 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3421 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3481 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3541 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3601 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3661 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3721 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3781 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3841 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3901 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3961 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4021 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4081 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4141 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4201 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4261 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4321 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4381 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4441 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4501 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4561 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4621 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4681 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4741 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4801 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4861 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4921 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4981 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5041 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5101 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5161 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5221 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5281 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5341 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     5401 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     5461 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     5521 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     5581 cgatgtacgg gccagatata cgcgtt
//
