LOCUS       sequence-444352-        2158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0035
FEATURES             Location/Qualifiers
     source          1..2158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     157..175
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(179..222)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      356..413
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(437..454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(608..1196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(688..707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1379..1473)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1494..2153)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2068..2087
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
       61 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
      121 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
      181 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc ggtctcagga
      241 gggaggcgtg tcgttttagt gaggtccccg catagctgaa catctatata aggttttgct
      301 attcattgaa agcagtagtg actgatttgt atataatgta ctcgagaccc tgcagtccgg
      361 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
      421 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      481 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      541 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      601 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      661 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      721 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      781 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      841 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      901 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      961 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1021 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1081 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1141 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1201 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1261 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1321 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat
     1381 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1441 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1501 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1561 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     1621 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     1681 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     1741 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     1801 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     1861 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     1921 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     1981 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     2041 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     2101 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttag
//
